INS-1 cells inhibit the production of extracellular matrix from pancreatic stellate cells
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In type 2 diabetes mellitus, pancreatic stellate cells (PSCs) are present within and surrounding pancreatic islets and may cause progressive fibrosis and deterioration of pancreatic beta cell function. However, it is unknown whether pancreatic beta cells influence the biological behavior of PSCs. In the present study, we examined the impact of pancreatic beta cells on the proliferation, migration and extracellular matrix (ECM) production of PSCs. PSCs were treated with conditioned media from INS-1 cells (supernatant, SN). Although the proliferation of PSCs incubated with INS-1-SN was increased compared to control, INS-1-SN treatment induced matrix metalloproteinase-2 activity and reduced the production of ECM and TGF-β1. In addition, PSCs treated with INS-1-SN reduced the secretion of cytokines that are known to mediate pancreatic beta cell death, such as FADD, Fas, IFN-γ, IL-1, TNF-α, and TRAIL. Our findings suggest that pancreatic beta cells may ameliorate islet fibrosis and the progression of islet dysfunction.
KeywordsPancreatic stellate cells INS-1 cells Extracellular matrix Type 2 diabetes mellitus
The authors are grateful to Drs. Rennian Wang and Peter M. Jones for their critical comments on the manuscript. The authors thank Hansjoerg Habisch, Jing Lu and Gisela Sailer for technical assistance. The work was supported by the National Nature Science Foundation of China (Nos.: 30971399, 81170716 and 81270010).
Conflict of interest
The authors declare that they have no competing interests.
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