Early neurogenesis during caudal spinal cord regeneration in adult Gekko japonicus
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Gekko japonicus undergoes dramatic changes in the caudal spinal cord after tail amputation. The amputation induces cell proliferation in the caudal ependymal tube. We performed hematoxylin and eosin staining at different time points in the regeneration process to investigate the morphological characterization of the regenerated appendages. The central canal extended to the blastema post-amputation and the cartilage and muscle tissue appeared 3 weeks after injury. We performed the bromodeoxyuridine (BrdU) incorporation assay to detect proliferating cells during the regeneration process. BrdU positive cells were detected in the peri-central canal. Furthermore, nestin and neuron-specific enolase (NSE) immunocytochemistry were applied to detect neural stem/progenitor cells and neurons. Two weeks after injury, nestin-positive cells undergoing proliferation were located outside of the ependymal tube, and NSE positive cells appeared after 3 weeks of amputation. These data suggest that neurogenesis is an early event during caudal spinal cord regeneration in gecko.
KeywordsNeurogenesis Blastema Gekko japonicus Spinal cord Regeneration
This research supported by the grants from the NSFC (31071874, 31171007, 31171405), the Basic Research Program of Jiangsu Education Department (09KJA180005, 11KJA180004), Natural Science Foundation of Jiangsu Province (BK2010274) and the PAPD of Jiangsu Higher Education Institutions.
- Alibardi L (2010) Morphological and cellular aspects of tail and limb regeneration in lizards. A model system with implications for tissue regeneration in mammals. Adv Anat Embryol Cell Biol 207:iii, v–x, 1–109Google Scholar
- Benraiss A, Caubit X, Arsanto JP, Coulon J, Nicolas S, Le Parco Y, Thouveny Y (1996) Clonal cell cultures from adult spinal cord of the amphibian urodele Pleurodeles waltl to study the identity and potentialities of cells during tail regeneration. Dev Dyn Off Publ Am Assoc Anat 205(2):135–149Google Scholar
- Benraiss A, Arsanto JP, Coulon J, Thouveny Y (1997) Neural crest-like cells originate from the spinal cord during tail regeneration in adult amphibian urodeles. Dev Dyn Off Publ Am Assoc Anat 209(1):15–28Google Scholar
- Cox P (1969) Some aspects of tail regeneration in the lizard, Anolis carolinensis. I. A description based on histology and autoradiography. J Exp Zool 171(2):127–149Google Scholar
- Cristino L, Pica A, Della Corte F, Bentivoglio M (2000) Plastic changes and nitric oxide synthase induction in neurons which innervate the regenerated tail of the lizard Gekko gecko. II. The response of dorsal root ganglion cells to tail amputation and regeneration. Brain Res 871(1):83–93PubMedCrossRefGoogle Scholar
- Du S, Cheng L, Liu D (2002) Advances of systematic research of the genus gekko (sauria: gekkonidae). Sichuan J Zool 21:200–204Google Scholar
- Duffy MT, Liebich DR, Garner LK, Hawrych A, Simpson SB Jr, Davis BM (1992) Axonal sprouting and frank regeneration in the lizard tail spinal cord: correlation between changes in synaptic circuitry and axonal growth. J Comp neurol 316(3):363–374Google Scholar
- Fischer AH, Jacobson KA, Rose J, Zeller R (2008) Hematoxylin and eosin staining of tissue and cell sections. CSH Protoc 2008:pdb prot4986Google Scholar
- Kierdorf U, Kierdorf H (2012) Antler regrowth as a form of epimorphic regeneration in vertebrates—a comparative view. Front Biosci 4:1606–1624Google Scholar