Journal of Molecular Histology

, Volume 44, Issue 1, pp 37–45 | Cite as

Increased expression of actin filament-stabilizing protein tropomyosin after rat traumatic brain injury

Original Paper

Abstract

Tropomyosin (TM), is a coiled-coil dimmer which modulates actin filament properties, has been implicated in the control of actin filament dynamics during cell migration, morphogenesis, and cytokinesis. However, the expressions and possible functions of tropomyosin in central nervous system (CNS) lesion remain unknown. In this study, we found the expression of tropomyosin gradually increased in rat brains subjected to traumatic brain injury (TBI). Double immunofluorescence staining showed tropomyosin was expressed in neurons and reactive astrocytes following TBI but not in quiescent astrocytes in normal brains. Furthermore, we detected that proliferating cell nuclear antigen (PCNA) had the co-localization with GFAP, and tropomyosin. In conclusion, this was the first description of tropomyosin expression in rat traumatic brain. Our date suggested that tropomyosin might be involved in the astrocytes proliferation following TBI.

Keywords

Tropomyosin Traumatic brain injury Astrogliosis Rat 

Notes

Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81070992) and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

References

  1. Arifin MZ, Faried A, Shahib MN, Wiriadisastra K, Bisri T (2011) Inhibition of activated NR2B gene- and caspase-3 protein-expression by glutathione following traumatic brain injury in a rat model. Asian J Neurosurg 6:72–77PubMedCrossRefGoogle Scholar
  2. Balasubramanian MK, Helfman DM, Hemmingsen SM (1992) A new tropomyosin essential for cytokinesis in the fission yeast S. pombe. Nature 360:84–87PubMedCrossRefGoogle Scholar
  3. Becker EB, Bonni A (2004) Cell cycle regulation of neuronal apoptosis in development and disease. Prog Neurobiol 72:1–25PubMedCrossRefGoogle Scholar
  4. Blanchoin L, Pollard TD, Hitchcock-DeGregori SE (2001) Inhibition of the Arp2/3 complex-nucleated actin polymerization and branch formation by tropomyosin. Curr Biol 11:1300–1304PubMedCrossRefGoogle Scholar
  5. Bohmer RM, Scharf E, Assoian RK (1996) Cytoskeletal integrity is required throughout the mitogen stimulation phase of the cell cycle and mediates the anchorage-dependent expression of cyclin D1. Mol Biol Cell 7:101–111PubMedGoogle Scholar
  6. Chen J, Mao H, Zou H, Jin W, Ni L, et al. (2012) Up-regulation of ski-interacting protein in rat brain cortex after traumatic brain injury. J Mol HistolGoogle Scholar
  7. Chirumamilla S, Sun D, Bullock MR, Colello RJ (2002) Traumatic brain injury induced cell proliferation in the adult mammalian central nervous system. J Neurotrauma 19:693–703PubMedCrossRefGoogle Scholar
  8. Copani A, Uberti D, Sortino MA, Bruno V, Nicoletti F et al (2001) Activation of cell-cycle-associated proteins in neuronal death: a mandatory or dispensable path? Trends Neurosci 24:25–31PubMedCrossRefGoogle Scholar
  9. Di Giovanni S, Movsesyan V, Ahmed F, Cernak I, Schinelli S et al (2005) Cell cycle inhibition provides neuroprotection and reduces glial proliferation and scar formation after traumatic brain injury. Proc Natl Acad Sci USA 102:8333–8338PubMedCrossRefGoogle Scholar
  10. Fawcett JW (1997) Astrocytic and neuronal factors affecting axon regeneration in the damaged central nervous system. Cell Tissue Res 290:371–377PubMedCrossRefGoogle Scholar
  11. Ferrier R, Had L, Rabie A, Faivre-Sarrailh C (1994) Coordinated expression of five tropomyosin isoforms and beta-actin in astrocytes treated with dibutyryl cAMP and cytochalasin D. Cell Motil Cytoskeleton 28:303–316PubMedCrossRefGoogle Scholar
  12. Fujita T, Yoshimine T, Maruno M, Hayakawa T (1998) Cellular dynamics of macrophages and microglial cells in reaction to stab wounds in rat cerebral cortex. Acta Neurochir (Wien) 140:275–279CrossRefGoogle Scholar
  13. Galloway PG, Likavec MJ, Perry G (1990) Tropomyosin isoform expression in normal and neoplastic astrocytes. Lab Invest 62:163–170PubMedGoogle Scholar
  14. Had L, Faivre-Sarrailh C, Legrand C, Rabie A (1993) The expression of tropomyosin genes in pure cultures of rat neurons, astrocytes and oligodendrocytes is highly cell-type specific and strongly regulated during development. Brain Res Mol Brain Res 18:77–86PubMedCrossRefGoogle Scholar
  15. Hannan AJ, Schevzov G, Gunning P, Jeffrey PL, Weinberger RP (1995) Intracellular localization of tropomyosin mRNA and protein is associated with development of neuronal polarity. Mol Cell Neurosci 6:397–412PubMedCrossRefGoogle Scholar
  16. Hill-Felberg SJ, McIntosh TK, Oliver DL, Raghupathi R, Barbarese E (1999) Concurrent loss and proliferation of astrocytes following lateral fluid percussion brain injury in the adult rat. J Neurosci Res 57:271–279PubMedCrossRefGoogle Scholar
  17. Hughes JA, Cooke-Yarborough CM, Chadwick NC, Schevzov G, Arbuckle SM et al (2003) High-molecular-weight tropomyosins localize to the contractile rings of dividing CNS cells but are absent from malignant pediatric and adult CNS tumors. Glia 42:25–35PubMedCrossRefGoogle Scholar
  18. Jiang S, Wu X, Yan Y, Xu J, Shao B et al (2012) The expression changes of Numblike in rat brain cortex after traumatic brain injury. J Mol Histol 43:195–201PubMedCrossRefGoogle Scholar
  19. Kato H, Takahashi A, Itoyama Y (2003) Cell cycle protein expression in proliferating microglia and astrocytes following transient global cerebral ischemia in the rat. Brain Res Bull 60:215–221PubMedCrossRefGoogle Scholar
  20. Koguchi K, Nakatsuji Y, Nakayama K, Sakoda S (2002) Modulation of astrocyte proliferation by cyclin-dependent kinase inhibitor p27(Kip1). Glia 37:93–104PubMedCrossRefGoogle Scholar
  21. Kurahashi H, Imai Y, Yamamoto M (2002) Tropomyosin is required for the cell fusion process during conjugation in fission yeast. Genes Cells 7:375–384PubMedCrossRefGoogle Scholar
  22. Li W, Gao FB (2003) Actin filament-stabilizing protein tropomyosin regulates the size of dendritic fields. J Neurosci 23:6171–6175PubMedGoogle Scholar
  23. Little AR, O’Callagha JP (2001) Astrogliosis in the adult and developing CNS: is there a role for proinflammatory cytokines? Neurotoxicology 22:607–618PubMedCrossRefGoogle Scholar
  24. Liu DX, Greene LA (2001) Neuronal apoptosis at the G1/S cell cycle checkpoint. Cell Tissue Res 305:217–228PubMedCrossRefGoogle Scholar
  25. Liu Y, Wang Y, Cheng C, Chen Y, Shi S, Qin J, Xiao F, Zhou D, Lu M, Lu Q, Shen A (2010) A relationship between p27(kip1) and Skp2 after adult brain injury: implications for glial proliferation. J Neurotrauma 27:361–371PubMedCrossRefGoogle Scholar
  26. Logan A, Frautschy SA, Gonzalez AM, Baird A (1992) A time course for the focal elevation of synthesis of basic fibroblast growth factor and one of its high-affinity receptors (flg) following a localized cortical brain injury. J Neurosci 12:3828–3837PubMedGoogle Scholar
  27. MacGregor AJ, Dougherty AL, Galarneau MR (2011) Injury-specific correlates of combat-related traumatic brain injury in operation iraqi freedom. J Head Trauma Rehabil 26:312–318PubMedCrossRefGoogle Scholar
  28. Marshall LF (2000) Head injury: recent past, present, and future. Neurosurgery 47:546–561PubMedGoogle Scholar
  29. McGraw J, Hiebert GW, Steeves JD (2001) Modulating astrogliosis after neurotrauma. J Neurosci Res 63:109–115PubMedCrossRefGoogle Scholar
  30. Morris GF, Mathews MB (1989) Regulation of proliferating cell nuclear antigen during the cell cycle. J Biol Chem 264:13856–13864PubMedGoogle Scholar
  31. Nieto-Sampedro M (1999) Neurite outgrowth inhibitors in gliotic tissue. Adv Exp Med Biol 468:207–224PubMedCrossRefGoogle Scholar
  32. Norton WT (1999) Cell reactions following acute brain injury: a review. Neurochem Res 24:213–218PubMedCrossRefGoogle Scholar
  33. Percival JM, Thomas G, Cock TA, Gardiner EM, Jeffrey PL et al (2000) Sorting of tropomyosin isoforms in synchronised NIH 3T3 fibroblasts: evidence for distinct microfilament populations. Cell Motil Cytoskeleton 47:189–208PubMedCrossRefGoogle Scholar
  34. Perry SV (2001) Vertebrate tropomyosin: distribution, properties and function. J Muscle Res Cell Motil 22:5–49PubMedCrossRefGoogle Scholar
  35. Raghupathi R (2004) Cell death mechanisms following traumatic brain injury. Brain Pathol 14:215–222PubMedCrossRefGoogle Scholar
  36. Ridet JL, Malhotra SK, Privat A, Gage FH (1997) Reactive astrocytes: cellular and molecular cues to biological function. Trends Neurosci 20:570–577PubMedCrossRefGoogle Scholar
  37. Schevzov G, Gunning P, Jeffrey PL, Temm-Grove C, Helfman DM et al (1997) Tropomyosin localization reveals distinct populations of microfilaments in neurites and growth cones. Mol Cell Neurosci 8:439–454PubMedCrossRefGoogle Scholar
  38. Shi W, Gong P, Fan J, Yan YH, Ni L et al (2012) The expression pattern of ADP-ribosyltransferase 3 in rat traumatic brain injury. J Mol Histol 43:37–47PubMedCrossRefGoogle Scholar
  39. Sofroniew MV (2005) Reactive astrocytes in neural repair and protection. Neuroscientist 11:400–407PubMedCrossRefGoogle Scholar
  40. Stamm S, Casper D, Lees-Miller JP, Helfman DM (1993) Brain-specific tropomyosins TMBr-1 and TMBr-3 have distinct patterns of expression during development and in adult brain. Proc Natl Acad Sci USA 90:9857–9861PubMedCrossRefGoogle Scholar
  41. Vrhovski B, Lemckert F, Gunning P (2004) Modification of the tropomyosin isoform composition of actin filaments in the brain by deletion of an alternatively spliced exon. Neuropharmacology 47:684–693PubMedCrossRefGoogle Scholar
  42. Weinberger RP, Henke RC, Tolhurst O, Jeffrey PL, Gunning P (1993) Induction of neuron-specific tropomyosin mRNAs by nerve growth factor is dependent on morphological differentiation. J Cell Biol 120:205–215PubMedCrossRefGoogle Scholar
  43. Wong K, Wessels D, Krob SL, Matveia AR, Lin JL et al (2000) Forced expression of a dominant-negative chimeric tropomyosin causes abnormal motile behavior during cell division. Cell Motil Cytoskeleton 45:121–132PubMedCrossRefGoogle Scholar
  44. Xu T, Wang X, Cao M, Wu X, Yan Y et al (2012) Increased expression of BAG-1 in rat brain cortex after traumatic brain injury. J Mol Histol 43:335–342PubMedCrossRefGoogle Scholar
  45. Yan Y, Gong P, Jin W, Xu J, Wu X, et al. (2012) The cell-specific upregulation of bone morphogenetic protein-10 (BMP-10) in a model of rat cortical brain injury. J Mol Histol 43:543–552Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Xinmin Wu
    • 1
    • 2
  • Hongran Fu
    • 2
  • Feihui Zou
    • 2
  • Wei Jin
    • 2
  • Ting Xu
    • 2
  • Peipei Gong
    • 2
  • Jian Xu
    • 3
  • Yaohua Yan
    • 2
  • Gang Cui
    • 2
  • Kaifu Ke
    • 2
  • Yilu Gao
    • 2
  • Chunfeng Liu
    • 4
  • Yongjin Pan
    • 2
  1. 1.The Department of NeurologySecond Affiliated Hospital of Soochow UniversitySuzhouChina
  2. 2.Department of NeurologyAffiliated Hospital of Nantong UniversityNantongPeople’s Republic China
  3. 3.The Department of PsychiatryThe Fourth People’s Hospital of NantongNantongPeople’s Republic China
  4. 4.The Department of NeurologySecond Affiliated Hospital of Soochow UniversitySuzhouChina

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