Journal of Molecular Histology

, Volume 43, Issue 5, pp 565–569

Preconditioning with volatile anaesthetic sevoflurane in ischemic retinal lesion in rats

  • Krisztina Szabadfi
  • Bese Danyadi
  • Peter Kiss
  • Sridharan Manavalan
  • Robert Gabriel
  • Dora Reglodi
  • Andrea Tamas
  • Domonkos Trasy
  • Istvan Batai
Brief Communication

Abstract

Volatile anaesthetic agents have been recognized for their neuroprotective properties since the 1960s. However, little is known regarding the potential retinoprotective effects of preconditioning by anaesthetic drugs. Retinal ischemia can be modeled by permanent bilateral common carotid artery occlusion (BCCAO). Here we studied the degree of ischemic injury with preconditioning by sevoflurane in the rat retina. During the BCCAO operation and preconditioning Wistar rats were anaesthetized with 1 MAC of sevoflurane. The oxygen, carbon dioxide, and anaesthetic vapor concentration in the anaesthetizing box was monitored with a gas analyzer. We examined 4 groups: non- and preconditioning groups in control and BCCAO animals. The duration of preconditioning period was 1 h and it was performed 1 day before BCCAO. The retinas were processed for histological evaluation after 2 weeks survival to determine the cell number in the ganglion cell layer and the thickness of the whole retina and that of all retinal layers. BCCAO-induced retinal ischemic injury was ameliorated by sevoflurane preconditioning. Retinal thickness and the cell number in the ganglion cell layer were more retained in preconditioned animals after BCCAO compared to non-preconditioned group. These results suggest that preconditioning using sevoflurane could provide a new perspective in retinoprotective strategies.

Keywords

Retina Sevoflurane preconditioning Ischemia Retinoprotection 

References

  1. Adamcyk S, Robin E, Simerabet M, Kipnis E, Tavernier B, Vallet B, Bordet R, Lebuffe G (2010) Sevoflurane pre- and post-conditioning protect the brain via the mitochondrial KATP channel. Br J Anaesth 104:191–200CrossRefGoogle Scholar
  2. Atlasz T, Babai N, Reglodi D, Kiss P, Tamas A, Bari F, Domoki F, Gabriel R (2007) Diazoxide is protective in the rat retina against ischemic injury induced by bilateral carotid occlusion and glutamate-induced degeneration. Neurotox Res 12:105–111PubMedCrossRefGoogle Scholar
  3. Atlasz T, Szabadfi K, Kiss P, Racz B, Gallyas F, Tamas A, Gaal V, Marton Z, Gabriel R, Reglodi D (2010a) Pituitary adenylate cyclase activating polypeptide in the retina: focus on the retinoprotective effects. Ann NY Acad Sci 1200:128–139PubMedCrossRefGoogle Scholar
  4. Atlasz T, Szabadfi K, Kiss P, Tamas A, Toth G, Reglodi D, Gabriel R (2010b) Evaluation of the protective effects of PACAP with cell-specific markers in ischemia-induced retinal degeneration. Brain Res Bull 81:497–504PubMedCrossRefGoogle Scholar
  5. Beck-Schimmer B, Breitenstein S, Urech S, De Conno E, Wittlinger M, Puhan M, Jochum W, Spahn DR, Graf R, Clavien PA (2008) A randomized controlled trial on pharmacological preconditioning in liver surgery using a volatile anesthetic. Ann Surg 248:909–918PubMedCrossRefGoogle Scholar
  6. Biermann J, Lagreze WA, Dimitiu C, Stoykow C, Goebel U (2010) Preconditioning with inhalative carbon monoxide protects rat retinal ganglion cells from ischemia/reperfusion injury. Invest Ophthalmol Vis Sci 51:3784–3791PubMedCrossRefGoogle Scholar
  7. Brandt SK, Weatherly ME, Ware L, Linn DM, Linn CL (2011) Calcium preconditioning triggers neuroprotection in retinal ganglion cells. Neuroscience 172:387–397PubMedCrossRefGoogle Scholar
  8. Codaccioni JL, Velly LJ, Moubarik C, Bruder NJ, Pisano PS, Guillet BA (2009) Sevoflurane preconditioning against focal cerebral ischemia. Anesthesiology 110:1271–1278PubMedCrossRefGoogle Scholar
  9. Crawford MW, Lerman J, Pilato M, Orrego H, Saldivia V, Carmichael FJ (1992) Haemodynamic and organ blood flow responses to sevoflurane during spontaneous ventilation in the rat: a dose-response study. Can J Anaesth 39:270–276PubMedCrossRefGoogle Scholar
  10. Frässdorf J, Borowski A, Ebel D, Feindt P, Hermes M, Meemann T, Weber R, Müllenheim J, Weber NC, Preckel B, Schlack W (2009) Impact of preconditioning protocol on anesthetic-induced cardioprotection in patients having coronary artery bypass surgery. J Thorac Cardiovasc Surg 137:1436–1442PubMedCrossRefGoogle Scholar
  11. Kalenka A, Hinkelbein J, Feldmann RE, Kuschinsky W, Waschke KF, Maurer MH (2007) The effects of Sevoflurane anesthesia on rat brain proteins: a proteomic time-course analysis. Anesth Analg 104:1129–1135PubMedCrossRefGoogle Scholar
  12. Kaneko T, Yokoyama K, Makita K (2005) Late preconditioning with isoflurane in cultured rat cortical neurones. Br J Anaesth 95:662–668PubMedCrossRefGoogle Scholar
  13. Kapinya KJ, Löwl D, Fütterer C, Maurer M, Waschke KF, Isaev NK, Dirnagl U (2002) Tolerance against ischemic neuronal injury can be induced by volatile anesthetics and is inducible NO synthase dependent. Stroke 33:1889–1898PubMedCrossRefGoogle Scholar
  14. Kersten JR (2011) Anesthetic preconditioning. An anesthesiologist’s tale. Anesthesiology 114:162–166PubMedCrossRefGoogle Scholar
  15. Kersten JR, Schmeling TJ, Pagel PS, Gross GJ, Warltier DC (1997) Isoflurane mimics ischemic preconditioning via activation of K(ATP) channels: reduction of myocardial infarct size with an acute memory phase. Anesthesiology 87:361–370PubMedCrossRefGoogle Scholar
  16. Kitano H, Kirsch JR, Hurn PD, Murphy SJ (2007) Inhalational anesthetics as neuroprotectants or chemical preconditioning agents in ischemic brain. J Cereb Blood Flow Metab 27:1108–1128PubMedCrossRefGoogle Scholar
  17. Mester L, Szabo A, Atlasz T, Szabadfi K, Reglodi D, Kiss P, Racz B, Tamas A, Gallyas F Jr, Sumegi B, Hocsak E, Gabriel R, Kovacs K (2009) Protection against chronic hypoperfusion-induced retinal neurodegeneration by PARP inhibition via activation of PI3-kinase Akt pathway and suppression of JNK and p38 MAP kinases. Neurotox Res 18:68–76CrossRefGoogle Scholar
  18. Osborne NN, Casson RJ, Wood JP, Chidlow G, Graham M, Melena J (2004) Retinal ischemia: mechanisms of damage and potential therapeutic strategies. Prog Retinal Eye Res 23:91–147CrossRefGoogle Scholar
  19. Roth S (2009) Perioperative visual loss: what do we know, what can we do? Br J Anaesth 103(Suppl 1):i31–i40PubMedCrossRefGoogle Scholar
  20. Shen Y, Drum M, Roth S (2009) The prevalence of perioperative visual loss in the United States: a 10-year study from 1996 to 2005 of spinal, orthopedic, cardiac, and general surgery. Anesth Analg 109:1534–1545PubMedCrossRefGoogle Scholar
  21. Szabadfi K, Mester L, Reglodi D, Kiss P, Babai N, Racz B, Kovacs K, Szabo A, Tamas A, Gabriel R, Atlasz T (2010) Novel neuroprotective strategies in ischemic retinal lesions. Int J Mol Sci 11:544–561PubMedCrossRefGoogle Scholar
  22. Wang J, Meng F, Cottrell JE, Kass IS (2006) The differential effects of volatile anesthetics on electrophysiological and biochemical changes during and recovery after hypoxia in rat hippocampal slice CA1 pyramidal cells. Neuroscience 140(3):957–967PubMedCrossRefGoogle Scholar
  23. Wang R, Xu J, Xie J, Kang Z, Sun X, Chen N, Liu L, Xu J (2010a) Hypoerbaric oxygen preconditioning promotes survival of retinal ganglion cells in a rat model of optic nerve crush. J Neurotrauma 27:763–770PubMedCrossRefGoogle Scholar
  24. Wang S, Dai ZG, Dong XW, Guo SX, Liu Y, Wang ZP, Zeng YM (2010b) Duplicate preconditioning with sevoflurane in vitro improves neuroprotection in rat brain via activating the extracellular signal-regulated protein kinase. Neurosci Bull 26:437–444PubMedCrossRefGoogle Scholar
  25. Wang H, Lu S, Yu Q, Liang W, Gao H, Li P, Gan Y, Chen J, Gao Y (2011) Sevoflurane preconditioning confers neuroprotection via anti-inflammatory effects. Front Biosci 3:604–615CrossRefGoogle Scholar
  26. Xu X, Kim JA, Zuo Z (2008) Isoflurane preconditioning reduces mouse microglial activation and injury induced lipopolysaccharide and interferon-gamma. Neuroscience 154:1002–1008PubMedCrossRefGoogle Scholar
  27. Yang Q, Dong H, Deng J, Wang Q, Ye R, Li X, Hu S, Dong H, Xiong L (2011) Sevoflurane preconditioning induces neuroprotection through reactive oxygen species-mediated up-regulation of antioxidant enzymes in rats. Anesth Analg 112:931–937PubMedCrossRefGoogle Scholar
  28. Zhu Y, Zhang L, Gidday JM (2008) Deferroxamine preconditioning promotes long-lasting retinal ischemic tolerance. J Ocul Pharmacol Ther 24:527–535PubMedCrossRefGoogle Scholar
  29. Zhu W, Wang L, Zhang L, Palmateer JM, Libal NL, Hurn PD, Herson PS, Murphy SJ (2010) Isoflurane preconditioning neuroprotection in experimental focal stroke is androgen-dependent in male mice. Neuroscience 169:758–769PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Krisztina Szabadfi
    • 1
  • Bese Danyadi
    • 2
    • 3
  • Peter Kiss
    • 2
  • Sridharan Manavalan
    • 2
    • 4
  • Robert Gabriel
    • 1
  • Dora Reglodi
    • 2
  • Andrea Tamas
    • 2
  • Domonkos Trasy
    • 3
  • Istvan Batai
    • 3
  1. 1.Department of Experimental Zoology and Neurobiology, Institute of BiologyUniversity of PecsPecsHungary
  2. 2.Department of AnatomyPTE-MTA “Lendulet” PACAP Research TeamPecsHungary
  3. 3.Department of Anaesthesiology and Intensive CareUniversity of PecsPecsHungary
  4. 4.Department of Basic Sciences, College of Professional StudiesNational University of Health SciencesSt. PetersburgUSA

Personalised recommendations