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Journal of Molecular Histology

, Volume 43, Issue 5, pp 603–613 | Cite as

Protective effect of Panax ginseng against serum biochemical changes and apoptosis in kidney of rats treated with gentamicin sulphate

  • Yildiray Kalkan
  • Kubra Asena Terim Kapakin
  • Adem Kara
  • Tennur Atabay
  • Ali KaradenizEmail author
  • Nejdet Simsek
  • Emre Karakus
  • Ismail Can
  • Serap Yildirim
  • Seckin Ozkanlar
  • Emin Sengul
Original Paper

Abstract

The protective effects of Panax ginseng (PG) on gentamicin sulphate (GS) induced acute nephrotoxicity were investigated in rats. A total of 32 adult Sprague–Dawley rats were randomly divided into 4 equal groups and treated by intraperitoneous route for 10 days with: 0.5 mL of isotonic saline (group C), GS 100 mg/kg/day (group GS), co treatment PG (100 and 200 mg/kg/day) plus GS (100 mg/kg/day). After the last injection, kidney markers (urea, creatinine and blood urea nitrogen-BUN) and hepatic markers (aspartate aminotransferase-AST, alanine aminotransferase-ALT, gama glutamil transferase-GGT), and biochemical parameters were analyzed using diagnostic kits. Also, kidney changes were evaluated by immunohistochemical and stereological methods. GS treatment induced significant elevation (P < 0.05) in kidney and hepatic markers, most of biochemical parameters, and Bax immunoreactivity as well. However, co treatments with both doses of PG (100 and 200 mg/kg/day) significantly alleviated (P < 0.05) the GS-induced elevations and have partially protected rats from nephrotoxicity (reduction of kidney damage, and of urea, creatinine and BUN concentrations, and of apoptotic index). Both biochemical results and immunohistochemical evidence showed that administration of PG reduced the gentamicin-induced nephrotoxicity.

Keywords

Panax ginseng Gentamicin sulphate Nephrotoxicity Apoptosis 

Notes

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Yildiray Kalkan
    • 1
  • Kubra Asena Terim Kapakin
    • 2
  • Adem Kara
    • 3
  • Tennur Atabay
    • 4
  • Ali Karadeniz
    • 5
    Email author
  • Nejdet Simsek
    • 3
  • Emre Karakus
    • 6
  • Ismail Can
    • 3
  • Serap Yildirim
    • 7
  • Seckin Ozkanlar
    • 8
  • Emin Sengul
    • 5
  1. 1.Department of Histology and Embryology, Faculty of MedicineUniversity of RizeRizeTurkey
  2. 2.Department of Pathology, Faculty of Veterinary MedicineUniversity of AtatürkErzurumTurkey
  3. 3.Department of Histology and Embryology, Faculty of Veterinary MedicineUniversity of AtatürkErzurumTurkey
  4. 4.Bornova Veterinary Control and Research InstituteBornovaTurkey
  5. 5.Department of Physiology, Faculty of Veterinary MedicineUniversity of AtatürkErzurumTurkey
  6. 6.Department of Pharmacology and Toxicology, Faculty of Veterinary MedicineUniversity of AtatürkErzurumTurkey
  7. 7.Department of Physiology, Faculty of MedicineUniversity of AtatürkErzurumTurkey
  8. 8.Department of Biochemistry, Faculty of Veterinary MedicineUniversity of AtatürkErzurumTurkey

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