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Journal of Molecular Histology

, Volume 42, Issue 1, pp 3–13 | Cite as

Characterization of basal pseudopod-like processes in ileal and colonic PYY cells

  • Diego V. Bohórquez
  • Rashmi Chandra
  • Leigh Ann Samsa
  • Steven R. Vigna
  • Rodger A. LiddleEmail author
Original Paper

Abstract

The peptide tyrosine tyrosine (PYY) is produced and secreted from L cells of the gastrointestinal mucosa. To study the anatomy and function of PYY-secreting L cells, we developed a transgenic PYY-green fluorescent protein mouse model. PYY-containing cells exhibited green fluorescence under UV light and were immunoreactive to antibodies against PYY and GLP-1 (glucagon-like peptide-1, an incretin hormone also secreted by L cells). PYY-GFP cells from 15 μm thick sections were imaged using confocal laser scanning microscopy and three-dimensionally (3D) reconstructed. Results revealed unique details of the anatomical differences between ileal and colonic PYY-GFP cells. In ileal villi, the apical portion of PYY cells makes minimal contact with the lumen of the gut. Long pseudopod-like basal processes extend from these cells and form an interface between the mucosal epithelium and the lamina propria. Some basal processes are up to 50 μm in length. Multiple processes can be seen protruding from one cell and these often have a terminus resembling a synapse that appears to interact with neighboring cells. In colonic crypts, PYY-GFP cells adopt a spindle-like shape and weave in between epithelial cells, while maintaining contact with the lumen and lamina propria. In both tissues, cytoplasmic granules containing the hormones PYY and GLP-1 are confined to the base of the cell, often filling the basal process. The anatomical arrangement of these structures suggests a dual function as a dock for receptors to survey absorbed nutrients and as a launching platform for hormone secretion in a paracrine fashion.

Keywords

L cell Confocal microscopy Peptide YY Green fluorescent protein Pseudopod 

Notes

Acknowledgments

We thank Drs. Samuel Johnson and Yasheng Gao from the Light Microscopy Core Facility at Duke University for their valuable assistance with confocal microscopy. This work was supported by National Institutes of Health grant DK38626.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Diego V. Bohórquez
    • 1
    • 2
  • Rashmi Chandra
    • 1
    • 2
  • Leigh Ann Samsa
    • 1
    • 2
  • Steven R. Vigna
    • 1
    • 2
  • Rodger A. Liddle
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of MedicineDuke UniversityDurhamUSA
  2. 2.Veterans Affairs Medical CenterDurhamUSA
  3. 3.Duke University Medical CenterDurhamUSA

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