Tissue distribution and expression of paraoxonases and chemokines in mouse: the ubiquitous and joint localisation suggest a systemic and coordinated role
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A vicious cycle between oxidation and inflammation leads to complications in a growing number of disease states. Knowledge on tissue distribution of chemokines, mediators of inflammatory response, and paraoxonases, with antioxidant and anti-inflammatory actions, may be relevant. Using immunohistochemistry and quantitative real-time PCR we have investigated the distribution of PON1, 2 and 3, CCL2, 7, 8 and 12 and the chemokine receptor CCR2 protein and mRNA in 23 tissues from C57BL/6J mice. As expected, PON1, 2 and 3, CCL2, 7, 8 and 12 and CCR2 proteins were present in the vast majority of tissues investigated. Surprisingly, mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. The wide distribution and expression of mRNA for paraoxonases and CC-chemokines suggest a systemic, probably coordinated, role in the overall inflammatory response.
KeywordsCCR2 Chemokines Inflammation Monocyte-chemoattractant protein Paraoxonase
Supported by grants from the Instituto de Salud Carlos III (PI081175 and PI081381), Ministerio de Sanidad, Madrid, Spain. GA, AR, and RBD are recipients of post-graduate fellowships from the Generalitat de Catalunya (FI06/01054, SGR00503, and FI08/00064 respectively). FRS is a visiting scientist from the Facultad de Salud, Universidad Industrial de Santander, Bucaramanga, Colombia. Expert technical assistance was provided by Alba Folch and Núria Canela.
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