Journal of Molecular Histology

, Volume 41, Issue 2–3, pp 121–128 | Cite as

Antiapoptotic effect of l-carnitine on testicular irradiation in rats

  • Mehmet Kanter
  • Yeter Topcu-Tarladacalisir
  • Sule Parlar
Original Paper


We evaluated the effects of l-carnitine on apoptosis of germ cells in the rat testis following irradiation. Male Wistar rats were divided into three groups. Control group received sham irradiation plus physiological saline. Radiotherapy group received scrotal gamma-irradiation of 10 Gy as a single dose plus physiological saline. Radiotherapy + l-carnitine group received scrotal irradiation plus 200 mg/kg intraperitoneally l-carnitine. Twenty-four hours post-irradiation, the rats were sacrificed and testes were harvested. Testicular damage was examined by light and electron microscopy, and germ cell apoptosis was determined by terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate in situ nick end-labeling (TUNEL) technique. Morphologically, examination of irradiated testis revealed presence of disorganization and desquamation of germinal cells and the reduction in sperm count in seminiferous tubule lumen. Under electron microscopy, the morphological signs of apoptosis were frequently detected in spermatogonia. Apoptotic spermatogonia showed the marginal condensation of chromatin onto the nuclear lamina, nucleus and cytoplasm shrinkage and still functioning cell organelles. TUNEL-positive cells were significantly more numerous in irradiated rats than in control rats. l-carnitine treatment significantly attenuated the radiation-induced morphological changes and germ cell apoptosis in the irradiated rat testis. In conclusion, these results suggested that l-carnitine supplementation during the radiotherapy may be beneficial for spermatogenesis following testicular irradiation by decreasing germ cell apoptosis.


Irradiation l-Carnitine Testicular toxicity Germ cell apoptosis Rat 



The autors would like to thank to doctor Mustafa Cem Uzal for irradiation studies and doctor Fatma Nesrin Turan for statistical analysis.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Mehmet Kanter
    • 1
  • Yeter Topcu-Tarladacalisir
    • 1
  • Sule Parlar
    • 2
  1. 1.Department of Histology and Embryology, Faculty of MedicineTrakya UniversityEdirneTurkey
  2. 2.Department of Radiation Onchology, Faculty of MedicineTrakya UniversityEdirneTurkey

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