Journal of Molecular Histology

, Volume 40, Issue 2, pp 151–155 | Cite as

Expression of EGR-1 in a subset of olfactory bulb dopaminergic cells

  • Nami Akiba
  • Sunna Jo
  • Yosuke Akiba
  • Harriet Baker
  • John W. Cave
Brief Communication

Abstract

In the adrenal medulla, binding of the immediate early gene (IEG) proteins, EGR-1 (ZIF-268/KROX-24/NGFI-A) and AP-1, to the tyrosine hydroxylase (Th) proximal promoter mediate inducible Th expression. The current study investigated the potential role of EGR-1 in inducible Th expression in the olfactory bulb (OB) since IEGs bound to the AP-1 site in the Th proximal promoter are also necessary for activity-dependent OB TH expression. Immunohistochemical analysis of a naris-occluded mouse model of odor deprivation revealed weak EGR-1 expression levels in the OB glomerular layer that were activity-dependent. Immunofluorescence analysis indicated that a majority of glomerular cells expressing EGR-1 also co-expressed TH, but only small subset of TH-expressing cells contained EGR-1. By contrast, granule cells, which lack TH, exhibited EGR-1 expression levels that were unchanged by naris closure. Together, these finding suggest that EGR-1 mediates activity-dependent TH expression in a subset of OB dopaminergic neurons, and that there is differential regulation of EGR-1 in periglomerular and granule cells.

Keywords

Dopamine Olfactory bulb Immediate early gene Transcription Differentiation 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Nami Akiba
    • 1
  • Sunna Jo
    • 1
  • Yosuke Akiba
    • 1
  • Harriet Baker
    • 1
  • John W. Cave
    • 1
  1. 1.Department of Neurology and Neuroscience, Burke Medical Research InstituteWeill Cornell Medical CollegeWhite PlainsUSA

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