Expression of the ZNT (SLC30) family members in the epithelium of the mouse prostate during sexual maturation
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A prostate contains ~10-fold higher zinc than other soft organs. The function of the prostate is to produce a zinc-enriched seminal fluid. To establish a protein expression profile for zinc transporters involved in zinc efflux and intracellular sequestration/storage in the mouse prostate during sexual maturation, ZNT expression were investigated by immunohistochemistry. Our study demonstrated that ZNT proteins were differentially expressed in the prostate during sexual maturation. ZNT1 was mainly detected on the lateral membrane of the epithelium. Other ZNTs examined resided intracellularly. Among differences were a staining of ZNT2/ZNT5 in the ER-rich area of the epithelium in the anterior lobe, a staining of ZNT2 along the lateral and apical membrane, a luminal border staining of ZNT4, a staining of ZNT5 in the Golgi area of the epithelium in the ventral lobe, a uniform expression of ZNT6 across the lobes and ages, and a staining of ZNT7 in all lobes across ages.
KeywordsZinc transporters Slc30 family ZNT Mouse prostate Male genital tract Cellular zinc accumulation
We special thank Dr. Shannon L. Kelleher of the Pennsylvania State University for the αZNT2 antibody. We thank Dr. Alexander Borowsky of the Center for Comparative Medicine, UC Davis, for technical support in identification of prostatic lobes. This work was supported by the United States Department of Agriculture CRIS:5306-515-30-014-00D and the NIH grant P60MD00222.
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