Mesenchymal cells contribute to the synthesis and deposition of the laminin-5 γ2 chain in the invasive front of oral squamous cell carcinoma

  • Marcus Franz
  • Petra Richter
  • Christiane Geyer
  • Torsten Hansen
  • Lorena Dominguez Acuña
  • Peter Hyckel
  • Frank D. Böhmer
  • Hartwig Kosmehl
  • Alexander Berndt
Original Paper

Abstract

Tumour progression in oral squamous cell carcinoma (OSCC) is associated with a reorganisation of extracellular matrix. Laminin-5 (Ln-5) plays an important role for tumour migration and shows an increased expression in areas of direct tumour/stroma interactions. We have previously shown stromal spot like Ln-5/γ2 chain deposits distant from the basement membrane region. In this study we have analysed which cell type is responsible for Ln-5/γ2 chain synthesis in situ. Furthermore, we studied its spatial relation to TGF-β1 as well as the Ln-5 modulating enzymes matrix metalloproteinase (MMP) 2, membrane type-1 (MT1-) MMP and bone morphogenetic protein (BMP-) 1 by different techniques including triple immunofluorescence labelling and in situ hybridisation in OSCC. We found that the stromal spot-like Ln-5 deposits occurred in the invasive front in the vicinity of mesenchymal cells and vessel structures. In particular, not only carcinoma cells but also mesenchymal cells were shown to express the Ln-5/γ2 chain mRNA. Moreover, stromal Ln-5 deposits showed a spatial association with TGF-β1 as well as with MT1-MMP and BMP-1. Based on these findings we suggest that mesenchymal cells contribute to the promotion of tumour cell migration as well as vessel formation in OSCC by providing and organising promigratory Ln-5 fragments.

Keywords

Oral squamous cell carcinoma Extracellular matrix Laminin-5 TGF-β1 MMP2 MT1-MMP BMP-1 Immunofluorescence In situ hybridisation 

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Marcus Franz
    • 1
  • Petra Richter
    • 1
  • Christiane Geyer
    • 1
  • Torsten Hansen
    • 2
  • Lorena Dominguez Acuña
    • 3
  • Peter Hyckel
    • 4
  • Frank D. Böhmer
    • 5
  • Hartwig Kosmehl
    • 6
  • Alexander Berndt
    • 1
  1. 1.Institute of PathologyFriedrich Schiller UniversityJenaGermany
  2. 2.Institute of PathologyJohannes Gutenberg UniversityMainzGermany
  3. 3.Instituto de Recursos Naturales y AgrobiologiaSalamancaSpain
  4. 4.Clinic of Maxillofacial SurgeryFriedrich Schiller UniversityJenaGermany
  5. 5.Institute of Molecular Cell BiologyFriedrich Schiller UniversityJenaGermany
  6. 6.Institute of PathologyHELIOS-KlinikumErfurtGermany

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