Journal of Molecular Histology

, Volume 36, Issue 4, pp 257–263 | Cite as

Endometriotic Cells Exhibit Metaplastic Change and Oxidative DNA Damage as Well as Decreased Function, Compared to Normal Endometrium

Article

Summary

A widely accepted theory of the etiology of endometriosis is that it originates from the implantation and invasion of cells from retrograde menstruation to various sites in the body particularly the pelvic peritoneal cavity. Little is known of the function of these cells in ectopic sites. Normal endometrium was compared with endometriotic tissue using an antibody to Placental Cadherin (P Cadherin), a recently studied cadherin that is implicated in metaplasia and early neoplasia and also 8-hydroxyguanine, an indicator of oxidative DNA damage. Comparisons of endometrial tissue function were made using expression of transforming growth factor β-1 (TGFβ-1) and insulin-like growth factor-I (IGF-I). There was no labelling for anti-P Cadherin or anti-8-hydroxydeoxyguanosine in normal endometrium but marked labelling for both on the apical surface of the endometriotic epithelium. Studies of markers of normal endometrial function were all de-expressed in endometriosis. This study indicates that endometriosis cells are abnormal and exhibit oxidative DNA damage, metaplasia and markedly reduced function compared to normal endometrium.

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References

  1. Alvarez-Rodriguez, C, Baiza-Guzman, LA 1996Regulation of uterine cellular proliferation with estrogens and growth factorsGinecol Obstet Mex64406410PubMedGoogle Scholar
  2. Bush, M, Mele, J, Couchman, G, Walmer, D 1998Evidence of juxtacrine signaling for transforming growth factor alpha in human endometriumBiol Reprod5915221529PubMedGoogle Scholar
  3. Caniggia, I, Taylor, CV, Ritchie, JW, Lye, SJ, Letarte, M 1997Endoglin regulates trophoblast differentiation along the invasive pathway in human placental villous explantsEndocrinology13849774988CrossRefPubMedGoogle Scholar
  4. Chang, SY, Ho, YS 1997Immunohistochemical analysis of insulin-like growth factor I, insulin-like growth factor I receptor and insulin-like growth factor II in endometriotic tissue and endometriumActa Obstet Gynecol Scand76112117PubMedGoogle Scholar
  5. Chen, GT, Tai, CT, Yeh, LS, Yang, TC, Tsai, HD 2002Identification of the cadherin subtypes present in the human peritoneum and endometriotic lesions: potential role for P cadherin in the delopment of endometriosisMole Reprod Dev62289294CrossRefGoogle Scholar
  6. Chiou, CC, Chang, PY, Chan, EC, Wu, TL, Tsao, KC, Wu, JT 2003Urinary 8-hydroxydeoxyguanosine and its analogs as DNA markers of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancersClin Chim Acta3348794CrossRefPubMedGoogle Scholar
  7. Das, SK, Lim, H, Wang, J, Paria, BC, BazDresch, M, Dey, SK 1997Inappropriate expression of human transforming growth factor (TGF)-alpha in the uterus of transgenic mouse causes downregulation of TGF-beta receptors and delays the blastocyst-attachment reactionJ Mole Endocrinol18243257CrossRefGoogle Scholar
  8. Dore, JJ, Wilkinson, JE, Godkin, JD 1996Ovien endometrial expression of transforming growth factor beta isoforms during the peri-implantation periodBiol Reprod5410801087PubMedGoogle Scholar
  9. Fujimoto, J, Ichigo, S, Hori, M, Tamaya, T 1996Expression of E-cadherin, alpha- and beta-cadherin mRNAs in ovarian endometriosisEur J Obstet Gynecol Reprod Biol67179183CrossRefPubMedGoogle Scholar
  10. Furukawa, F, Fujii, K, Horiguchi, Y, Matsuyoshi, N, Fujita, M, Toda, K, Imamura, S, Wakita, H, Shirahama, S, Takigawa, M 1997Role of E- and P-cadherin in the human skinMicrosc Res Tech38343352CrossRefPubMedGoogle Scholar
  11. Gaetje, R, Kotzian, S, Herrmann, G, Baumann, R, Starzinski-Powitz, A 1997Nonmalignant epithelial cells, potentially invasive in human endometriosis, lack the tumor suppressor molecule E-cadherinAm J Pathol150461467PubMedGoogle Scholar
  12. Gu, Y, Branham, WS, Sheehan, DM, Webb, PJ, Moland, CL, Streck, RD 1999Tissue-specific expression of messenger ribonucleic acids for insulin-like growth factors and insulin-like growth factor-binding proteins during perinatal development of the rat uterusBiol Reprod6011721182PubMedGoogle Scholar
  13. Hansch, GM, Wagner, C, Burger, A, Dong, W, Staehler, G, Stoeck, M 1995Matrix protein synthesis by glomerular mesangial cells in culture: effects of transforming growth factor beta (TGF beta) and platelet-derived growth factor (PDGF) on fibronectin and collagen type IV mRNAJ Cell Physiol163451457CrossRefPubMedGoogle Scholar
  14. Hardy, RG, Brown, RM, Miller, SJ, Tselepis, C, Morton, DG, Jankowski, JA, Sanders, DS 2002Transient P-cadherin expression in radiation proctitis: a model of mucosal injury and repairJ Pathol197194200CrossRefPubMedGoogle Scholar
  15. Hardy, RG, Tselepis, C, Hoyland, J, Wallis, Y, Pretlow, TP, Talbot, I, Sanders, DS, Matthews, G, Morton, D, Jankowski, JA 2002Aberrant P-cadherin expression is an early event in hyperplastic and dysplastic transformation in the colonGut50513519CrossRefPubMedGoogle Scholar
  16. Li, D, Zhang, W, Sahin, AA, Hittelman, WN 1999DNA adducts in normal tissue adjacent to breast cancer: a reviewCancer Detect Prev23454462CrossRefPubMedGoogle Scholar
  17. Loverro, G, Maiorano, E, Napoli, A, Selvaggi, L, Marra, E, Perlino, E 2001Transforming growth factor-beta 1 and insulin-like growth factor-I expression in ovarian endometriotic cysts: a preliminary studyInt J Mole Med7423429Google Scholar
  18. Murdoch, WJ 2003Metaplastic potential of p53 down-regulation in ovarian surface epithelial cells affected by ovulationCancer Lett1917581CrossRefPubMedGoogle Scholar
  19. Nunomura, A 1999J Neurosci1919591968PubMedGoogle Scholar
  20. Parker, AR, O’Meally, RN, Oliver, DH, Hua, L, Nelson, WG, DeWeese, TL, Eshleman, JR 20028-Hydroxyguanosine repair is defective in some microsatellite stable colorectal cancer cellsCancer Res6272307233PubMedGoogle Scholar
  21. Paul, R, Ewing, CM, Jarrard, DF, Isaacs, WB 1997The cadherin cell–cell adhesion pathway in prostate cancer progressionBr J Urol793743PubMedGoogle Scholar
  22. Polli, V, Bulletti, C, Galassi, A, Borini, A, Ciotti, PM, Seracchioli, R, Alfieri, S, Flamigni, C 1996Transforming growth factor-beta 1 in the human endometriumGynecol Endocrinol10297302PubMedGoogle Scholar
  23. Robboy S, Anderson M, Russell P (Eds.) (2002) Pathology of the Female Reproductive Tract, Churchill Livingston, Sydney.Google Scholar
  24. Rosai J (Ed.) (1996) Ackerman’s Surgical Pathology, Mosby, New York.Google Scholar
  25. Sanders, DS, Perry, I, Hardy, R, Jankowski, J 2000Aberrant P-cadherin expression is a feature of clonal expansion in the gastrointestinal tract associated with repair and neoplasiaJ Pathol190526530CrossRefPubMedGoogle Scholar
  26. Sbracia, M, Zupi, E, Alo, P, Manna, C, Marconi, D, Scarpellini, F, Grasso, JA, Di Tondo, U, Romanini, C 1997Differential expression of IGF-I and IGF-II in eutopic and ectopic endometria of women with endometriosis and in women without endometriosisAm J Reprod Immunol37326329PubMedGoogle Scholar
  27. Scott, D, Sanders, A, Perry, I, Hardy, R, Jankowski, J 2000Aberrant P-cadherin expression is a feature of clonal expansion in the gastrointestinal tract associated with repair and neoplasiaJ Pathol190526530CrossRefPubMedGoogle Scholar
  28. Scotti, S, Regidor, PA, Schindler, AE, Winterhager, E 2000Reduced proliferation and cell adhesion in endometriosisMole Hum Reprod6610617CrossRefGoogle Scholar
  29. Slater, M 1999J Histotechnol221721Google Scholar
  30. Slater, M, Barden, JA, Murphy, CR 2000Histochem J32365372CrossRefPubMedGoogle Scholar
  31. Slater, M, Murphy, CR 1999aChondroitin sulphate and heparin sulfate proteoglycan are sequentially expressed in the uterine extracellular matrix during early pregnancy in the ratMatrix Biol18125131CrossRefGoogle Scholar
  32. Slater, M, Murphy, CR 1999bDifferential expression of insulin-like growth factors in the uterine epithelium and extracellular matrix during early pregnancyMatrix Biol18579584CrossRefGoogle Scholar
  33. Slater, M, Murphy, CR 1999cPremature implantation may be prevented by an inhibitory system regulated by epidermal growth factorActa Histochem101121126Google Scholar
  34. Slater, M, Murphy, CR 1999dThrombospondin is sequentially expressed and then de-expressed during early pregnancy in the rat uterusHistochem J31471475CrossRefGoogle Scholar
  35. Slater, M, Murphy, CR 2000Transforming growth factors alpha and beta-1 are co-expressed in the uterine epithelium during early pregnancyCell Tissue Res300315320Google Scholar
  36. Stern, RA, Dash, R, R, B 2001J Gynecol Pathol20133139CrossRefGoogle Scholar
  37. Takeichi, M 1993Cadherins in cancer: implications for invasion and metastasisCurr Opin Cell Biol5806811CrossRefPubMedGoogle Scholar
  38. Kleffens, M, Groffen, C, Lindenberghkortleve, DJ, Vanneck, JW, Gonzalezparra, S, Dits, N, Zwarthoff, EC, Drop, SLS 1998The IGF system during fetal–placental development of the mouseMole Cell Endocrinol140129135CrossRefGoogle Scholar
  39. Vleminckx, K, Vakaet, L, Mareel, M 1994Loss of membranous E-cadherin expression in pancreatic cancer: correlation with lymph node metastasis, high grade, and advanced stageJ Pathol174243248CrossRefPubMedGoogle Scholar
  40. Wang, YZ, Wong, YC 1998Sex hormone-induced prostatic carcinogenesis in the noble rat: the role of insulin-like growth factor I (IGF-I) and vascular endothelial growth factor (VEGF) in the development of prostate cancerProstate35165177CrossRefPubMedGoogle Scholar
  41. Xing, DY, Tan, W, Song, N, Lin, DX 2001Ser326Cys polymorphism in hOGG1 gene and risk of esophageal cancer in a Chinese populationInt J Cancer95140143CrossRefPubMedGoogle Scholar
  42. Zhang, X, Kidder, GM, Watson, AJ, Schultz, GA, Armstrong, DT 1994Possible roles of insulin and insulin-like growth factors in rat preimplantation development: investigation of gene expression by reverse transcription-polymerase chain reactionJ Reprod Fertil100375380PubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • M. Slater
    • 1
  • G. Quagliotto
    • 2
  • M Cooper
    • 3
  • C.R. Murphy
    • 1
  1. 1.Anatomy and Histology, School of Medical SciencesThe University of SydneyAustralia
  2. 2.Department of Anatomical PathologyRoyal Prince Alfred HospitalSydneyAustralia
  3. 3.Department of Endoscopic SurgeryKing George V HospitalSydneyAustralia

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