Journal of Molecular Histology

, Volume 36, Issue 1–2, pp 25–34 | Cite as

Endothelin-1 and endothelin receptors in the basilar artery of the capybara

  • Andrzej Loesch
  • Barbara Gajkowska
  • Michael R. Dashwood
  • Emerson T. Fioretto
  • Karina M. Gagliardo
  • Ana R. De Lima
  • Antonio A. C. M. Ribeiro
Article

Abstract

Little is known about cerebral vasculature of capybara, which seems may serve as a natural model of studying changes in cerebral circulation due to internal carotid artery atrophy at animal sexual maturation. This is the first study of the light- and electron-immunocytochemical localisation of endothelin-1 (ET-1) and ETA and ETB endothelin receptors in the basilar artery of capybaras (6 to 12-month-old females and males) using an ExtrAvidin detection method. All animals examined showed similar patterns of immunoreactivity. Immunoreactivity for ET-1 was detected in the endothelium and adventitial fibroblasts, whilst immunoreactivity for ETA and ETB receptors was present in the endothelium, vascular smooth muscle, perivascular nerves and fibroblasts. In endothelial cells immunoreactivity to ET-1 was pronounced in the cytoplasm or on the granular endoplasmic reticulum. Similar patterns of immunolabelling were observed for ETA and ETB receptors, though cytoplasmic location of clusters of immunoprecipitate seems dominant. These results suggest that the endothelin system is present throughout the wall of the basilar artery of capybara.

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Copyright information

© Springer 2005

Authors and Affiliations

  • Andrzej Loesch
    • 1
  • Barbara Gajkowska
    • 2
  • Michael R. Dashwood
    • 3
  • Emerson T. Fioretto
    • 4
  • Karina M. Gagliardo
    • 4
  • Ana R. De Lima
    • 4
  • Antonio A. C. M. Ribeiro
    • 4
  1. 1.Department of Anatomy and Developmental Biology (Royal Free Campus), Royal Free and University College Medical SchoolUniversity College LondonLondonUK
  2. 2.Department of Cell Ultrastructure, Medical Research CenterPolish Academy of SciencesWarsawPoland
  3. 3.Department of Clinical BiochemistryRoyal Free and University College Medical School (Royal Free Campus)LondonUK
  4. 4.Department of Surgery,College of Veterinary MedicineUniversity of São Paulo (USP)São Paulo-SPBrazil

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