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Mapping and validation of a major quantitative trait locus qRN5a associated with increasing root number under low potassium in rice

Abstract

Potassium (K) is an indispensable mineral constituent required for plant growth and many physiological processes. K deficiency and depletion in rice occurs frequently, resulting in limited growth, agronomical loss, and reduced yield. To investigate the genetics of low K (LK) tolerance, a set of high throughput genotyped chromosome segment substitution lines (CSSL) derived from the cross between Zhonghui9308 (ZH9308, susceptible to LK) and XieqingzaoB (tolerant to LK) was used to identify QTL for the shoot and root traits at the seedling stage. The experiment was conducted in hydroponic culture to explore the molecular basis of five seedling traits under two K conditions, LK and normal K (NK) and their ratio (LK/NK) for relative traits. A total of five QTL were identified on four chromosomes (3, 4, 5, and 6) with positive allelic effects from XieqingzaoB for root length (RL) and root number (RN) and negative allelic effects for shoot dry weight (SDW) and root dry weight (RDW). Two QTLs, qRN5a and qSDW4, were detected under LK and three QTLs, qRL6, qRN5b, and qRDW3, were identified under LK/NK ratio explaining 11.81% to 13.07% of total phenotypic variation. qRN5a, a novel QTL under LK, was validated in the F2 (BC5F2) population and delimited to a 1023 Kb interval between the markers InD78 to RM18472. These findings will serve as important breeding material for further genetic characterization like fine mapping and cloning, which may be useful in molecular marker-assisted breeding.

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Acknowledgements

This work was supported by the Zhejiang Provincial Natural Science Foundation of China (Grant number LQ14C130003), the National Natural Science Foundation of China (Grant numbers 31871604 and 31521064), the National Key Transform Program (Grant number 2016ZX08001-002), and the Super Rice Breeding Innovation Team and Rice Heterosis Mechanism Research Innovation Team of the Chinese Academy of Agricultural Sciences Innovation Project (Grant number CAAS-ASTIP-2013-CNRRI).

Author information

Conceptualization: AI, WW, and YZ; Methodology: AI, WW, and GA; Formal analysis and investigation: AI, MHR and WA; Writing—original draft preparation: AI; Writing—review and editing: AI, WW, YZ, GA, MHR, WA, XS, LC and SC; Funding acquisition: WW, LC and SC; Resources: YZ and XS; Supervision: WW, SC, and LC.

Correspondence to Liyong Cao or Shihua Cheng or Weixun Wu.

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Islam, A., Zhang, Y., Anis, G. et al. Mapping and validation of a major quantitative trait locus qRN5a associated with increasing root number under low potassium in rice. Plant Growth Regul (2020) doi:10.1007/s10725-020-00574-8

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Keywords

  • Rice
  • Chromosome segment substitution lines
  • Quantitative trait loci
  • Root number
  • Low potassium
  • Hydroponic culture