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Heterologous expression of the metallothionein PpMT2 gene from Physcomitrella patens confers enhanced tolerance to heavy metal stress on transgenic Arabidopsis plants

  • Yue LiuEmail author
  • Tao Kang
  • Jie-shan Cheng
  • Yan-jun Yi
  • Jun-jie Han
  • Hai-long Cheng
  • Qi Li
  • Na Tang
  • Mei-xia LiangEmail author
Original paper
  • 37 Downloads

Abstract

Metallothioneins (MTs) play essential roles in plant resistance to heavy metal stress, as well as in the scavenging of reactive oxygen species. A number of metallothionein genes in angiosperm have been identified. However, their functions in Physcomitrella patens are still largely unknown. In this work, the function of PpMT2, a metallothionein encoding gene from Physcomitrella patens, was investigated. Sequence alignment and phylogenetic analyses demonstrated that PpMT2 encoded a metallothionein with conserved sequences as other MTs from Arabidopsis, rice, soybean, black nightshade, sedum and poke weed. RT-PCR analyses revealed that PpMT2 was strongly induced by CuSO4 and CdCl2 in Physcomitrella patens. Heterologous expression of PpMT2 led to improved tolerance to high concentrations of CuSO4 and CdCl2 in both Y2HGold yeast cells and transgenic Arabidopsis plants. These findings suggest that PpMT2 is involved in heavy metal stress responses in Physcomitrella patens, and could be used as a potential candidate gene for the genetic engineering of plants with improved resistance to heavy metal stress.

Keywords

Arabidopsis Heavy metal stress Metallothionein Physcomitrella patens PpMT2 Transgenic plant 

Notes

Acknowledgements

We thank Prof. Yi-kun He (Capital Normal University, China) for providing Physcomitrella patens, and Ms. Jessie Zhang (Faculty of Health Sciences, McMaster University, Canada) for her critical reading and editing of this paper.

Funding

This work has been jointly supported by the following Grants: the National Nature Science Foundation of China (31872650); the Key R & D project of Shandong Province (2017NC210012, 2018GNC110007).

Compliance with ethical standards

Conflict of interest

We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

Supplementary material

10725_2019_558_MOESM1_ESM.docx (689 kb)
Supplementary material 1 (DOCX 689 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Yue Liu
    • 1
    • 4
    Email author
  • Tao Kang
    • 2
    • 4
  • Jie-shan Cheng
    • 2
    • 4
  • Yan-jun Yi
    • 3
  • Jun-jie Han
    • 5
  • Hai-long Cheng
    • 3
  • Qi Li
    • 3
  • Na Tang
    • 3
  • Mei-xia Liang
    • 2
    • 4
    Email author
  1. 1.College of Life SciencesQingdao UniversityQingdaoChina
  2. 2.College of AgricultureLudong UniversityYantaiChina
  3. 3.College of Life SciencesQingdao Agricultural UniversityQingdaoChina
  4. 4.Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic ResistantPlants in Universities of Shandong (Ludong University)YantaiChina
  5. 5.Yantai Academy of Agricultural SciencesYantaiChina

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