Overexpression of NHL6 affects seed production in transgenic Arabidopsis plants
- 65 Downloads
In higher plants, NHL (NDR1/HIN1-like) genes play important roles in response to both biotic and abiotic stresses. However, their biological functions in plant growth and development are largely unknown. Previously, we identified an NHL6 gene from Arabidopsis. Overexpression of NHL6 inhibited seed germination under abiotic stress conditions. In this work, we demonstrate that NHL6 also has a function in seed development. Transgenic Arabidopsis plants overexpressing NHL6 produced shorter siliques and fewer seeds per silique, but increased seed size, leading to decreased seed yield per plant. Pollen viability and meiosis studies did not show any difference between wild type and transgenic plants. In a yeast-two-hybrid screening, a tubby-like F-box protein AtTLP11 was identified as a potential interacting protein of NHL6, which was negatively correlated with NHL6 expression. Our results reveal a critical role of NHL gene in the seed development and a possible connection between NHL and AtTLP families in plants.
KeywordsArabidopsis Development NHL6 Seed production
This work was supported by the National Key Program on Transgenic Research [Grant No. 2018ZX08020002]; the National Key R & D Program of China [Grant No. 2016YFD0600106]; the National Natural Science Foundation of China [Grant No. 31870576]; the National Major Project of GMO New Species Cultivation [Grant No. 2016ZX08004-002-006]; The Modern Agricultural Industry Technology System Innovation Team of Shandong Province of China [Grant No. SDAIT-02-05]; and Comprehensive Surveys on Saline Lake Lithium and other New Energy Resources in the North Tibetan Plateau [Grant No. DD20160025].
Compliance with ethical standards
Conflict of interest
We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.
- Rasband WS, ImageJ US National Institutes of Health, Bethesda, Maryland, USA, https://imagej.nih.gov/ij/, 1997–2018
- Zheng MS, Takahashi H, Miyazaki A, Yamaguchi K, Kusano T (2005) Identification of the cis-acting elements in Arabidopsis thaliana NHL10 promoter responsible for leaf senescence, the hypersensitive response against Cucumber mosaic virus infection, and spermine treatment. Plant Sci 168:415–422CrossRefGoogle Scholar