UV-B response is modulated by cell-type specific signaling pathway in multicellular green algae Volvox carteri
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A fundamental question in biology is how multicellular organisms regulate cellular and physiological processes in response to environmental signals in a tissue/cell type-specific manner. Light is one such cue but little is known about its effect on molecular mechanisms underlying cell-type specific signaling. The Volvox genus presents a Germ-Soma differentiation that can be used to understand the genetic mechanisms of evolutionary transition from single-cell to multicellular organisms. Here we report the transcriptional analysis throughout both asexual and sexual life cycles of Volvox carteri in two different cell types under UV-B light irradiation. Our data show that VcUVR8-V1, the main splice variant of the VcUVR8 transcript, accumulates during initiation of cleavage division. Moreover, the transcript level of VcUVR8-V1 increases in response to the sex inducer. VcUVR8 expression seems to remain the same in both cell types, while VcCOP1, the interacting partner of VcUVR8, is expressed in a cell-type specific manner. Interestingly, illumination with low doses of UV-B leads to an increase of VcCOP1 transcript levels only in the somatic cells. Our results indicate that UV-B signaling pathway is differentially regulated between two cell types and environmental UV-B could be involved in cell-type specific regulation of developmental and physiological processes.
KeywordsUV-B photoreceptor UVR8 COP1 Gene expression Green algae Volvox carteri
This work has been supported by the Center for International Scientific Studies & Collaboration [CISSC (2013)] to JR and DFG Grant KI 1779/1-1 to AK. We would like thank Magdalena Dombrowa and Saskia Wöllner for their great experimental support during this study. Also, we thank Stefania Viola, Wenteng Xu and Girish Beedessee for reading of the manuscript and helpful suggestions.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Buchanan B, Gruissem W, Jones R (2000) Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Courier Companies. Inc, WaldorfGoogle Scholar
- Felsenstein J (1989) PHYLIP—phylogeny inference package (version 3.2). Cladistics 5, 164–166Google Scholar
- Hall TA (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41: 95–98Google Scholar
- Heijde M, Zabulon G, Corellou F, Ishikawa T, Brazard J, Usman A, Sanchez F, Plaza P, Martin M, Falciatore A, Todo T, Bouget FY, Bowler C (2010) Characterization of two members of the cryptochrome/photolyase family from Ostreococcus tauri provides insights into the origin and evolution of cryptochromes. Plant Cell Environ 33:1614–1626CrossRefGoogle Scholar
- Kianianmomeni A (2015a) Cell-type specific photoreceptors and light signaling pathways in the multicellular green alga Volvox carteri and their potential role in cellular differentiation. Plant Signal Behav 10: e1010935-1- e1010935-4Google Scholar
- Kianianmomeni A (2015b) Potential impact of gene regulatory mechanisms on the evolution of multicellularity in the volvocine algae. Commun Integr Biol 8: e1017175-1- e1017175-4Google Scholar
- Kianianmomeni A, Hallmann A (2014b) Transcriptional analysis of Volvox photoreceptors suggests the existence of different cell-type specific light-signaling pathways. Curr Genet 61:1–16Google Scholar
- Kirk DL (1998) Volvox: molecular-genetic origins of multicellularity and cellular differentiation. Cambridge University Press, CambridgeGoogle Scholar
- Lumsden P (1997) Plants and UV-B: responses to environmental change. Cambridge University Press, CambridgeGoogle Scholar
- Prochnik SE, Umen J, Nedelcu AM, Hallmann A, Miller SM, Nishii I, Ferris P, Kuo A, Mitros T, Fritz-Laylin LK, Hellsten U, Chapman J, Simakov O, Rensing SA, Terry A, Pangilinan J, Kapitonov V, Jurka J, Salamov A, Shapiro H, Schmutz J, Grimwood J, Lindquist E, Lucas S, Grigoriev IV, Schmitt R, Kirk D, Rokhsar DS (2010) Genomic analysis of organismal complexity in the multicellular green alga Volvox carteri. Science 329:223–226CrossRefGoogle Scholar
- Provasoli L, Pintner IJ (1959) Artificial media for fresh-water algae: problems and suggestions. In Tryon CA, Hartman RT (eds) The ecology of algae, a symposium held at the pymatuning laboratory of field biology on June 18 and 19. The Pymatuning Symposia in Ecology, Special Publication No 2, University of Pittsburgh, Pittsburgh 84–96Google Scholar
- Starr RC (1969) Structure, reproduction and differentiation in Volvox carteri f. nagariensis Lyengre strains HK9 and 10. Arch Protistenkd Bd 111:204–222Google Scholar