Transcriptome profiles reveal new regulatory factors of anthocyanin accumulation in a novel purple-colored cherry tomato cultivar Jinling Moyu
- 18 Downloads
Anthocyanins are important secondary metabolites with high nutraceutical value. They represent the major red, purple, violet and blue pigments in higher plants but are usually not present in the fruits of domesticated tomatoes. Recently, several genes that regulate anthocyanin accumulation have been identified in wild tomatoes or mutation lines; however, whether there are other new factors involved in the regulation of anthocyanin biosynthesis is still unknown. “Jinling Moyu” (MY) is a novel purple cherry tomato that accumulates considerable anthocyanins in its ripe fruit. A transcriptome comparison analysis of MY fruits with pink and green fruit revealed that several structural genes assigned to the anthocyanin biosynthetic pathway were highly expressed in MY tomato, but all six known regulatory genes of anthocyanin accumulation in tomato fruits were rarely expressed among the samples in this study. However, four other R2R3–MYB and five other bHLH transcription factors were highly correlated with the differentially expressed structural genes in the anthocyanin pathway, indicating that these transcription factors might regulate anthocyanin biosynthesis in MY tomato. Our study will aid in understanding the complex mechanism regulating anthocyanin pigmentation in tomato fruits .
KeywordsTomato Purple fruit Anthocyanin Transcription factor
We thank Drs. Jinhua Li and Yong Xue for their critical review of this manuscript. We also thank Springer-Nature Author Service for English Language Editing. This work was financially supported by the National Key Research and Development Plan [Grant No. 2016YFD0101703], the National Natural Science Foundation of China (Grant No. 31501763 and 31501022), Natural Science Foundation of Jiangsu Province, China (Grant No. BK20140440 and BK20140443) and the Jiangsu Agricultural Independent Innovation Foundation of China [Grant No. CX(14)5084].
YW designed the research, collected the samples and performed the anthocyanin extraction and quantification. ZL collected the samples, and performed the RNA isolation and qRT-PCR experiments. CL analyzed the RNA-Seq data and helped in manuscript writing. RZ and LZ helped in collecting the samples. HZ helped in data analysis. WY contributed to concept the idea. TZ designed the research and wrote the manuscript. All authors have read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interest.
- Huang ZJ, Du YC, Qiu ZK, Cao X, Wang XY, Gao JC, Guo YM (2016) Molecular markers closely linked to tomato purple black fruit gene ATV and uses thereof. China Patent 201610405970.9Google Scholar
- Mes PJ, Boches P, Myers JR, Durst R (2008) Characterization of tomatoes expressing anthocyanin in the fruit. J Am Soc Hortic Sci 133(2):262–269Google Scholar