Plant Growth Regulation

, Volume 86, Issue 3, pp 433–444 | Cite as

Regulation of growth, nutritive, phytochemical and antioxidant potential of cultivated Drimiopsis maculata in response to biostimulant (vermicompost leachate, VCL) application

  • Lister Dube
  • Kuben K. Naidoo
  • Georgina D. Arthur
  • Adeyemi O. AremuEmail author
  • Jiri Gruz
  • Michaela Šubrtová
  • Monika Jarošová
  • Petr Tarkowski
  • Karel Doležal
Original Paper


The effect of vermicompost leachate (VCL, low-cost biostimulant) on the growth, elemental (macro and micro-nutrients) and phytochemical content as well as the antioxidant potential of Drimiopsis maculata was evaluated. Three dilutions (1:5; 1:10 and 1:20) of VCL were tested and the cultivation lasted for 3 months. In addition to the recorded growth parameters, dried and ground plant materials (leaves and bulbs) were evaluated for nutrients, phenolic acids and antioxidant capacity. Vermicompost leachate application enhanced the growth of D. maculata, particularly, the leaves (VCL 1:10) and bulbs (VCL 1:20) which were significantly bigger than the controls. Apart from the concentration of phosphorus which was significantly lower in the leaves of VCL (1:20)-treated plants, the quantity of all four macro-nutrients analysed were similar with and without VCL. Similar observations were also demonstrated in the majority of quantified micro-nutrients in D. maculata. Relative to the control, VCL-treated plants had higher concentrations of the 10 phenolic acids quantified in the leaves. However, the majority of the quantified phenolic acids were not significantly enhanced in bulbs. Antioxidant activity of D. maculata extracts was generally higher in leaves than in the bulbs. The leaf extract from VCL (1:10 and 1:20)-treated plants exhibited lower oxygen radical absorbance capacity (ORAC) when compared to the control. However, bulbs from VCL (1:5) treatment had significantly higher ORAC than the control. From a conservational perspective, the current findings provided insight on viable approaches useful for mitigating challenges associated with over-harvesting of highly utilized but slow-growing plant species.


Asparagaceae Conservation Geophytes Phenolic acids Plant nutrients Medicinal plants 



2,2-Azobis(2-methylpropionamidine) dihydrochloride


Completely randomised design


Dry weight


Inductively coupled plasma mass spectrometry


Oxygen radical absorbance capacity


Octapole reaction system


Trolox equivalents


Ultra-high performance liquid chromatography


Vermicompost leachate



This work was financially supported by Mangosuthu University of Technology under registered project NSci\05\2012 (LD, KKN, GDA), National Research Foundation (Incentive Funding for Rated Researchers, UID: 109508) and Faculty Research Committee, Faculty of Natural and Agricultural Sciences, North-West University, Mmabatho, South Africa (AOA). MJ, JG, PT and KD were supported by grant No. LO1204 (Sustainable development of research in Centre of Region Haná) from the National Program of Sustainability I, MEYS, Czech Republic. JG was also supported by the Czech Science Foundation (No. 17-06613S). KD was also supported by MEYS of CR from European Regional Development Fund-Project Centre for Experimental Plant Biology: No. CZ.02.1.01/0.0/0.0/16_019/0000738. We thank the staff and management of Silverglen Nature Reserve for supplying the bulbs used for the study.

Author contributions

LD, KKN, GDA and AOA conceived and conducted the field experiment. MJ and JG quantified the phenolic acids and ORAC assay. MJ and PT conducted the elemental analysis. LD, KKN, GDA and AOA analysed the data on growth parameters. KD was also involved in conceptualization, design and provided technical and editorial inputs. AOA wrote the manuscript with help from all the other authors.


  1. Affolter JM, Pengelly A (2007) Conserving medicinal plant biodiversity. In: Wynn SG, Fougère BJ (eds) Veterinary herbal medicine. Mosby, Saint Louis, pp 257–263CrossRefGoogle Scholar
  2. Aremu AO, Masondo NA, Van Staden J (2014) Physiological and phytochemical responses of three nutrient-stressed bulbous plants subjected to vermicompost leachate treatment. Acta Physiol Plant 36:721–731CrossRefGoogle Scholar
  3. Aremu AO, Masondo NA, Rengasamy KRR, Amoo SO, Gruz J, Bíba O, Šubrtová M, Pěnčík A, Novák O, Doležal K, Van Staden J (2015a) Physiological role of phenolic biostimulants isolated from brown seaweed Ecklonia maxima on plant growth and development. Planta 241:1313–1324CrossRefGoogle Scholar
  4. Aremu AO, Stirk WA, Kulkarni MG, Tarkowská D, Turečková V, Gruz J, Šubrtová M, Pěnčík A, Novák O, Doležal K, Strnad M, Van Staden J (2015b) Evidence of phytohormones and phenolic acids variability in garden-waste-derived vermicompost leachate, a well-known plant growth stimulant. Plant Growth Regul 75:483–492CrossRefGoogle Scholar
  5. Aremu AO, Plačková L, Gruz J, Bíba O, Novák O, Stirk WA, Doležal K, Van Staden J (2016) Seaweed-derived biostimulant (Kelpak®) influences endogenous cytokinins and bioactive compounds in hydroponically grown Eucomis autumnalis. J Plant Growth Regul 35:151–162CrossRefGoogle Scholar
  6. Arthur GD, Aremu AO, Kulkarni MG, Van Staden J (2012) Vermicompost leachate alleviates deficiency of phosphorus and potassium in tomato seedlings. HortScience 47:1304–1307Google Scholar
  7. Asami DK, Hong Y-J, Barrett DM, Mitchell AE (2003) Comparison of the total phenolic and ascorbic acid content of freeze-dried and air-dried marionberry, strawberry, and corn grown using conventional, organic, and sustainable agricultural practices. J Agric Food Chem 51:1237–1241CrossRefGoogle Scholar
  8. Brown P, Saa S (2015) Biostimulants in agriculture. Front Plant Sci. CrossRefPubMedPubMedCentralGoogle Scholar
  9. Calvo P, Nelson L, Kloepper JW (2014) Agricultural uses of plant biostimulants. Plant Soil 383:3–41CrossRefGoogle Scholar
  10. Canter PH, Thomas H, Ernst E (2005) Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends Biotechnol 23:180–185CrossRefGoogle Scholar
  11. Craigie JS (2011) Seaweed extract stimuli in plant science and agriculture. J Appl Phycol 23:371–393CrossRefGoogle Scholar
  12. Dold AP, Cocks ML (2002) The trade in medicinal plants in the Eastern Cape Province, South Africa. S Afr J Sci 98:589–597Google Scholar
  13. du Toit K, Elgorashi EE, Malan SF, Drewes SE, van Staden J, Crouch NR, Mulholland DA (2005) Anti-inflammatory activity and QSAR studies of compounds isolated from Hyacinthaceae species and Tachiadenus longiflorus Griseb. (Gentianaceae). Bioorg Med Chem 13:2561–2568CrossRefGoogle Scholar
  14. Gruz J, Novák O, Strnad M (2008) Rapid analysis of phenolic acids in beverages by UPLC–MS/MS. Food Chem 111:789–794CrossRefGoogle Scholar
  15. Gülçin İ (2012) Antioxidant activity of food constituents: an overview. Arch Toxicol 86:345–391CrossRefGoogle Scholar
  16. Halpern M, Bar-Tal A, Ofek M, Minz D, Muller T, Yermiyahu U (2015) The use of biostimulants for enhancing nutrient uptake. In: Sparks DL (ed) Advances in agronomy, vol 130. Academic Press, Cambridge, pp 141–174Google Scholar
  17. Heleno SA, Martins A, Queiroz MJRP, Ferreira ICFR (2015) Bioactivity of phenolic acids: metabolites versus parent compounds: a review. Food Chem 173:501–513CrossRefGoogle Scholar
  18. Hulme M (1954) Wild flowers of Natal. Shuter & Shooter, PietermaritzburgGoogle Scholar
  19. Hutchings A, Scott AH, Lewis G, Cunningham A (1996) Zulu medicinal plants. An Inventory. University of Natal Press, PietermaritzburgGoogle Scholar
  20. Ievinsh G (2011) Vermicompost treatment differentially affects seed germination, seedling growth and physiological status of vegetable crop species. Plant Growth Regul 65:169–181CrossRefGoogle Scholar
  21. Koorbanally C, Crouch NR, Mulholland DA (2001) Scillascillin-type homoisoflavanones from Drimiopsis maculata (Hyacinthaceae). Biochem Syst Ecol 29:539–541CrossRefGoogle Scholar
  22. Koorbanally C, Mulholland DA, Crouch NR (2006) Norlignans and homoisoflavanones from two South African Drimiopsis species (Hyacinthaceae: Hyacinthoideae). Biochem Syst Ecol 34:588–592CrossRefGoogle Scholar
  23. Kulkarni Y, Warhade KK, Bahekar S (2014) Primary nutrients determination in the soil using UV spectroscopy. Int J Emerg Eng Res Technol 2:198–204Google Scholar
  24. Kumar N, Pruthi V (2014) Potential applications of ferulic acid from natural sources. Biotechnol Rep 4:86–93CrossRefGoogle Scholar
  25. Lubbe A, Verpoorte R (2011) Cultivation of medicinal and aromatic plants for specialty industrial materials. Ind Crops Prod 34:785–801CrossRefGoogle Scholar
  26. Lyson TA (2002) Advanced agricultural biotechnologies and sustainable agriculture. Trends Biotechnol 20:193–196CrossRefGoogle Scholar
  27. Manning JC, Goldblatt P, Fay MF (2004) A revised generic synopsis of Hyacinthaceae in sub-Saharan Africa, based on molecular evidence, including new combinations and the new tribe Pseudoprospereae. Edinb J Bot 60:533–568Google Scholar
  28. Martínez-Ballesta MC, Dominguez-Perles R, Moreno DA, Muries B, Alcaraz-López C, Bastías E, García-Viguera C, Carvajal M (2010) Minerals in plant food: effect of agricultural practices and role in human health. A review. Agron Sustain Dev 30:295–309CrossRefGoogle Scholar
  29. Masondo NA, Kulkarni MG, Rengasamy KRR, Pendota SC, Finnie JF, Van Staden J (2016) Effect of vermicompost leachate in Ceratotheca triloba under nutrient deficiency. Acta Physiol Plant 38:236. CrossRefGoogle Scholar
  30. Moyo M, Aremu AO, Van Staden J (2015) Medicinal plants: an invaluable, dwindling resource in sub-Saharan Africa. J Ethnopharmacol 174:595–606CrossRefGoogle Scholar
  31. Mulholland DA, Koorbanally C, Crouch NR, Sandor P (2004) Xanthones from Drimiopsis maculata. J Nat Prod 67:1726–1728CrossRefGoogle Scholar
  32. Ou B, Hampsch-Woodill M, Prior RL (2001) Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Agric Food Chem 49:4619–4626CrossRefGoogle Scholar
  33. Pant AP, Radovich TJK, Hue NV, Talcott ST, Krenek KA (2009) Vermicompost extracts influence growth, mineral nutrients, phytonutrients and antioxidant activity in pak choi (Brassica rapa cv. Bonsai, Chinensis group) grown under vermicompost and chemical fertiliser. J Sci Food Agric 89:2383–2392CrossRefGoogle Scholar
  34. Pant AP, Radovich TJK, Hue NV, Paull RE (2012) Biochemical properties of compost tea associated with compost quality and effects on pak choi growth. Sci Hortic 148:138–146CrossRefGoogle Scholar
  35. Pham-Huy LA, He H, Pham-Huy C (2008) Free radicals, antioxidants in disease and health. Int J Biomed Sci 4:89–96PubMedPubMedCentralGoogle Scholar
  36. Reinten EY, Coetzee JH, van Wyk B-E (2011) The potential of South African indigenous plants for the international cut flower trade. S Afr J Bot 77:934–946CrossRefGoogle Scholar
  37. Rimmer DL (2006) Free radicals, antioxidants, and soil organic matter recalcitrance. Eur J Soil Sci 57:91–94CrossRefGoogle Scholar
  38. Sharma HSS, Fleming C, Selby C, Rao JR, Martin T (2014) Plant biostimulants: a review on the processing of macroalgae and use of extracts for crop management to reduce abiotic and biotic stresses. J Appl Phycol 26:465–490CrossRefGoogle Scholar
  39. Spector A (2000) Oxidative stress and disease. J Ocul Pharmacol Ther 16:193–201CrossRefGoogle Scholar
  40. Tilman D, Cassman KG, Matson PA, Naylor R, Polasky S (2002) Agricultural sustainability and intensive production practices. Nature 418:671–677CrossRefGoogle Scholar
  41. Toor RK, Savage GP, Heeb A (2006) Influence of different types of fertilisers on the major antioxidant components of tomatoes. J Food Compos Anal 19:20–27CrossRefGoogle Scholar
  42. Wang D, Shi Q, Wang X, Wei M, Hu J, Liu J, Yang F (2010) Influence of cow manure vermicompost on the growth, metabolite contents, and antioxidant activities of Chinese cabbage (Brassica campestris ssp. chinensis). Biol Fertil Soils 46:689–696CrossRefGoogle Scholar
  43. Wang K-H, Radovich T, Pant A, Cheng Z (2014) Integration of cover crops and vermicompost tea for soil and plant health management in a short-term vegetable cropping system. Appl Soil Ecol 82:26–37CrossRefGoogle Scholar
  44. Wiersum KF, Dold AP, Husselman M, Cocks M (2006) Cultivation of medicinal plants as a tool for biodiversity conservation and poverty alleviation in the Amatola region, South Africa. In: Bogers RJ, Craker LE, Lange D (eds) Medicinal and aromatic plants. Springer, Dordrecht, pp 43–57CrossRefGoogle Scholar
  45. Williams VL, Balkwill K, Witkowski ETF (2007) Size-class prevalence of bulbous and perennial herbs sold in the Johannesburg medicinal plant markets between 1995 and 2001. S Afr J Bot 73:144–155CrossRefGoogle Scholar
  46. Yakhin OI, Lubyanov AA, Yakhin IA, Brown PH (2017) Biostimulants in plant science: a global perspective. Front Plant Sci 7:
  47. Zhang H, Tan SN, Wong WS, Ng CYL, Teo CH, Ge L, Chen X, Yong JWH (2014) Mass spectrometric evidence for the occurrence of plant growth promoting cytokinins in vermicompost tea. Biol Fert Soils 50:401–403CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Lister Dube
    • 1
  • Kuben K. Naidoo
    • 1
  • Georgina D. Arthur
    • 1
  • Adeyemi O. Aremu
    • 2
    • 3
    Email author
  • Jiri Gruz
    • 4
  • Michaela Šubrtová
    • 4
  • Monika Jarošová
    • 5
  • Petr Tarkowski
    • 5
    • 6
  • Karel Doležal
    • 4
  1. 1.Department of Nature Conservation, Faculty of Natural SciencesMangosuthu University of TechnologyDurbanSouth Africa
  2. 2.Indigenous Knowledge Systems (IKS) Centre, Faculty of Natural and Agricultural SciencesNorth-West UniversityMmabathoSouth Africa
  3. 3.Food Security and Safety Niche Area, Faculty of Natural and Agricultural SciencesNorth West UniversityMmabathoSouth Africa
  4. 4.Laboratory of Growth Regulators & Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of SciencePalacký University & Institute of Experimental Botany AS CROlomoucCzech Republic
  5. 5.Centre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of SciencePalacký UniversityOlomoucCzech Republic
  6. 6.Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special PlantsCrop Research InstituteOlomoucCzech Republic

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