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Plant Growth Regulation

, Volume 86, Issue 3, pp 333–338 | Cite as

Phenolic and flavonoid production and antimicrobial activity of Gymnosporia buxifolia (L.) Szyszyl cell cultures

  • Aloka Kumari
  • Devashan Naidoo
  • Ponnusamy Baskaran
  • Karel Doležal
  • Jaroslav Nisler
  • Johannes Van StadenEmail author
Brief communication
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Abstract

Gymnosporia buxifolia (Celastraceae) is a well-known traditional medicinal plant used to treat various diseases. The aim of the study was to quantify the total phenolic and flavonoid content of cell biomass of G. buxifolia developed in vitro using plant growth regulators (PGRs), phloroglucinol (PG) and an antagonist of cytokinin activity 6-(2-hydroxy-3-methylbenzylamino) purine (PI55). The antibacterial activity of calli was also evaluated. The accumulation of phenolic contents and its antibacterial activity in the cell biomass varied between the treatments as well as the mother plant. Generally, a higher accumulation of phenolic contents translated to improved activity against selected pathogenic bacteria. This was apparent in biomass derived from solid and liquid MS media containing combinations of 5 µM PG, 1.5 µM benzyladenine (BA) or meta-topolin (mT) with or without 1 µM picloram (Pic) and 5 µM PG or PI55, 1 µM BA with or without 0.5 µM Pic respectively. The choice of PGRs, PG and PI55 treatments used during in vitro cell culture systems influenced the therapeutic potential of G. buxifolia. Our results indicate that the cell biomass from suspension and/or solid culture of G. buxifolia could be promising as antibacterial agents with possible applications in the pharmaceutical industry.

Keywords

Antibacterial activity Biomass Medicinal plant Phenolic content Plant cell culture 

Notes

Acknowledgements

The financial support by the University of KwaZulu-Natal, Pietermaritzburg, as well as the Ministry of Education, Youth and Sports, Czech Republic (Grant LO1204 from the National Program of Sustainability I.) is gratefully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

References

  1. Banasiuk R, Kawiak A, Krolicka A (2012) In vitro cultures of carnivorous plants from the Drosera and Dionaea genus for the production of biologically active secondary metabolites. BioTechnologia 93:87–96CrossRefGoogle Scholar
  2. Baskaran P, Ncube B, Van Staden J (2012) In vitro propagation and secondary product production by Merwilla plumbea (Lindl.) Speta. Plant Growth Regul 67:235–245CrossRefGoogle Scholar
  3. Baskaran P, Singh S, Van Staden J (2013) In vitro propagation, proscillaridin A production and antibacterial activity in Drimia robusta. Plant Cell Tiss Org Cult 114:259–267CrossRefGoogle Scholar
  4. Baskaran P, Kumari A, Naidoo D, Van Staden J (2015) In vitro propagation and biochemical changes in Aloe pruinosa. Ind Crops Prod 77:51–58CrossRefGoogle Scholar
  5. Boon R (2010) Pooley’s trees of eastern South Africa. Flora and Fauna Publications Trust, DurbanGoogle Scholar
  6. Bosch CH (2004) Maytenus heterophylla (Eckl. & Zeyh.) N. Robson. http://darabase.prota.org/PROTAhtmi/Mavtenus%20heterophylla En.htm
  7. Ceasar SA, Maxwell SL, Prasad KB, Karthigan M, Ignacimuthu S (2010) Highly efficient shoot regeneration of Bacopa monnieri (L.) using a two-stage culture procedure and assessment of genetic integrity of micropropagated plants by RAPD. Acta Physiol Plant 32:443–452CrossRefGoogle Scholar
  8. Collin HA (2001) Secondary product formation in tissue cultures. Plant Growth Regul 34:119–134CrossRefGoogle Scholar
  9. Di Carlo G, Mascolo N, Izzo AA, Capasso F (1999) Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sci 65:337–353CrossRefGoogle Scholar
  10. El-Bakry AA, Mostafa HAM, Alam Eman A (2014) Antioxidant and antibacterial activity of callus and adventitious root extracts from Rumex vesicarius L. J Med Plant Res 8:479–488CrossRefGoogle Scholar
  11. Eloff JN (1998) A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med 29:129–132Google Scholar
  12. García-Pérez E, Gutiérrez-Uribe JA, García-Lara S (2012) Luteolin content and antioxidant activity in micropropagated plants of Poliomintha glabrescens (Gray). Plant Cell Tissue Cult 108:521–527CrossRefGoogle Scholar
  13. Hutchings A, Scott AH, Lewis G, Cunningham AB (1996) Zulu medicinal plants: an inventory. University of Natal Press, Pietermaritzburg, pp 38–44Google Scholar
  14. Killian C (2009) Antioxidant properties of Gymnosporia buxifolia Szyszyl. Thesis, pp. 66–92Google Scholar
  15. Kumari A, Baskaran P, Van Staden J (2015) Enhanced HIV-1 reverse transcriptase inhibitory and antibacterial properties in callus of Catha edulis Forsk. Phytother Res 29:840–843CrossRefGoogle Scholar
  16. Kumari A, Baskaran P, Van Staden J (2016) In vitro propagation and antibacterial activity in Cotyledon orbiculata: a valuable medicinal plant. Plant Cell Tiss Org Cult 124:97–104CrossRefGoogle Scholar
  17. Liao DY, Chai YC, Wang SH, Chen CW, Tsai MS (2015) Antioxidant activities and contents of flavonoids and phenolic acids of Talinum triangulare extracts and their immunomodulatory effects. J Food Drug Anal 23:294–302CrossRefGoogle Scholar
  18. Lu ZJ, Dockery CR, Crosby M, Chavarria K, Patterson B, Giedd M (2016) Antibacterial activities of wasabi against Escherichia coli O157:H7 and Staphylococcus aureus. Front Microbiol 7:1043Google Scholar
  19. Mahboubi A, Kamalinejad M, Ayatollahi AM, Babaeian M (2014) Total phenolic content and antibacterial activity of five plants of Labiatae against four foodborne and some other bacteria. Iran J Pharm Res 3:559–566Google Scholar
  20. Makkar HPS (1999) Quantification of tannins in tree foliage: a laboratory manual for the FAO/IAEA co-ordinated research project on Use nuclear and related techniques to develop simple tannin assay for predicting and improving the safety and efficiency of feeding ruminants on the tanniniferous tree foliage. In: Joint FAO/IAEA division of nuclear techniques in food and agriculture, ViennaGoogle Scholar
  21. Middleton E, Kandaswami C (1994) The impact of plant flavonoids on mammalian biology: implication for immunity, inflammation and cancer. In Harborne JB (ed) The flavonoids: advances in research since 1986, Chapman and Hall, London. pp. 619–952Google Scholar
  22. Miyake Y, Hiramitsu M (2011) Isolation and extraction of antimicrobial substances against oral bacteria from lemon peel. J Food Sci Technol 48:635–639CrossRefGoogle Scholar
  23. Mlambo NP (2008) The screening of medicinal plants traditionally used to treat diarrhoea, in Ongoye area, KwaZulu Natal, Thesis, pp. 50–52Google Scholar
  24. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  25. Nagarajan A, Arivalagan U, Rajagurua P (2011) In vitro root induction and studies on antibacterial activity of root extract of Costusigneus on clinically important human pathogens. J Microbiol Biotechnol Res 1:67–76Google Scholar
  26. Nisler J, Zatloukal M, Popa I, Doležal K, Strnad M, Spíchal M (2010) Cytokinin receptor antagonists derived from 6-benzylaminopurine. Phytochem 71:823–830CrossRefGoogle Scholar
  27. Romais E, Teixeira C, Ribeiro E, Lopes S (2000) Efeito do floroglucinol na reaçao morfogênica in vitro de segmentos internodais de Citrus sinensis (L.) Osbeck cv. Pera Rev Ceres 47:113–120Google Scholar
  28. Romeo L, Iori R, Rollin P, Bramanti P, Mazzon E (2018) Isothiocyanates: an overview of their antimicrobial activity against human infections. Molecules 23:624CrossRefGoogle Scholar
  29. SANBI (2015) Statistics: Red list of South African Plants version.2013.1. http://redlist.sanbi.org/stat.php
  30. Sarkar D, Naik PS (2000) Phloroglucinol enhances growth and rate of axillary shoot proliferation in potato shoot tip cultures in vitro. Plant Cell Tissue Org Cult 60:139–149CrossRefGoogle Scholar
  31. Shen B, Jensen RG, Bohnert HJ (1997) Mannitol protects against oxidation by hydroxyl radicals. Plant Physiol 115:527–532CrossRefGoogle Scholar
  32. Spíchal L, Werner T, Popa I, Riefler M, Schmulling T, Miroslav S (2009) The purine derivative PI55 blocks cytokinin action via receptor inhibition. FEBS J 276:244–253CrossRefGoogle Scholar
  33. Teixeira da Silva JA, Dobránszki J, Ross S (2013) Phloroglucinol in plant tissue culture. In Vitro Cell Dev Biol-Plant 49:1–16CrossRefGoogle Scholar
  34. Thiruvengadam M, Chung IM (2015) Phenolic compound production and biological activities from in vitro regenerated plants of gherkin (Cucumis anguria L.). Electron J Biotechnol 18:295–301CrossRefGoogle Scholar
  35. Vijaya SN, Udayasri PV, Aswani KY, Ravi BB, Phani KY, Vijay VM (2010) Advancements in the production of secondary metabolites. J Nat Prod 3:112–123Google Scholar
  36. Winkelmann K, San M, Kypriotakis Z, Skaltsa H, Bosilij B, Heilmann J (2003) Antibacterial and cytotoxic activity of prenylated bicyclic acylphloroglucinol derivatives from Hypericum amblycalyx. Z Naturforsch C J Biosci 58:527–532CrossRefGoogle Scholar
  37. Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Aloka Kumari
    • 1
  • Devashan Naidoo
    • 1
  • Ponnusamy Baskaran
    • 1
  • Karel Doležal
    • 2
  • Jaroslav Nisler
    • 2
  • Johannes Van Staden
    • 1
    Email author
  1. 1.Research Centre for Plant Growth and Development, School of Life SciencesUniversity of KwaZulu-Natal PietermaritzburgScottsvilleSouth Africa
  2. 2.Department of Chemical Biology and Genetics & Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University and Institute of Experimental BotanyAcademy of Sciences of Czech RepublicOlomouc-HoliceCzech Republic

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