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Plant Growth Regulation

, Volume 86, Issue 2, pp 323–331 | Cite as

Piriformospora indica improves salinity stress tolerance in Zea mays L. plants by regulating Na+ and K+ loading in root and allocating K+ in shoot

  • Ping Yun
  • Le Xu
  • Sai-Sai Wang
  • Lana Shabala
  • Sergey Shabala
  • Wen-Ying Zhang
Original paper
  • 173 Downloads

Abstract

Piriformospora indica is known as a fungus that can easily colonize a wide range of plants and enhance host’s growth and tolerance to abiotic stresses, including salinity. The mechanistic basis behind this phenomenon remains poorly understood. This work was aimed to fill in this gap and reveal mechanisms enhancing salinity tolerance in maize roots colonised by P. indica. A range of agronomic and physiological characteristics were compared between inoculated and non-inoculated maize plants under 0/100/200 mM NaCl conditions. The impact of P. indica inoculation or root’s cytosolic K+ retention ability were also assessed using micro-electrode ion flux estimation technique. The results showed that inoculated plants had higher biomass, higher stomatal conductance, lower K+ efflux from roots and higher potassium content in shoots than non-inoculated plants under salt stress. Collectively, the results indicated that the beneficial effects of inoculation on plant performance under saline conditions were mainly attributed to the improved stomata operation associated with higher rate of K delivery into the shoots.

Keywords

Piriformospora indica Salinity stress Potassium loading Micro-electrode ion flux estimation 

Abbreviations

ABA

Abscisic acid

AM

Arbuscular mycorrhizal

BSM

Basic salt medium

Ci

Intercellular CO2 concentration

DW

Dry weight

FW

Fresh weight

Gs

Stomatal conductance

MDA

Malondialdehyde

MIFE

Micro-electrode ion flux estimation

PGPR

Plant growth-promoting rhizobacteria

P. indica

Piriformospora indica

Pn

Net photosynthetic rate

TBA

Thiobarbituric acid

TCA

Trichloroacetic acid

Tr

Transpiration

Notes

Acknowledgements

The financial support was provided by Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education (KF201605), and the open fund of Hubei Collaborative Innovation Centre for Grain Industry (LXT-16-10).

Compliance with ethical standards

Conflict of interest

The authors have no conflicts of interest to this work.

Supplementary material

10725_2018_431_MOESM1_ESM.doc (1.4 mb)
Fig. S1 Experimental protocols and inoculation examination: (a) A schematic diagram of 23 the experimental protocols employed in this study. b, c - P. indica colonization of maize roots. (b) Control, (c) Root segments stained with trypan blue showing colonization of Z. mays by P.indica (arrow). Fig. S2 Photos of plants taken 5 days after onset of salt stress: (a) 0 mM NaCl +P. indica, (b) 100 mM NaCl +P. indica, (c) 200 mM NaCl +P. indica, (d) 0 mM NaCl -P. indica, (e) 100 mM NaCl -P. indica, (f) 200 mM NaCl -P. indica. Fig. S3 Photos of roots taken 5 days after onset of salt stress. (a) 0 mM NaCl +P. indica, (b) 100 mM NaCl +P. indica, (c) 200 mM NaCl +P. indica, (d) 0 mM NaCl -P. indica, (e) 100 mM NaCl -P. indica, (f) 200 mM NaCl -P. indica. (DOC 1451 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Hubei Collaborative Innovation Centre for Grain Industry/ School of AgricultureYangtze UniversityJingzhouChina
  2. 2.Tasmanian Institute of AgricultureUniversity of TasmaniaHobartAustralia
  3. 3.Department of HorticultureFoshan UniversityFoshanChina

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