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Plant Growth Regulation

, Volume 86, Issue 2, pp 287–296 | Cite as

Effects of co-inoculation of two different plant growth-promoting bacteria on duckweed

  • Yusuke Yamakawa
  • Rahul Jog
  • Masaaki Morikawa
Original paper
  • 107 Downloads

Abstract

Aseptic Lemna minor was soaked for 4 h in pond water where wild L. minor was naturally flourishing. Seven of the eight surface-colonizing bacterial strains were found capable of promoting the growth of L. minor. This high appearance of plant growth-promoting bacteria (PGPB) suggests that association of environmental bacteria is generally beneficial rather than harmful for host plants. One of the PGPB, Pseudomonas sp. Ps6, enhanced the growth of L. minor by 2–2.5-fold in 10 days. This activity was higher than that previously reported for Acinetobacter calcoaceticus P23, which enhanced growth of L. minor by 1.5–2-fold. Ps6 mostly adhered to and colonized the root rather than the frond, a leaf-like structure of duckweed where P23 preferentially adheres. It was expected that these two strains can share niches, coexist, and enhance the growth of duckweed additively upon co-inoculation. However, contrary to expectation, the growth of L. minor was enhanced by only 2.3-fold by co-inoculation of these two bacteria. P23 lowered the initial adhesion of Ps6 cells by 98.2% on the fronds and by 79.5% on the roots. However, initial adhesion of P23 cells to the roots increased dramatically, by 47.2-fold, following co-inoculation with Ps6. However, the number of P23 cells decreased dramatically to 0.7% on the root and to 3.6% on the frond after 10 days, whereas Ps6 cells increased by 12.5-fold on the frond and kept 69% on the root, thereby eventually restoring the population on the plant surfaces. Because duckweed is the fastest growing vascular plant and it is easy to grow an aseptic and axenic plant, the duckweed/bacteria co-culture system will be a model platform for studying multiple interactions among host plants and the associated bacteria.

Keywords

Lemna minor Plant growth-promoting bacteria Acinetobacter Pseudomonas Three-way symbiosis 

Abbreviation

PGPB

Plant growth-promoting bacteria

Notes

Acknowledgements

We are thankful to Dr. Kyoko Miwa (Hokkaido University) for her critical reading of our manuscript and a number of helpful suggestions. This study was supported by the Advanced Low Carbon Technology Research and Development Program (ALCA) of the Japan Science and Technology Agency (JST) Grant Number JPMJAL1108, Kobayashi International Scholarship Foundation, and Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 16K14844.

Author contributions

YY, RJ, and MM conceived and designed the research. YY performed most of the experiments with the help of RJ. YY and MM interpreted the data and wrote the manuscript. RJ revised the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Graduate School of Environmental ScienceHokkaido UniversitySapporoJapan

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