Plant Growth Regulation

, Volume 81, Issue 3, pp 533–542 | Cite as

β-Cyclodextrin–hemin enhances tolerance against salinity in tobacco seedlings by reestablishment of ion and redox homeostasis

  • Jing Zhang
  • Xincheng Yang
  • Yong Ren
  • Bo Yang
  • Ziwei Liu
  • Benwu You
  • Hongxiu Zhang
  • Wenbiao ShenEmail author
  • Xueping ChenEmail author
Original paper


β-Cyclodextrin–hemin (β-CDH) is a complex combining hemin with β-cyclodextrin (β-CD), which could improve hemin solubility. Our previous results showed that β-CDH, was able to enhance alfalfa tolerance against cadmium stress. However, whether or how β-CDH influences salinity tolerance is still elusive. In this report, we observed that similar to the beneficial responses of hemin rather than β-CD, the addition of β-CDH not only alleviated salinity-induced seedling growth inhibition (in particular), but also arrested chlorophyll degradation in tobacco seedlings. The efficiency of β-CDH against salinity stress compared to that of hemin, was confirmed, since the maximum beneficial responses against NaCl stress was obtained with 0.1 μM β-CDH and 10 μM hemin, respectively. Subsequent work showed that the redox imbalance caused by salinity stress could be improved by β-CDH. This was suggested by the reduced lipid peroxidation and hydrogen peroxide accumulation, as well as the induction of representative antioxidant genes, encoding superoxide dismutase, guaiacol peroxidase, and ascorbate peroxidase. Meanwhile, compared to control conditions, the ratio of K+ to Na+ was relatively low in NaCl-stressed tobacco seedlings. By contrast, the administration of β-CDH not only significantly blocked the increase of Na+, but also obviously increased K+, thus resulting in a high K+ to Na+ ratio in both shoot and root parts. Ion homeostasis is therefore reestablished. Together, our results suggested that β-CDH was able to improve salinity tolerance via the reestablishment of redox and ion homeostasis.


Nicotiana tabacum β-Cyclodextrin–hemin (β-CDH) Reactive oxygen species (ROS) Ion homeostasis Redox homeostasis 



This work was funded by the grant from China Tobacco Anhui Industrial, Co., Ltd (0920140421003), the National Natural Science Foundation of China (31170241), the Fundamental Research Funds for the Central Universities (KYTZ201402), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Author contributions

WS and XC designed the overall study and obtained funding; JZ, XY, YR, BY, ZL, BY, HZ carried out the experiments; JZ and WS analyzed and interpreted result; WS, JZ and XC wrote the first draft of the manuscript, and all authors approved the final version of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

10725_2016_230_MOESM1_ESM.doc (300 kb)
Supplementary material 1 (DOC 300 KB)


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Jing Zhang
    • 1
    • 2
  • Xincheng Yang
    • 3
  • Yong Ren
    • 4
  • Bo Yang
    • 5
  • Ziwei Liu
    • 2
  • Benwu You
    • 2
  • Hongxiu Zhang
    • 6
  • Wenbiao Shen
    • 1
    Email author
  • Xueping Chen
    • 2
    Email author
  1. 1.College of Life Sciences, Laboratory Center of Life SciencesNanjing Agricultural UniversityNanjingChina
  2. 2.Tobacco and Health Research CenterUniversity of Science and Technology of ChinaHefeiChina
  3. 3.College of HorticultureNanjing Agricultural UniversityNanjingChina
  4. 4.College of Life ScienceNanjing Normal UniversityNanjingChina
  5. 5.China Tobacco Anhui Industrial, Co., LtdHefeiChina
  6. 6.Nantong Feitian Chemical Industrial Co., LtdNantongChina

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