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Plant Growth Regulation

, Volume 79, Issue 1, pp 29–37 | Cite as

Effect of photosynthetic photon flux density on growth, photosynthetic competence and antioxidant enzymes activity during ex vitro acclimatization of Dieffenbachia cultivars

  • M. E. El-Mahrouk
  • Y. H. DewirEmail author
  • H. N. Murthy
  • H. Z. Rihan
  • H. S. Al-Shmgani
  • M. P. Fuller
Original paper

Abstract

The effects of 35, 70 and 100 µmol m−2 s−1 photosynthetic photon flux density (PPFD) were investigated on ex vitro acclimatization of micropropagated Dieffenbachia plants. Various growth characteristics, photosynthetic parameters and activities of antioxidant enzymes and dehydrins (DHN) were investigated. Fresh and dry plant biomass, plant height and root length were highest under the highest PPFD (100 µmol m−2 s−1), but this treatment was responsible for a reduction in the number of leaves. Chlorophyll and carotenoid contents and net photosynthesis were also optimal in plants grown under the highest irradiance. Stomatal resistance, transpiration rate and F v/F m values decreased with the incremental light irradiance. Activities of the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase were higher in the plants treated with 70 and 100 µmol m−2 s−1 PPFD. Accumulation of 55 kDa, 40 and 22 kDa DHN was observed in all light treatments. These results depict that lower PPFD (35 µmol m−2 s−1) was suitable for acclimatization of Dieffenbachia plants. High PPFD (>70 µmol m−2 s−1) induced accumulation of antioxidants and accumulation of DHN in the plants which reveals enhanced stress levels.

Keywords

Acclimatization Antioxidants Dieffenbachia Dehydrins Irradiance 

Abbreviations

CAT

Catalase

DHN

Dehydrins

Fv/Fm

Maximum quantum yield of photosystem II photochemistry

GPX

Glutathione peroxidase

PPFD

Photosynthetic photon flux density

ROS

Reactive oxygen species

SOD

Superoxide dismutase

RT

Room temperature

FW

Fresh weight

DW

Dry weight

Notes

Acknowledgments

This project was supported by King Saud University, Deanship of Scientific Research, College of Food & Agriculture Sciences, Agriculture Research Center. We would like to thank Prof. Timothy J. Close (Riverside University) for providing K-segment consensus peptide. We are also grateful to Angela Harrop (Plymouth University) for technical help.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • M. E. El-Mahrouk
    • 1
    • 2
  • Y. H. Dewir
    • 1
    • 3
    Email author
  • H. N. Murthy
    • 4
  • H. Z. Rihan
    • 2
    • 5
  • H. S. Al-Shmgani
    • 2
    • 6
  • M. P. Fuller
    • 2
  1. 1.Department of Horticulture, Faculty of AgricultureKafr El-Sheikh UniversityKafr El-SheikhEgypt
  2. 2.School of Biological Sciences, Faculty of Science and EnvironmentUniversity of PlymouthDrake CircusUK
  3. 3.Plant Production Department, College of Food and Agricultural ScienceKing Saud UniversityRiyadhSaudi Arabia
  4. 4.Department of BotanyKarnatak UniversityDharwadIndia
  5. 5.Horticulture Department, Faculty of AgricultureDamascus UniversityDamascusSyria
  6. 6.College of Education for Pure Science, Ibn Al-HaithamBaghdad UniversityBaghdadIraq

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