Plant Growth Regulation

, Volume 77, Issue 3, pp 299–306 | Cite as

Effects of sulfur supply and hydrogen peroxide pretreatment on the responses by rice under cadmium stress

  • Zeying Wu
  • Chunhua Zhang
  • Jiali Yan
  • Qian Yue
  • Ying Ge
Original paper
First Online:
Received:
Accepted:

Abstract

A hydroponic experiment was carried out to investigate the effects of sulfur (S) supply and hydrogen peroxide (H2O2) pretreatment on the growth and physiological responses by rice under cadmium (Cd) stress. Seedlings were grown with normal S level (3.4 mmol·L−1 sulfate), S deficiency (0 mmol·L−1 sulfate) for 6 days, or H2O2 (100 μmol·L−1) pretreatments for 1 day and subsequently exposed to 1.0 μmol·L−1 Cd for 7 days. In the S-deficient rice, both shoot and root Cd contents were increased 8.9 and 24.3 % respectively. In addition, cysteine (Cys), glutathione (GSH) and phytochelation (PC2–4) levels were increased significantly, while opposite effects were observed in the S-deficient rice. H2O2 pretreatment induced stronger Cd resistance in rice, as the amounts of thiols and the activity of glutathione S-transferase (GST) were all increased and the seedling growth was not inhibited by Cd stress. In conclusion, the results in this study demonstrated the adequate S supply and H2O2 pretreatment were important in Cd detoxification in rice through maintenance of cellular thiol levels and GST activity.

Keywords

Cadmium Sulfur supply Hydrogen peroxide Thiols Glutathione S-transferase 

Abbreviations

ACN

Acetonitrile

Cd

Cadmium

DTPA

Diethylenetriamine-pentaacetic acid

GSH

Reduced glutathione

GST

Glutathione S-transferase

HEPES

4-(2-Hydroxyethyl)-piperazine-1-propane sulfonic acid

mBBr

Monobromobimane

MSA

Methanesulfonic acid

PCS

Phytochelatin synthase

PCs

Phytochelatins

ROS

Reactive oxygen species

RP-UPLC

Reverse phase-ultra performance liquid chromatography

TBA

Thiobarbituric acid

TBARS

Thiobarbituric acid reactive substances

TFA

Trifluoroacetic acid

TCEP

Tris(β-chloroethyl)phosphate

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Notes

Acknowledgments

Financial support from the Natural Science Foundation of China (30700479) and Research Fund for the Doctoral Program of Higher Education of China (20090097110035, 20110097110004) and Research Fund of State Key Laboratory of Soil and Sustainable Agriculture, Nanjing Institute of Soil Science, Chinese Academy of Science (Y052010019) are gratefully acknowledged.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Zeying Wu
    • 1
  • Chunhua Zhang
    • 2
  • Jiali Yan
    • 1
  • Qian Yue
    • 1
  • Ying Ge
    • 1
  1. 1.Jiangsu Key Laboratory of Marine Biology, College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.Demonstration Laboratory of Elements and Life Science Research, Laboratory Centre of Life Science, College of Life ScienceNanjing Agricultural UniversityNanjingPeople’s Republic of China

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