Plant Growth Regulation

, Volume 74, Issue 3, pp 285–297 | Cite as

Kiwi fruit PMEI inhibits PME activity, modulates root elongation and induces pollen tube burst in Arabidopsis thaliana

  • Florence Paynel
  • Christelle Leroux
  • Ogier Surcouf
  • Annick Schaumann
  • Jérôme Pelloux
  • Azeddine Driouich
  • Jean Claude Mollet
  • Patrice Lerouge
  • Arnaud Lehner
  • Alain Mareck
Original paper

Abstract

Pectins are major components of primary cell wall that play a crucial role in plant development. After biosynthesis, pectins are secreted in the cell wall by Golgi-derived vesicles under a highly methylesterified form and are de-methylesterified by pectin methylesterases (PME). It is hypothesized that PME might be regulated by pectin methylesterase inhibitor (PMEI). In this paper, we show by isoelectric focalisation and subsequent zymogram that kiwi PMEI was able to inhibit Arabidopsis PME activity by forming a complex. The complexes were stable under a wide range of ionic strength and pH. Moreover, PMEI might be able to form a complex with basic PMEs including three PMEs strongly expressed in root and four PMEs expressed in pollen grains. Finally, exogenous treatment with kiwi PMEI was able to reduce the activity of cell wall resident PMEs with persistent effects such as an increase of the root growth and a dramatic effect on pollen tube stability.

Keywords

Pectin Pectin methylesterase Pectin methylesterase inhibitor Pollen Root growth Arabidopsis 

Abbreviations

HG

Homogalacturonan

IEF

Isoelectric focalisation

PME

Pectin methylesterase

PMEI

Pectin methylesterase inhibitor

RG-I

Rhamnogalacturonan-I

RG-II

Rhamnogalacturonan-II

Supplementary material

10725_2014_9919_MOESM1_ESM.tif (3.4 mb)
Fig S1 Electrophoresis gel of purified kiwi PMEI – (a) Molecular weight standards. (b) Purified kiwi PMEI (10 µg). (TIFF 3,477 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Florence Paynel
    • 1
  • Christelle Leroux
    • 1
  • Ogier Surcouf
    • 1
  • Annick Schaumann
    • 2
  • Jérôme Pelloux
    • 3
  • Azeddine Driouich
    • 1
  • Jean Claude Mollet
    • 1
  • Patrice Lerouge
    • 1
  • Arnaud Lehner
    • 1
  • Alain Mareck
    • 1
  1. 1.Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV) EA 4358Normandy University, University of Rouen, Institut de Recherche et d’Innovation BiomédicaleMont-Saint-AignanFrance
  2. 2.UMR-CNRS 6270Normandy University, University of Rouen, Institut de Recherche et d’Innovation BiomédicaleMont-Saint-AignanFrance
  3. 3.Laboratoire Biologie des Plantes et Innovation (BIOPI) EA3900University of Picardie Jules VerneAmiensFrance

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