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Plant Growth Regulation

, Volume 72, Issue 3, pp 249–255 | Cite as

Effect of sucrose concentration and seed maturity on in vitro germination of Dendrobium nobile hybrids

  • Waraporn Udomdee
  • Pei-Jung Wen
  • Chen-Yu Lee
  • Shih-Wen Chin
  • Fure-Chyi ChenEmail author
Original paper

Abstract

Orchid is a major floral crop around the world and Dendrobium hybrids are considered to be one of the most popular orchids. In vitro germination of hybrid seeds is a common practice among orchid growers, however, in many cross pollinations the embryos may not develop to maturity, leading to poor seed germination. The effect of seed maturity and sucrose concentration were investigated via asymbiotic germination of nobile Dendrobium hybrids. Capsules were harvested from two hybrids (Den. Lucky Girl × Den. Second Love ‘Kirameki’ and Den. Lucky Girl × Den. Hamana Lake ‘Kumi’) and one selfing of Den. Second Love ‘Kirameki’ at 2, 3, 4, and 5 months after pollination and immature seeds were taken. Immature seeds from 3- to 5-month old capsules could be successfully germinated on Hyponex based medium. Immature seeds from 4-month old capsules showed greatest germination rate of tested treatments, whereas 3-month old immature seeds showed the least germination. After 6 weeks of in vitro culture, protocorms derived from embryos developed on every concentration of sucrose, but germination was greater at lower concentrations. Greater concentration of sucrose decreased normal-developed protocorms.

Keywords

Nobile Dendrobium Asymbiotic seed germination Capsule maturity Embryo rescue 

Abbreviations

AC

Activated charcoal

CW

Coconut water

MS

Murashige and Skoog medium

PPF

Photosynthetic photon flux

Notes

Acknowledgments

The first author thanks National Pingtung University of Science and Technology for providing a scholarship during her PhD study. We also thank members of the laboratory of plant biotechnology, Department of plant industry, National Pingtung University of Science and Technology for assistance. This work was supported by grants supported by the Council of Agriculture, Taiwan with grant numbers 100AS-5.3.1-ST-aB and 101AS-5.3.1-ST-aB.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Waraporn Udomdee
    • 1
  • Pei-Jung Wen
    • 2
  • Chen-Yu Lee
    • 2
  • Shih-Wen Chin
    • 2
  • Fure-Chyi Chen
    • 2
    Email author
  1. 1.Department of Tropical Agriculture and International CooperationNational Pingtung University of Science and TechnologyPingtungTaiwan
  2. 2.Department of Plat IndustryNational Pingtung University of Science and TechnologyPingtungTaiwan

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