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Plant Growth Regulation

, Volume 71, Issue 3, pp 225–233 | Cite as

Polyphenol gene expression and changes in anthocyanins and polyphenols in the skin of ‘Braeburn’ apples after the autumn application of prohexadione-calcium

  • Jan BizjakEmail author
  • Nika Weber
  • Maja Mikulic-Petkovsek
  • Zobayer Alam
  • Jana Thill
  • Karl Stich
  • Heidi Halbwirth
  • Robert Veberic
Original paper

Abstract

Prohexadione-calcium (Pro-Ca) transient inhibits 2-Oxoglutarate-dependent dioxygenases and causes significant changes in the flavonoid spectrum of apple. In the present study the influence of two autumn preharvest applications of Pro-Ca on the polyphenol metabolism in apple peel during the advanced maturation was investigated. Pro-Ca was sprayed in two doses, approximately five and 3 weeks before the technological maturity. Changes in the concentrations of hydroxycinnamic acids, dihydrochalcones, flavonols, flavanols and anthocyanins as well as their related gene expression and enzyme activities in the apple peel were monitored six times during the advanced maturation until the technological maturity of the fruits. To evaluate its influence on red coloration differences in the chromatic values a*, h° and L* between the treated and untreated apples were monitored. The parameters showed a temporary effect of Pro-Ca on the intensity of red coloration, which was not detected anymore at the technological maturity of apples. The application of Pro-Ca decreased the flavanone 3-hydroxylase activity and slightly inhibited activities of all the enzymes analyzed. Concomitantly, the concentrations of anthocyanins in the peel of the treated apples decreased, whereas the concentrations of hydroxycinnamic acids, dihydrochalcones and flavan 3-ols increased. Flavonol concentrations, however, remained unchanged. The expression of ANS, ANR, FGT and MYB10 was downregulated after the Pro-Ca treatment. The results indicate that the autumn application of Pro-Ca modulates the biosynthetic pathway resulting in distinct changes in the flavonoid composition in the apple peel of ‘Braeburn’ apples. However, the changes are temporary and are generally suspended during apple storage.

Keywords

Flavonoids Anthocyanins Gene expression Phenolic acids Prohexadione-calcium 

Abbreviations

Pro-Ca

Prohexadione-calcium

BHT

2,6-di-tert-butyl-4-methylphenol

FW

Fresh weight

PAL

Phenylalanine ammonia-lyase

CHS/CHI

Chalcone synthase/chalcone isomerase

FHT

Flavanone 3-hydroxylase

DFR

Dihydroflavonol 4-reductase

ANS

Anthocyanidin synthase

ANR

Anthocyanidin reductase

FGT

Flavonoid 3-O-glycosyltransferase

EF

Elongation factor

Notes

Acknowledgments

This investigation was part of the Horticulture Program P4-0013-0481 and the authors would like to thank the Slovenian Ministry of Higher Education, Science and Technology for the financial support. The work was performed within the frame of PlantEngine (Cost Action FA1006).

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Jan Bizjak
    • 1
    Email author
  • Nika Weber
    • 1
  • Maja Mikulic-Petkovsek
    • 1
  • Zobayer Alam
    • 2
  • Jana Thill
    • 2
  • Karl Stich
    • 2
  • Heidi Halbwirth
    • 2
  • Robert Veberic
    • 1
  1. 1.Department of Agronomy, Biotechnical Faculty, Chair for Fruit, Wine and Vegetable GrowingUniversity of LjubljanaLjubljanaSlovenia
  2. 2.Institute for Chemical EngineeringTechnical University of ViennaViennaAustria

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