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Plant Growth Regulation

, Volume 70, Issue 3, pp 297–303 | Cite as

RETRACTED ARTICLE: Synthetic seed technology for short term conservation of medicinal orchid Dendrobium densiflorum Lindl. Ex Wall and assessment of genetic fidelity of regenerants

  • P. Mohanty
  • J. DasEmail author
Brief communication

Abstract

Artificial seeds were obtained through encapsulation of protocorm-like bodies (PLBs) of Dendrobium densiflorum in calcium alginate beads. This paper demonstrates the alginate-encapsulation and conversion (complete plantlet regeneration) from PLBs, the effect of storage conditions (at different temperature; 4, 8, 16 °C, RT and duration; 15, 30, 45, 60, 75, 90 days) on viability of encapsulated plant materials as well as the assessment of genetic fidelity of the regenerants. Individual PLBs were encapsulated in calcium alginate beads for mass propagation, short-term storage and germplasm sharing. The superior gel matrix for encapsulation was obtained using 3 % sodium alginate and 100 mM calcium chloride (CaCl2·2H2O). The highest percentage of conversion (100 %) of encapsulated PLBs (capsules) was obtained on MS2 medium (MS medium + 2 mg/l BAP). Capsules were successfully stored till 60 days at 8 °C with conversion frequency of 95.5 %. Plantlets regenerated from encapsulated beads were acclimatized successfully with 95 % survival rate. A total of 40 primers were screened, out of which 10 primers successfully generated 39 scorable bands, ranging from 0.2 to 1.3 kb amplicons. The uniform RAPD banding profile among the plantlets derived from encapsulated PLBs following 60 days of storage confirmed genetic fidelity.

Keywords

Short term storage Dendrobium densiflorum Encapsulation Genetic fidelity 

Abbreviations

PLB

Protocorm like bodies

MS

Murashige and Skoog medium

BAP

6-Benzyl aminopurine

IBA

Indole-3 butyric acid

RAPD

Random amplified polymorphic DNA

Notes

Acknowledgments

Authors are thankful to DBT, Government of India for providing the financial assistance to carry out the research work.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.National Research Centre for OrchidsPakyongIndia
  2. 2.Plant Bioresources Division, Institute of Bioresources and Sustainable Development (IBSD)Sikkim CentreTadongIndia

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