Accumulation of red elemental selenium nanoparticles and their biological effects in Nicotinia tabacum
- 705 Downloads
The uptake, accumulation and biological effects of red nano-sized elemental selenium (nanoSe) in comparison to selenate were investigated in plant system at the first time. The data clearly indicated that red nanoSe was taken up by tobacco callus cultures and rooted tobacco plantlets. The roots of regenerated plantlets accumulated selenium in very high concentrations, 2,947 ± 99 mg/kg DW, from the medium containing 530 μM nanoSe. The biological effects of nanoSe were different from the selenate ion in plant tissue culture. NanoSe (265–530 μM concentration range) stimulated the organogenesis and the growth of root system significantly (~40 %) while selenate did not show these effects at any concentration moreover inhibited both callus growth and root regeneration totally in 265–530 μM concentrations.
KeywordsElemental nanoselenium particles Organogenesis Selenate ion Morphogenesis
This work was supported partly by National Research and Technological Office (NKTH, Budapest, Hungary, Project no. EA-2044-010/2009, Ereky Foundation, Debrecen, Hungary) and partly by the MOP Biotech Ltf, and Pro-Team Ltf Nyíregyháza, Hungary. We are grateful to Dr. Mihály Czakó (University of South Carolina, Department of Biological Sciences, Columbia) for revising the manuscript.
- Eszenyi P, Sztrik A, Babka B, Prokisch J (2011) Elemental, nano-sized (100–500 nm) selenium production by probiotic lactic acid bacteria. Inter J Biosci Biochem Bioinform 1:148–152Google Scholar
- Hurd-Karrer AM (1935) Factors affecting the absorption of selenium from soils by plants. J Agric Res 50:413–427Google Scholar
- Prokisch J, Zommara M (2008) Process for producing elemental selenium nanospheres PCT/IB2008/052838 date of receipt: 15 July 2008 receiving office: International Bureau of the World intellectual property organization your reference: P104315Google Scholar
- Prokisch J, Széles É, Kovács B, Daróczy L, Zommara M (2008) Formation of metal selenium nanospheres in bacteria: is it a possible detoxification mechanism? Cer Res Comm 36:947–995Google Scholar
- White PJ, Bowen HC, Parmaguru P, Fritz M, Spracklen WP, Spiby RE, Meacham MC, Mead A, Harriman M, Trueman LJ, Smith BM, Thomas B, Broadley MR (2004) Interactions between selenium and sulphur nutrition in Arabidopsis thaliana. J Exp. Bot 55:1927–1937. doi: 10.1093/jxb/erh192 PubMedCrossRefGoogle Scholar