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Plant Growth Regulation

, Volume 68, Issue 3, pp 459–465 | Cite as

Efficiency of Agrobacterium rhizogenes–mediated root transformation of Parasponia and Trema is temperature dependent

  • Qingqin Cao
  • Rik Op den Camp
  • Maryam Seifi Kalhor
  • Ton Bisseling
  • Rene Geurts
Original paper

Abstract

Parasponia trees are the only non-legume species that form nitrogen-fixing root nodules with rhizobium. Based on its taxonomic position in relation to legumes (Fabaceae), it is most likely that both lineages have gained this symbiotic capacity independently. Therefore, Parasponia forms a bridging species to understand the evolutionary constraints underlying this symbiosis. However, absence of key technologies to genetically modify Parasponia seriously impeded studies on these species. We employed Agrobacterium rhizogenes to create composite Parasponia andersonii plants that harbour transgenic roots. Here, we provide an optimized protocol to infect P. andersonii as well as its non-symbiotic sister species Trema tomentosa with A. rhizogenes. We show that the transformation efficiency is temperature dependent. Whereas the optimal growth temperature for both these species is 28 °C, the transformation is most efficient when co-cultivation with A. rhizogenes occurs at 21 °C. Using this optimized protocol up to 80 % transformation efficiency can be obtained. These robust transformation platforms will provide a strong tool to unravel the Parasponia–rhizobium symbiosis.

Keywords

Parasponia Trema Agrobacterium transformation Symbiosis Transgenic root Composite plant 

Notes

Acknowledgments

This work was supported by the Dutch Science Foundation (NWO) (VIDI 864.06.007 to R.G.) and a visitors fellowship of the Dutch Graduate School of Experimental Plant Sciences to Q.C.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Qingqin Cao
    • 1
    • 2
  • Rik Op den Camp
    • 2
  • Maryam Seifi Kalhor
    • 2
  • Ton Bisseling
    • 2
    • 3
  • Rene Geurts
    • 2
  1. 1.Department of BiotechnologyBeijing University of AgricultureBeijingPeople’s Republic of China
  2. 2.Laboratory of Molecular Biology, Department of Plant SciencesWageningen UniversityWageningenThe Netherlands
  3. 3.King Saud University, College of ScienceRiyadhSaudi Arabia

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