Plant Growth Regulation

, Volume 68, Issue 2, pp 249–259 | Cite as

24-Epibrassinolide alleviated zinc-induced oxidative stress in radish (Raphanus sativus L.) seedlings by enhancing antioxidative system

  • Bellamkonda Ramakrishna
  • S. Seeta Ram Rao
Original paper


This article encompasses the results on the effects of 24-epibrassinolide (EBR) on the changes in reactive oxygen species (ROS) and activities of antioxidative enzymes in radish (Raphanus sativus L.) seedlings subjected to zinc (Zn) stress. Zn toxicity resulted in significant enhancement in the level of membrane lipid peroxidation, protein oxidation, contents of hydrogen peroxide (H2O2) and hydroxyl radical (·OH), the production rate of superoxide radicals (O 2 ·− ) and the activities of lipoxygenase and NADPH oxidase in radish seedlings indicating the induction of oxidative stress. However, Zn-mediated enhancement in indices of oxidative stress was considerably decreased by EBR treatment. EBR application enhanced the activities of catalase, superoxide dismutase, guaiacol peroxidase, glutathione peroxidase, and peroxidase in radish seedlings under Zn stress. EBR treatment reduced the activity of ascorbic acid oxidase in Zn stressed seedlings. Further, EBR application also enhanced the free proline and phenol levels under Zn stress. From the results obtained in this study, it can be inferred that EBR application alleviated oxidative damage caused by over production of ROS through the up regulation of antioxidative capacity in Zn stressed radish seedlings.


Antixoidative system 24-Epibrassinolide Lipid peroxidation Protein carbonyls ROS Zinc stress 



The financial support to BRK under the UGC-RFSMS Scheme from University Grants Commission, New Delhi, India is greatly acknowledged.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Department of BotanyOsmania UniversityHyderabadIndia

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