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Plant Growth Regulation

, Volume 68, Issue 2, pp 249–259 | Cite as

24-Epibrassinolide alleviated zinc-induced oxidative stress in radish (Raphanus sativus L.) seedlings by enhancing antioxidative system

  • Bellamkonda Ramakrishna
  • S. Seeta Ram Rao
Original paper

Abstract

This article encompasses the results on the effects of 24-epibrassinolide (EBR) on the changes in reactive oxygen species (ROS) and activities of antioxidative enzymes in radish (Raphanus sativus L.) seedlings subjected to zinc (Zn) stress. Zn toxicity resulted in significant enhancement in the level of membrane lipid peroxidation, protein oxidation, contents of hydrogen peroxide (H2O2) and hydroxyl radical (·OH), the production rate of superoxide radicals (O 2 ·− ) and the activities of lipoxygenase and NADPH oxidase in radish seedlings indicating the induction of oxidative stress. However, Zn-mediated enhancement in indices of oxidative stress was considerably decreased by EBR treatment. EBR application enhanced the activities of catalase, superoxide dismutase, guaiacol peroxidase, glutathione peroxidase, and peroxidase in radish seedlings under Zn stress. EBR treatment reduced the activity of ascorbic acid oxidase in Zn stressed seedlings. Further, EBR application also enhanced the free proline and phenol levels under Zn stress. From the results obtained in this study, it can be inferred that EBR application alleviated oxidative damage caused by over production of ROS through the up regulation of antioxidative capacity in Zn stressed radish seedlings.

Keywords

Antixoidative system 24-Epibrassinolide Lipid peroxidation Protein carbonyls ROS Zinc stress 

Notes

Acknowledgments

The financial support to BRK under the UGC-RFSMS Scheme from University Grants Commission, New Delhi, India is greatly acknowledged.

References

  1. Aebi H (1974) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Verlag Chemie/Academic Press Inc., Weinheim/NewYork, pp 673–680Google Scholar
  2. Ali B, Hayat S, Fariduddin Q, Ahmad A (2008) 24-Epibrassinolide protects against the stress generated by salinity and nickel in Brassica juncea. Chemosphere 72:1387–1392PubMedCrossRefGoogle Scholar
  3. Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53:1331–1341PubMedCrossRefGoogle Scholar
  4. Andre CM, Yvan L, Daniele E (2010) Dietary antioxidants and oxidative stress from a human and plant perspective: a review. Curr Nutr Food Sci 6:2–12CrossRefGoogle Scholar
  5. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399PubMedCrossRefGoogle Scholar
  6. Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216CrossRefGoogle Scholar
  7. Bajguz A (2000) Effect of brassinosteroids on nucleic acids and protein content in cultured cells of Chlorella vulgaris. Plant Physiol Biochem 38:209–215CrossRefGoogle Scholar
  8. Bajguz A, Hayat S (2009) Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiol Biochem 47:1–8PubMedCrossRefGoogle Scholar
  9. Bari R, Jones JDG (2009) Role of plant hormones in plant defense responses. Plant Mol Biol 69:473–488PubMedCrossRefGoogle Scholar
  10. Bates L, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  11. Beauchamp CO, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287PubMedCrossRefGoogle Scholar
  12. Behnamnia M, Kalantari KM, Rezanejad F (2009) Exogenous application of brassinosteroid alleviates drought-induced oxidative stress in Lycopersicon esculentum L. Gen Appl Plant Physiol 35:22–34Google Scholar
  13. Çağ S, Gören-Sağlam N, Çıngıl-Barış C, Kaplan E (2007) The effect of different concentration of epibrassinolide on chlorophyll, protein and anthocyanin content and peroxidase activity in excised red cabbage (Brassica oleracea L.) cotyledons. Biotechnol Biotechnol Equip 21:422–425Google Scholar
  14. Cao S, Xu Q, Cao Y, Qian K, An K, Zhu Y, Binzeng H, Zhao H, Kuai B (2005) Loss of function mutations in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis. Physiol Plant 123:57–66CrossRefGoogle Scholar
  15. Castiglione S, Franchin C, Fossat T, Lingua G, Torrigiani P, Biondi S (2007) High zinc concentrations reduce rooting capacity and alter metallothionein gene expression in white poplar (Populus alba L. cv. Villafranca). Chemosphere 67:1117–1126PubMedCrossRefGoogle Scholar
  16. Choudhary SP, Bhardwaj R, Gupta BD, Dutt P, Gupta RK, Kanwar M, Biondi S (2011) Enhancing effects of 24-epibrassinolide and putrescine on the antioxidant capacity and free radical scavenging activity of Raphanus sativus seedlings under Cu ion stress. Acta Physiol Plant 33:1319–1333CrossRefGoogle Scholar
  17. Contreras L, Mella D, Moenne A, Correa JA (2009) Differential responses to copper-induced oxidative stress in the marine macroalgae Lessonia nigrescens and Scytosiphon lomentaria (Phaeophyceae). Aquat Toxicol 94:94–102PubMedCrossRefGoogle Scholar
  18. De Magalhaes CCP, Cardoso D, Dos Santos CP, Chaloub RM (2004) Physiological and photosynthetic responses of Synechocystis aquatilis f. aquatilis (Cyanophyceae) to elevated levels of zinc. J Phycol 40:496–504CrossRefGoogle Scholar
  19. Divi UK, Krishna P (2009) Brassinosteroid: a biotechnological target for enhancing crop yield and stress tolerance. New Biotechnol 26:131–136CrossRefGoogle Scholar
  20. Ederli M, Leuschen MP, Eirefaey H, Hamada FM, Rojas P (1996) The antioxidant properties of zinc and metallothionein. Neurochem Int 29:159–166CrossRefGoogle Scholar
  21. Esaka M, Imagi J, Suzuki K, Kubota K (1988) Formation of ascorbate oxidase in cultured cells. Plant Cell Physiol 39:231–235Google Scholar
  22. Faroo M, Wahid A, Basra SM, Islam-ud-Din (2009) Improving water relations and gas exchange with brassinostroids in rice under drought stress. J Agron Crop Sci 195:262–269CrossRefGoogle Scholar
  23. Foyer CH, Looez-Delgado H, Dat JF, Scott IM (1997) Hydrogen peroxide and glutathione associated mechanisms of acclamatory stress tolerance and signaling. Physiol Plant 100:241–254CrossRefGoogle Scholar
  24. Gayor A, Srivastava PS, Iqbal M (1999) Morphogenic and biochemical responses of Bacopa monniera cultures to zinc toxicity. Plant Sci 143:187–193CrossRefGoogle Scholar
  25. Gonçalves JF, Becker AG, Cargnelutti D, Tabaldi LA, Pereira LB, Battisti V, Spanevello RM, Morsch VM, Nicoloso FT, Schetinger MRC (2007) Cadmium toxicity causes oxidative stress and induces response of the antioxidant system in cucumber seedlings. Braz J Plant Physiol 19:223–232Google Scholar
  26. Haliwell B, Grootveld M, Gutteridge JMC (1988) Methods for the measurement of hydroxyl radicals in biochemical system: dioxyribose degradation and aromatic hydroxylation. Methods Biochem Anal 33:59–90CrossRefGoogle Scholar
  27. Hao F, Wang X, Chen J (2006) Involvement of plasma-membrane NADPH oxidase in nickel induced oxidative stress in roots of wheat seedlings. Plant Sci 170:151–158CrossRefGoogle Scholar
  28. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts 1. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 12:189–198CrossRefGoogle Scholar
  29. Jiang M, Zhang J (2002) Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings. Planta 215:1022–1030PubMedCrossRefGoogle Scholar
  30. Kar M, Mishra D (1976) Catalase, peroxidase and polyphenol oxidase activities during rice leaf senescence. Plant Physiol 57:315–319PubMedCrossRefGoogle Scholar
  31. Kulaeva ON, Burkhanova EA, Fedina AB et al (1991) Effect of brassinosteroids on protein synthesis and plant-cell ultra structure under stress conditions. In: Culter HG, Yokota T, Adam G (eds) Brassinosteroids—chemistry, bioactivity and applications. American Chemical Society Symposium Series, Washington, pp 141–157CrossRefGoogle Scholar
  32. Liu YJ, Jiang HJ, Zhao ZG, An LZ (2011) Abscisic acid is involved in brassinosteroids-induced chilling tolerance in the suspension cultured cells from Chorispora bungeana. J Plant Physiol 168:853–862PubMedCrossRefGoogle Scholar
  33. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  34. Lutts S, Kinet JM, Bouharmont J (1996) NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Anal Bot 78:389–398CrossRefGoogle Scholar
  35. Mazhoudi S, Chaoui A, Ghorbal MH, El Ferjani E (1997) Response of antioxidant enzymes to excess copper in tomato (Lycopersicon esculentum, Mill.). Plant Sci 127:129–137CrossRefGoogle Scholar
  36. Michalak A (2006) Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish J Environ Stud 15:523–530Google Scholar
  37. Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410PubMedCrossRefGoogle Scholar
  38. Morina F, Jovanovicb L, Mojovicc M, Vidovica M, Pankovicd D, Jovanovic SV (2010) Zinc-induced oxidative stress in Verbascum thapsus is caused by an accumulation of reactive oxygen species and quinhydrone in the cell wall. Physiol Plant 140:209–224PubMedGoogle Scholar
  39. Mukherjee SP, Choudhari MA (1983) Implications for water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:166–170CrossRefGoogle Scholar
  40. Nagalakshmi N, Prasad MNV (2001) Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus. Plant Sci 160:291–299PubMedCrossRefGoogle Scholar
  41. Rao KVM, Sresty TS (2000) Antioxidative parameters in the seedlings of pigeon pea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses. Plant Sci 157:113–128CrossRefGoogle Scholar
  42. Rao SSR, Vardhini BV, Sujatha E, Anuradha S (2002) Brassinosteroids: a new class of phytohormones. Curr Sci 82:1239–1245Google Scholar
  43. Reznick AZ, Packer L (1994) Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 233:357–363PubMedCrossRefGoogle Scholar
  44. Rout GR, Das P (2003) Effect of metal toxicity on plant growth and metabolism: I. Zinc. Agronomie 23:3–11CrossRefGoogle Scholar
  45. Sarkar RK, Das A (2000) Changes in antioxidative enzymes and antioxidants in relation to flooding tolerance in rice. J Plant Biol 27:307–311Google Scholar
  46. Sasse JM (2003) Physiological actions of brassinosteroids: an update. J Plant Growth Regul 22:276–288PubMedCrossRefGoogle Scholar
  47. Swain T, Hillis WE (1959) The phenolic constituents of Prunus domestica L. the quantitative analysis of phenolic constituents. J Sci Food Agri 10:63–68CrossRefGoogle Scholar
  48. Tewari RK, Kumar P, Sharma PN (2008) Morphology and physiology of zinc-stressed mulberry plants. J Plant Nutr Soil Sci 171:286–294CrossRefGoogle Scholar
  49. Vaillant N, Monnet F, Hitmi A, Sallanon H, Coudret A (2005) Comparative study of responses in four Datura species to zinc stress. Chemosphere 59:1005–1013PubMedCrossRefGoogle Scholar
  50. Von Tiedemann A (1997) Evidence for a primary role of active oxygen species in induction of host cell death during infection of bean leaves with Botrytis cinerea. Physiol Mol Plant Pathol 50:151–166CrossRefGoogle Scholar
  51. Wang C, Zhang SH, Wang PF, Qian J, Hou J, Zhang WJ, Lu J (2009a) Excess Zn alters the nutrient uptake and induces the antioxidative responses in submerged plant Hydrilla verticillata (L.f.) Royle. Chemosphere 76:938–945PubMedCrossRefGoogle Scholar
  52. Wang H, Feng T, Peng X, Yan M, Zhou P, Tang X (2009b) Ameliorative effects of bassinosteroid on excess manganese-induced oxidative stress in Zea mays L. leaves. Agric Sci China 8:1063–1074CrossRefGoogle Scholar
  53. Xia XJ, Wang YJ, Zhou YH, Tao Y, Mao WH, Shi K, Asami T, Chen ZX, Yu JQ (2009) Reactive oxygen species are involved in brassinosteroid—induced stress tolerance in cucumber. Plant Physiol 150:801–814PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Department of BotanyOsmania UniversityHyderabadIndia

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