Plant Growth Regulation

, Volume 68, Issue 2, pp 313–317 | Cite as

Comparative mineral and hormonal analyses of wild type and TLS somaclonal variant derived from oil palm (Elaeis guineensis Jacq. var. tenera) tissue culture

  • S. H. Habib
  • S.-E. Ooi
  • Ondřej Novák
  • Danuše Tarkowská
  • Jakub Rolčík
  • Karel Doležal
  • S. S. R. Syed-Alwee
  • C.-L. Ho
  • P. Namasivayam
Brief communication

Abstract

Comparative mineral and hormonal analyses were made on tissue culture derived truncated leaf syndrome and wild type oil palm seedlings. Mineral analysis confirmed that Boron, Zinc and chlorophyll levels were significantly lower in truncated leaf syndrome leaves than those of wild type. Hormonal analysis also revealed various cytokinin derivatives such as trans-zeatin, trans-zeatin riboside, trans-zeatin O-glucoside and trans-zeatin riboside 5′mono phosphate were significantly higher in truncated leaf syndrome leaves compared to wild type leaves. Brassinolide level was also significantly higher in truncated leaf syndrome leaves than those of the wild type. These observations suggest that the truncated leaf syndrome abnormality could be associated to high cytokinin and brassinosteroid production which affects the uptake of Boron and Zinc.

Keywords

Truncated leaf syndrome Boron Zinc Cytokinin Brassinosteroid Brassinolide Oil palm Abnormality 

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • S. H. Habib
    • 1
  • S.-E. Ooi
    • 3
  • Ondřej Novák
    • 4
  • Danuše Tarkowská
    • 4
  • Jakub Rolčík
    • 4
  • Karel Doležal
    • 4
    • 6
  • S. S. R. Syed-Alwee
    • 2
  • C.-L. Ho
    • 1
    • 5
  • P. Namasivayam
    • 1
    • 5
  1. 1.Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Felda Biotechnology Centre, PT 23417, Lengkuk Technology, Persiaran TechnologyTechpark @ EnstekBandar EnstekMalaysia
  3. 3.Malaysian Palm Oil BoardKajangMalaysia
  4. 4.Laboratory of Growth Regulators, Faculty of SciencePalacký University and Institute of Experimental Botany ASCROlomoucThe Czech Republic
  5. 5.Institute of Tropical AgricultureUniversiti Putra MalaysiaSerdangMalaysia
  6. 6.Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of SciencePalacký UniversityOlomoucThe Czech Republic

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