Comparative mineral and hormonal analyses of wild type and TLS somaclonal variant derived from oil palm (Elaeis guineensis Jacq. var. tenera) tissue culture
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Comparative mineral and hormonal analyses were made on tissue culture derived truncated leaf syndrome and wild type oil palm seedlings. Mineral analysis confirmed that Boron, Zinc and chlorophyll levels were significantly lower in truncated leaf syndrome leaves than those of wild type. Hormonal analysis also revealed various cytokinin derivatives such as trans-zeatin, trans-zeatin riboside, trans-zeatin O-glucoside and trans-zeatin riboside 5′mono phosphate were significantly higher in truncated leaf syndrome leaves compared to wild type leaves. Brassinolide level was also significantly higher in truncated leaf syndrome leaves than those of the wild type. These observations suggest that the truncated leaf syndrome abnormality could be associated to high cytokinin and brassinosteroid production which affects the uptake of Boron and Zinc.
KeywordsTruncated leaf syndrome Boron Zinc Cytokinin Brassinosteroid Brassinolide Oil palm Abnormality
The authors thank TWOWS (Third World Organization for Women in Sciences) for the postgraduate research fellowship to Ms. Habib S.H., the Ministry of Higher Education, Malaysia for funding this project through the FRGS grant (05-10-07-364FR), FELDA Agricultural Services Sdn. Bhd. for providing the oil palm seedlings and Czech Ministry of Education, grant No. ED0007/01/01 Centre of the Region Haná for Biotechnological and Agricultural Research for financial support.
- Abdullah R, Zainal A, Heng WY, Li C, Beng YC, Phing LM, Sirajuddin SA, Ping WYS, Joseph JL (2005) Immature embryo: a useful tool for oil palm (Elaeis guineesnsis Jacq.) genetic transformation studies [Electronic version]. J Biotechnol 8:25–34Google Scholar
- Gupta U (1993) Boron and its role in crop production. CRC Press, FloridaGoogle Scholar
- Jones JB (1991) Plant analysis handbook. Micro-Macro Publishers, GeorgiaGoogle Scholar
- Oliveira RH, Rosolem CA, Moraes-Dallaqua MH (2003) Alterações na anatomia do algodoeiro induzidas pela deficiência de boro. In: Congresso Brasileiro De Ciência Do Solo, 24., Ribeirão Preto, 2003. Anais. Ribeirão Preto, SBCS/UNESPGoogle Scholar
- Pilipenko TI, Solv’eva NS (1979) Accumulation of zinc in tissue of Phascolus vulguris plants supplied with different boron rates. Referativnyi Zhurnal Biologya 189:71–74. In: Shorrocks VM (ed) Zinc in agriculture 3(3):1983. A quarterly bulletin. Micronutrient bureau, M.B. House, Wigginton, Tring, Hertfordshire HP23 6ED, UKGoogle Scholar
- Ramage CM, Williams RR (2004) Cytokinin-induced abnormal shoot organogenesis is associated with elevated Knotted1-type homeobox gene expression in tobacco. Plant Cell Rep 22:919–924Google Scholar
- Tabori-Magyar K, Dobranszki J, Teixeira da Silva JA, Bulley SM, Hudak I (2010) The role of cytokinins in shoot organogenesis in apple. Plant Cell Tiss Org Cult. doi: 10.1007/s11240-010-9696-6
- Tan YP, Ho YW, Sharma M (1996) Truncated leaf symptoms (TLS) on clonal seedlings. Int Soc Oil Palm Breeders Newslett 12:1–5Google Scholar
- Tan CC, Wong G, Sohl AC (1999) Acclimatization and handling of oil palm tissue cultured plantlets for large scale commercial production. Paper presented at PORIM Intl. Palm Oil Congress 1–6 Feb, KLGoogle Scholar