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Plant Growth Regulation

, Volume 67, Issue 1, pp 101–105 | Cite as

Gradually scale-up culture in a bioreactor promotes radical scavenging activity of Panax ginseng (C. A. Meyer) adventitious roots on 1,1-diphenyl-2-picrylhydrazyl

  • Juan Wang
  • Wenyuan GaoEmail author
  • Beimei Zuo
  • Hui Liu
  • Liming Zhang
  • Luqi Huang
Brief communication

Abstract

A scale-up culture of adventitious roots of ginseng was established using a balloon-type bubble bioreactor (BTBB). Maximum growth rates of ~52-fold and ~50-fold in 3 and 5 L BTBBs were obtained, respectively after 40 days of inoculation, which was significantly higher than that in 0.5 L conical flask (~15-fold). Gradually scale-up culture of adventitious roots increased the root biomass, while the contents of ginsenoside and polysaccharides were not affected. This study also revealed that radical scavenging activity of dried adventitious roots on 1,1-diphenyl-2-picrylhydrazyl was higher than that of native roots at 20–100 mg L−1 methanolic extract.

Keywords

Adventitious root Antioxidant Bioreactor culture Ginsenoside Panax ginseng Polysaccharide 

Abbreviations

2, 4-D

2, 4-Dichlorophenoxyacetic acid

IBA

Indole-3-butric acid

MS

Murashige and Skoog (1962)

BTBB

Balloon-type bubble bioreactor

Vvm

Volumes of gas per a bioreactor volume

DPPH

1,1-Diphenyl-2-picrylhydrazyl

Notes

Acknowledgments

This research was funded by Science and Technology Commission of Tianjin, China (09ZCKFSH01100).

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Juan Wang
    • 1
    • 2
  • Wenyuan Gao
    • 1
    • 2
    Email author
  • Beimei Zuo
    • 2
  • Hui Liu
    • 2
  • Liming Zhang
    • 2
  • Luqi Huang
    • 3
  1. 1.School of Pharmaceutical Science and TechnologyTianjin UniversityTianjinChina
  2. 2.Institute of Biological Engineering of Traditional Chinese MedicineTianjin University of Science and TechnologyTianjinChina
  3. 3.Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijingChina

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