Gradually scale-up culture in a bioreactor promotes radical scavenging activity of Panax ginseng (C. A. Meyer) adventitious roots on 1,1-diphenyl-2-picrylhydrazyl
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A scale-up culture of adventitious roots of ginseng was established using a balloon-type bubble bioreactor (BTBB). Maximum growth rates of ~52-fold and ~50-fold in 3 and 5 L BTBBs were obtained, respectively after 40 days of inoculation, which was significantly higher than that in 0.5 L conical flask (~15-fold). Gradually scale-up culture of adventitious roots increased the root biomass, while the contents of ginsenoside and polysaccharides were not affected. This study also revealed that radical scavenging activity of dried adventitious roots on 1,1-diphenyl-2-picrylhydrazyl was higher than that of native roots at 20–100 mg L−1 methanolic extract.
KeywordsAdventitious root Antioxidant Bioreactor culture Ginsenoside Panax ginseng Polysaccharide
- 2, 4-D
2, 4-Dichlorophenoxyacetic acid
Murashige and Skoog (1962)
Balloon-type bubble bioreactor
Volumes of gas per a bioreactor volume
This research was funded by Science and Technology Commission of Tianjin, China (09ZCKFSH01100).
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