Plant Growth Regulation

, Volume 65, Issue 3, pp 449–457 | Cite as

Enhanced biomass and steviol glycosides in Stevia rebaudiana treated with phosphate-solubilizing bacteria and rock phosphate

  • Mamta Gupta
  • Shashi Bisht
  • Bikram Singh
  • Arvind Gulati
  • Rupinder Tewari
Original paper


Biofertilizers offer alternative means to promoting cultivation of medicinal plants less dependent on chemical fertilizers. Present study was aimed at evaluating the potential of phosphate-solubilizing bacteria (PSB) Burkholderia gladioli MTCC 10216, B. gladioli MTCC 10217, Enterobacter aerogenes MTCC 10208 and Serratia marcescens MTCC 10238 for utilizing Mussoorie rock phosphate (MRP) to enhance plant growth, and stevioside (ST) and rebaudioside-A (R-A) contents of Stevia rebaudiana. The solubilization of MRP by PSB strains varied from 1.4 to 15.2 μg ml−1, with the highest solubilization by Enterobacter aerogenes 10208. The PSB treatment increased the growth and ST and R-A contents of plants. Plant growth and stevioside contents were more pronounced with plants treated with a mixture of strains and grown in MRP amended soil compared to the unamended soil. The increment in shoot length (47.8%), root length (17.4%), leaf dry weight (164%), stem dry weight (116%), total shoot biomass (136%) resulted in enhanced productivity of ST (291%) and R-A (575%) in plants inoculated with mixture of PSB as compared to the uninoculated plants. The soils of PSB treated plants contained more available P than the soils of uninoculated plants (increase of 86–576%). PSB inoculated plants also recorded higher P content (64–273% increase) compared to uninoculated plants. The PSB strains differed in the extent of rhizosphere colonization, carbon source utilization pattern and whole cell fatty acids methyl esters composition.


Stevia rebaudiana Stevioside Rebaudioside-A Mussoorie rock phosphate Phosphate solubilizing bacteria FAME 


  1. Altomare C, Norvell WA, Bjorkman T, Harman GE (1999) Solubilization of phosphates and micronutrients by plant-growth promoting and biocontrol fungus Trichoderma harzianum Rifai. Appl Environ Microbiol 65:2926–2933PubMedGoogle Scholar
  2. Coenye T, Mahenthiralingam E, Henry D, LiPuma JJ, Laevens S, Gillis M, Speert DP, Vandamme P (2001) Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. Int J Syst Evol Microbiol 51:1481–1490PubMedCrossRefGoogle Scholar
  3. Das K, Dang R (2010) Influence of biofertilizers on stevioside content in Stevia rebaudiana grown in acidic soil condition. Arch Appl Sci Res 4:44–49Google Scholar
  4. de Freitas JR, Banerjee MR, Germida JJ (1997) Phosphate solubilizing rhizobacteria enhance the growth and yield but not phosphorus uptake of canola (Brassica napus L.). Biol Fert Soils 24:358–364CrossRefGoogle Scholar
  5. Ekin Z (2010) Performance of phosphate solubilizing bacteria for improving growth and yield of sunflower (Helianthus annuus L.) in the presence of phosphorus fertilizer. Afr J Biotechnol 25:3794–3800Google Scholar
  6. Ferri LA, Alves-Do-Prado W, Yamada SS, Gazola S, Batista MR, Bazotte RB (2006) Investigation of the antihypertensive effect of oral crude stevioside in patients with mild essential hypertension. Phytother Res 20:732–736PubMedCrossRefGoogle Scholar
  7. Gillis M, Van TV, Bardin R, Goor M, Hebbar P, Willems A, Segers P, Kersters K, Heulin T, Fernandez MP (1995) Polyphasic taxonomy in the genus Burkholderia Leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N-fixing isolates from rice in Vietnam. Int J Syst Evol Bacteriol 45:274–289CrossRefGoogle Scholar
  8. Gulati A, Rahi P, Vyas P, Kasana RC (2009) Plant growth-promoting and rhizosphere-competent Acinetobacter rhizosphaerae Strain BIHB 723 from the cold deserts of Himalayas. Curr Microbiol 58:371–377PubMedCrossRefGoogle Scholar
  9. Jackson ML (1973) Methods of chemical analyses. Prentice Hall of India, New DelhiGoogle Scholar
  10. Jaleel CA, Manivannan P, Sankar B, Kishorekumar A, Gopi R, Somasundaram R, Panneerselvam R (2007) Pseudomonas fluorescens enhances biomass yield and ajmalicine production in Catharanthus roseus under water deficit stress. Colloid Surf. B- Biointerfaces 60:7–11CrossRefGoogle Scholar
  11. Kaymak HC, Yarali F, Guvenc I, Donmez MF (2008) The effect of inoculation with plant growth rhizobacteria (PGPR) on root formation of mint (Mentha piperita L.) cuttings. Afr J Biotechnol 7:4479–4483Google Scholar
  12. Khan KS, Joergensen RG (2009) Changes in microbial biomass and P fractions in biogenic household waste compost amended with inorganic P fertilizers. Bioresource Technol 100:303–309CrossRefGoogle Scholar
  13. Koeing RA, Johnson CR (1942) Colorimetric determination of phosphorus in biological materials. Ind Eng Chem 14:155–156Google Scholar
  14. Kumar KV, Srivastava S, Singh N, Behl HM (2009) Role of metal resistant plant growth promoting bacteria in ameliorating fly ash to the growth of Brassica juncea. J Hazard Mater 170:51–57PubMedCrossRefGoogle Scholar
  15. Li B, Yu R, Liu B, Tang Q, Zhang G, Wang Y, Xie G, Sun G (2011) Characterization and comparison of Serratia marcescens isolated from edible cactus and from silkworm for virulence potential and chitosan susceptibility. Brazilian J Microbiol 42:96–104CrossRefGoogle Scholar
  16. Lugtenberg BJJ, Dekkers L, Bloemberg GV (2001) Molecular determinants of rhizosphere colonization by Pseudomonas. Annu Rev Phytopathol 39:461–490PubMedCrossRefGoogle Scholar
  17. Mamta, Rahi P, Pathania V, Gulati A, Singh B, Bhanwra RK, Tewari R (2010) Stimulatory effect of phosphate-solubilizing bacteria on plant growth, stevioside and rebaudioside-A contents of Stevia rebaudiana Bertoni. Appl Soil Ecol 46:222–229CrossRefGoogle Scholar
  18. Melis MS, Rocha ST, Augusto A (2009) Steviol effect, a glycoside of Stevia rebaudiana on glucose clearances in rats. Brazilian J Biol 69:371–374CrossRefGoogle Scholar
  19. Mittal V, Singh O, Nayyar H, Kaur J, Tewari R (2008) Stimulatory effect of phosphate-solubilizing fungal strains (Aspergillus awamori and Penicillium citrinum) on the yield of chickpea (Cicer arietinum L. cv. GPF2). Soil Biol Biochem 40:718–727CrossRefGoogle Scholar
  20. Olsen SR, Cole CV, Watanabe FS, Dean LA (1954) Estimation of available phosphorous in soils by extraction with sodium bicarbonate. USDA Circ 1:939Google Scholar
  21. Raja P, Uma S, Gopal H, Govindarajan K (2006) Impact of bio inoculants consortium on rice root exudates, biological nitrogen fixation and plant growth. J Biol Sci 6:815–823CrossRefGoogle Scholar
  22. Rascoe J, Berg M, Melcher U, Mitchell FL, Bruton BD, Pair SD, Fletcher J (2003) Identification, phylogenetic analysis, and biological characterization of Serratia marcescens strains causing cucurbit yellow vine disease. Phytopathology 93:1233–1239PubMedCrossRefGoogle Scholar
  23. Sárdi K, Osztoics E, Csathó P, Balázsy A (2009) Correlation between soil P test results and P contents of young spring barley studied in long-term experiments. Comm Soil Sci Plant Anal 40:526–537CrossRefGoogle Scholar
  24. Sasser M, Wichman MD (1991) Identification of microorganisms through use of gas chromatography and high-performance liquid chromatography. In: Hausler WJ Jr, Herrman KL, Isenberg HD, Shadomy HJ (eds) Manual of clinical microbiology. Am Soc Microbiol, Washington, pp 111–118Google Scholar
  25. Schachtman DP, Reid RJ, Ayling SM (1998) Phosphorus uptake by plants: from soil to cell. Plant Physiol 116:447–453PubMedCrossRefGoogle Scholar
  26. Selvakumar G, Mohan M, Kundu S, Gupta AD, Joshi P, Nazim S, Gupta HS (2008) Cold tolerance and plant growth promotion potential of Serratia marcescens strain SRM (MTCC 8708) isolated from flowers of summer squash (Cucurbita pepo). Lett Appl Microbiol 46:171–175PubMedCrossRefGoogle Scholar
  27. Sharma SN, Prasad R (2003) Yield and P uptake by rice and wheat grown in a sequence as influenced by phosphate fertilization with diammonium phosphate and mussoorie rock phosphate with or without crop residue and phosphate solubilizing bacteria. J Agric Sci 141:359–369CrossRefGoogle Scholar
  28. Sharma SN, Prasad R, Shivay YS, Dwivedi MK, Kumar S, Davari MR, Ram M, Kumar D (2010) Relative efficiency of diammonium phosphate and mussoorie phosphate rock on productivity and phosphorus balance in a rice-rapeseed-mungbean cropping system. Nutr Cycl Agroecosyst 86:199–209CrossRefGoogle Scholar
  29. Shivay YS (2010) Effect of diammonium phosphate and mussorrie rock phosphate on productivity and economics of potato (Solanum tuberosum). Indian J Agric Sci 80:329–332Google Scholar
  30. Sulbarán M, Pérez E, Ball MM, Bahsas A, Yarzábal LA (2009) Characterization of the mineral phosphate-solubilizing activity of Pantoea agglomerans MMB051 isolated from an iron-rich soil in southeastern Venezuela (Bolívar State). Curr Microbiol 58:378–383PubMedCrossRefGoogle Scholar
  31. Vassilev N, Vassileva M (2003) Biotechnological solubilization of rock phosphate on media containing agro industrial wastes. Appl Microbiol Biotechnol 61:435–440PubMedGoogle Scholar
  32. Vyas P, Gulati A (2009) Organic acid production in vitro and plant growth promotion in maize under controlled environment by phosphate-solubilizing fluorescent Pseudomonas. BMC Microbiol 9:174PubMedCrossRefGoogle Scholar
  33. Wang GF, Xie GL, Zhu B, Huang JS, Liu B, Kawicha P, Benyon L, Duan YP (2010) Identification and characterization of the Enterobacter complex causing mulberry (Morus alba) wilt disease in China. Eur J Plant Pathol 126:465–478CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Mamta Gupta
    • 1
  • Shashi Bisht
    • 2
  • Bikram Singh
    • 3
  • Arvind Gulati
    • 2
  • Rupinder Tewari
    • 1
  1. 1.Department of Microbial BiotechnologyPanjab University (PU)ChandigarhIndia
  2. 2.Plant Pathology and Microbiology Laboratory, Hill Area Tea Science DivisionInstitute of Himalayan Bioresource Technology (CSIR)PalampurIndia
  3. 3.Department of Natural Plant ProductsInstitute of Himalayan Bioresource Technology (CSIR)PalampurIndia

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