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Plant Growth Regulation

, Volume 63, Issue 1, pp 89–99 | Cite as

Examining the interaction of light, nutrients and carbohydrates on seed germination and early seedling development of Bletia purpurea (Orchidaceae)

  • Timothy R. JohnsonEmail author
  • Michael E. Kane
  • Hector E. Pérez
Original Research

Abstract

The effects of carbohydrate availability, carbohydrate source, nutrient availability and illumination on germination and early development of Bletia purpurea (Orchidaceae) seeds were investigated using asymbiotic seed germination. Of special interest was determining the minimum nutritional and light requirements for the completion of germination. Germination and development was limited when seeds were cultured in darkness without sucrose. Seeds were able to germinate under illuminated conditions even in the absence of sucrose and this effect was enhanced when mineral nutrients were incorporated into media. Sucrose, fructose, glucose and trehalose enhanced germination and seedling development while mannitol and sorbitol did not. These data suggest that carbohydrates, either as products of photosynthesis, from symbiotic fungi in situ or as exogenously supplied sugars in vitro, play an important role in regulating seed germination by fulfilling an energy requirement. This hypothesis has been often expressed but rarely satisfactorily tested. Mineral nutrients appear to be less important for germination than carbohydrates. The differential effect of sucrose, fructose, glucose and trehalose at two different concentrations on rhizoid production indicates carbohydrates may play a role in regulating rhizoid production.

Keywords

Nutrition Photoblastic Rhizoid Seed physiology Seedling development 

Notes

Acknowledgments

The authors thank Dr. Phil Kauth and Nancy Philman (University of Florida; Plant Restoration, Conservation, and Propagation Biotechnology Laboratory) for assistance with the preparation of this manuscript and assistance in the field. The authors also thank Meghan Brennan from the University of Florida department of Statistics in the Institute of Food and Agricultural Sciences for assistance with statistical analysis, as well as Larry Richardson (US Fish and Wildlife Service) for field assistance. This research was supported in part by a grant from the US Fish and Wildlife Service and the Florida Panther National Wildlife Refuge.

References

  1. Alexander C, Hadley G (1985) Carbon movement between host and mycorrhizal endophyte during the development of the orchid Goodyera repens Br. New Phytol 101:657–665CrossRefGoogle Scholar
  2. Alexander C, Alexander IJ, Hadley G (1984) Phosphate uptake by Goodyera repens in relation to mycorrhizal infection. New Phytol 97:401–411CrossRefGoogle Scholar
  3. Arditti J, Michaud JD, Olive AP (1981) Seed germination of North American Orchids. I. Native California and related species of Calypso, Epipactis, Goodyera, Piperia, and Platanthera. Bot Gaz 142:442–453CrossRefGoogle Scholar
  4. Batty AL, Brundrett MC, Dixon KW, Sivasithamparam K (2006) New methods to improve symbiotic propagation of temperate terrestrial orchid seedlings from axenic culture to soil. Aust J Bot 54:367–374CrossRefGoogle Scholar
  5. Bonfil C (1998) The effects of seed size, cotyledon reserves, and herbivory on seedling survival and growth in Quercus rugosa and Q. laurina (Fagaceae). Am J Bot 85:79–87CrossRefGoogle Scholar
  6. Brown PM (2002) Wild orchids of Florida. University Press of Florida, GainesvilleGoogle Scholar
  7. Brundrett M, Scade A, Batty A, Dixon K, Sivasithamparam K (2003) Development of in situ and ex situ seed baiting techniques to detect mycorrhizal fungi from terrestrial orchid habitats. Mycol Res 107:1210–1220CrossRefPubMedGoogle Scholar
  8. Chou LC, Chang DCN (2004) Asymbiotic and symbiotic seed germination of Anoectochilus formosanus and Haemaria discolor and their F1 hybrids. Bot Bull Acad Sin 45:143–147Google Scholar
  9. Coile NC, Garland MA (2003) Notes on Florida’s endangered and threatened plants. Florida Department of Agriculture and Consumer Services, Division of Plant Industry, GainesvilleGoogle Scholar
  10. Correll DS (1978) Native orchids of North America north of Mexico. Stanford University Press, Stanford, CAGoogle Scholar
  11. Dekkers BJW, Schuurmans J, Smeekens SCM (2004) Glucose delays seed germination in Arabidopsis thaliana. Planta 218:579–588CrossRefPubMedGoogle Scholar
  12. Diez JM (2007) Hierarchical patterns of symbiotic orchid germination linked to adult proximity and environmental gradients. J Ecol 95:159–170CrossRefGoogle Scholar
  13. Downie DG (1941) Notes on the germination of some British orchids. Trans Bot Soc Edinb 33:94–103Google Scholar
  14. Dressler RL (1993) Field guide to the orchids of Costa Rica and Panama. Comstock Publishing Associates, IthacaGoogle Scholar
  15. Dutra D, Johnson TR, Kauth PJ, Stewart SL, Kane ME, Richardson L (2008) Asymbiotic seed germination, in vitro seedling development, and greenhouse acclimatization of the threatened terrestrial orchid Bletia purpurea. Plant Cell Tiss Organ Cult 94:11–21CrossRefGoogle Scholar
  16. Dutra D, Kane ME, Richardson L (2009) Asymbiotic seed germination and in vitro seedling development of Cyrtopodium punctatum: a propagation protocol for an endangered Florida native orchid. Plant Cell Tiss Organ Cult 96:235–243CrossRefGoogle Scholar
  17. Ernst R (1967) Effect of carbohydrate selection on the growth rate of freshly germinated Phalaenopsis and Dendrobium seed. Am Orchid Soc Bull 36:1068–1073Google Scholar
  18. Ernst R, Arditti J (1990) Carbohydrate physiology of orchid seedlings III. Hydrolysis of maltooligosaccharides by Phalaenopsis (Orchidaceae) seedlings. Am J Bot 77:188–195CrossRefGoogle Scholar
  19. Ernst R, Arditti J, Healey PL (1971) Carbohydrate physiology of orchid seedlings II. Hydrolysis and effects of oligosaccharides. Am J Bot 58:827–835CrossRefGoogle Scholar
  20. Finkelstein RR, Lynch TJ (2000) Abscisic acid inhibition of radicle emergence but not seedling growth is suppressed by sugars. Plant Physiol 122:1179–1186CrossRefPubMedGoogle Scholar
  21. Fukai S, Fujiwara K, Okamoto K, Hasegawa A, Goi M (1997) Effects of red and blue light on germination and protocorm growth of Calanthe Satsuma. Lindleyana 12:169–171Google Scholar
  22. Gayatri M, Kavyashree R (2007) Effect of carbon source on in vitro seed germination of monopodial orchid—Epidendrum radicans. Phytomorphology 57:67–70Google Scholar
  23. Górecki R, Brenac P, Clapham W, Willcott J, Obendorf R (1996) Soluble carbohydrates in white lupin seeds matured at 13 and 28°C. Crop Sci 36:1277–1282CrossRefGoogle Scholar
  24. Harvais G (1974) Notes on the biology of some native orchids of Thunder Bay, their endophytes and symbionts. Can J Bot 52:451–460CrossRefGoogle Scholar
  25. Johnson TR (2007) Asymbiotic seed germination of Vanda: in vitro germination and development of three hybrids. University of Florida, GainesvilleGoogle Scholar
  26. Johnson TR, Stewart SL, Dutra D, Kane ME, Richardson L (2007) Asymbiotic and symbiotic seed germination of Eulophia alta (Orchidaceae)—preliminary evidence for the symbiotic culture advantage. Plant Cell Tiss Organ Cult 90:313–323CrossRefGoogle Scholar
  27. Kauth PJ (pers. comm.) In: Editor (ed) BookGoogle Scholar
  28. Karrer E, Rodriguez R (1992) Metabolic regulation of rice α-amylase and sucrose synthase genes in planta. Plant J 2:517–523PubMedGoogle Scholar
  29. Kauth PJ, Vendrame WA, Kane ME (2006) In vitro seed culture and seedling development of Calopogon tuberosus. Plant Cell Tiss Organ Cult 85:91–102CrossRefGoogle Scholar
  30. Kauth PJ, Kane ME, Vendrame WA, Reinhardt-Adams C (2008) Asymbiotic germination response to photoperiod and nutritional media in six populations of Calopogon tuberosus var. tuberosus (Orchidaceae): evidence for ecotypic differentiation. Ann Bot 102:783–793CrossRefPubMedGoogle Scholar
  31. Kitajima K (2003) Impact of cotyledon and leaf removal on seedling survival in three tree species with contrasting cotyledon functions. Biotropica 35:429–434Google Scholar
  32. Knudson L (1922) Nonsymbiotic germination of orchid seeds. Bot Gaz 73:1–25CrossRefGoogle Scholar
  33. Kuo T, Doehlert D, Carwfor C (1990) Sugar metabolism in germinating soybean seeds. Plant Physiol 93:1514–1520CrossRefPubMedGoogle Scholar
  34. Leroux G, Barabe D, Vieth J (1995) Comparative morphogenesis of Cypripedium acaule (Orchidaeceae) protocorms cultivated in vitro with or without sugar. Can J Bot 73:1391–1406CrossRefGoogle Scholar
  35. Lo SF, Nalawade SM, Kuo CL, Chen CL, Tsay HS (2004) Asymbiotic germination of immature seeds, plantlet development and ex vitro establishment of plants of Dendrobium tosaense Makino—a medicinally important orchid. In Vitro Cell Dev Biol Plant 40:528–535CrossRefGoogle Scholar
  36. Manning JC, van Staden J (1987) The development and mobilization of seed reserves in some African orchids. Aust J Bot 35:343–353CrossRefGoogle Scholar
  37. McKendrick SL, Leake JR, Read DJ (2000) Symbiotic germination and development of myco-heterotrophic plants in nature: transfer of carbon from ectomycorrhizal Salix repens and Betula pendula to the orchid Corallorhiza trifida through shared hyphal connections. New Phytol 145:539–548CrossRefGoogle Scholar
  38. McKinley TC, Camper ND (1997) Action spectra of in vitro asymbiotic germination of Goodyera repens var. ophioides. Lindleyana 12:30–33Google Scholar
  39. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  40. Obendorf R, Zimmerman A, Zhang Q, Castillo A, Kosina S, Bryant E, Sensenig E, Wu J, Schnebly S (2009) Accumulation of soluble carbohydrates during seed development and maturation of low-raffinose, low-stachyose soybean. Crop Sci 49:329–341CrossRefGoogle Scholar
  41. Øien D-I, O’Neill J, Whigham DF, McCormick MK (2008) Germination ecology of the boreal-alpine terrestrial orchid Dactylorhiza lapponica (Orchidaceae). Ann Bot Fenn 45:161–172Google Scholar
  42. Pan MJ, van Staden J (1999) Effect of activated charcoal, autoclaving and culture media on sucrose hydrolysis. Plant Growth Regul 29:135–141CrossRefGoogle Scholar
  43. Penfield S, King J (2009) Towards a systems biology approach to understanding dormancy and germination. Proc R Soc Lond B 276:3561–3569CrossRefGoogle Scholar
  44. Perata P, Matsukura C, Vernieri P, Yamaguchi J (1997) Sugar repression of a gibberellin-dependent signaling pathway in barley embryos. Plant Cell 9:2197–2208CrossRefPubMedGoogle Scholar
  45. Price J, Li T, Kang S, Na J, Jang J (2003) Mechanisms of glucose signaling during germination of Arabidopsis. Plant Physiol 132:1424–1438CrossRefPubMedGoogle Scholar
  46. Pritchard HW, Poynter ALC, Seaton PT (1999) Interspecies variation in orchid seed longevity in relation to ultra-dry storage and cryopreservation. Lindleyana 14:92–101Google Scholar
  47. Purves S, Hadley G (1976) Physiology of symbiosis in Goodyera repens. New Phytol 77:689–696CrossRefGoogle Scholar
  48. Rasmussen HN (1995) Terrestrial orchids: from seed to mycotrophic plant. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  49. Rasmussen H, Rasmussen FN (1991) Climatic and seasonal regulation of seed plant establishment in Dactylorhiza majalis inferred from symbiotic experiments in vitro. Lindleyana 6:221–227Google Scholar
  50. Rasmussen H, Whigham DF (1998) Importance of woody debris in seed germination of Tipularia discolor (Orchidaceae). Am J Bot 85:829–834CrossRefGoogle Scholar
  51. Rasmussen H, Andersen TF, Johansen B (1990a) Light stimulation and darkness requirement for the symbiotic germination of Dactylorhiza majalis (Orchidaceae) in vitro. Physiol Plant 79:226–230CrossRefGoogle Scholar
  52. Rasmussen H, Andersen TF, Johansen B (1990b) Temperature sensitivity of in vitro germination and seedling development of Dactylorhiza majalis (Orchidaceae) with and without a mycorrhizal fungus. Plant Cell Environ 13:171–177CrossRefGoogle Scholar
  53. Richardson KA, Peterson RL, Currah RS (1992) Seed reserves and early symbiotic protocorm development of Platanthera hyperborea (Orchidaceae). Can J Bot 70:291–300CrossRefGoogle Scholar
  54. Sawyer H, Hsiao KC (1992) Effects of autoclaving-induced carbohydrate hydrolysis on the growth of Beta vulgaris cells in suspension. Plant Cell Tiss Organ Cult 31:81–86Google Scholar
  55. Schenk N, Hsiao KC, Bornman CH (1991) Avoidance of precipitation and carbohydrate breakdown in autoclaved plant tissue culture media. Plant Cell Rep 10:115–119CrossRefGoogle Scholar
  56. Shimura H, Koda Y (2005) Enhanced symbiotic seed germination of Cypripedium macranthos var. rebunense following inoculation after cold treatment. Physiol Plant 123:281–287CrossRefGoogle Scholar
  57. Short TW (1999) Overexpression of Arabidopsis phytochrome B inhibits phytochrome A function in the presence of sucrose. Plant Physiol 119:1497–1505CrossRefPubMedGoogle Scholar
  58. Smith SE (1973) Asymbiotic germination of orchid seeds on carbohydrates of fungal origin. New Phytol 72:497–499CrossRefGoogle Scholar
  59. Stewart SL (2006) Symbiotic orchid seed germination techniques. In: Hicks AJ (ed) Asymbiotic techniques of orchid seed germination, 2nd edn. Orchid Seedbank Project, ChandlerGoogle Scholar
  60. Stewart SL, Kane ME (2006a) Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tiss Organ Cult 86:147–158CrossRefGoogle Scholar
  61. Stewart SL, Kane ME (2006b) Symbiotic seed germination of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tiss Organ Cult 86:159–167CrossRefGoogle Scholar
  62. Stewart SL, Kane ME (2007) Symbiotic seed germination and evidence for in vitro mycobiont specificity in Spiranthes brevilabris (Orchidaceae) and its implications for species-level conservation. In Vitro Cell Dev Biol Plant 43:178–186CrossRefGoogle Scholar
  63. Stewart SL, Kane ME (2010) Effect of carbohydrate source on the in vitro asymbiotic seed germination of the terrestrial orchid Habenaria macroceratitis. J Plant Nutr 33:1–11CrossRefGoogle Scholar
  64. Stewart SL, Zettler LW (2002) Symbiotic germination of three semi-aquatic rein orchids (Habenaria repens, H. quinquiseta, H. macroceratitis) from Florida. Aquat Bot 72:25–35CrossRefGoogle Scholar
  65. Stoutamire WP (1964) Seeds and seedlings of native orchids. Mich Bot 3:107–119Google Scholar
  66. Stoutamire WP (1974) Terrestrial orchid seedlings. In: Withner C (ed) The orchids: scientific studies. Robert E. Krieger Publishing Company, Inc., Malabar, pp 101–128Google Scholar
  67. Tsutsui K, Tomita M (1990) Suitability of several carbohydrates as the carbon sources for symbiotic growth of two orchid species. Lindleyana 5:134–139Google Scholar
  68. Van Waes JM, Debergh PC (1986) In vitro germination of some Western European orchids. Physiol Plant 67:253–261CrossRefGoogle Scholar
  69. Vasudevan R, Van Staden J (2010) In vitro asymbiotic seed germination and seedling growth of Ansellia africana Lindl. Sci Hortic 123:496–504CrossRefGoogle Scholar
  70. Vermeulen P (1947) Studies on dactylorchids. Schotanus and Jens, UtretchGoogle Scholar
  71. Wang H, Deng X (2002) Phytochrome signaling mechanism. In: Somerville CR, Meyerowitz EM (eds) The Arabidopsis book. American Society of Plant Biologists, RockvilleGoogle Scholar
  72. Wang XJ, Hsiao KC (1995) Sugar degradation during autoclaving: effects of duration and solution volume on breakdown of glucose. Physiol Plant 94:415–418CrossRefGoogle Scholar
  73. Ward DB, Austin DF, Coile NC (2003) Endangered and threatened plants of Florida, ranked in order of rarity. Castanea 68:160–174Google Scholar
  74. Williams LO, Allen PH (1998) Orchids of Panama. Missouri Botanical Garden Press, St. LouisGoogle Scholar
  75. Wynd FL (1933) Sources of carbohydrate for germination and growth of orchid seedlings. Ann Mo Bot Gard 20:569–581CrossRefGoogle Scholar
  76. Yagame T, Yamato M, Mii M, Suzuki A, Iwase K (2007) Developmental processes of achlorophyllous orchid, Epipogium roseum: from seed germination to flowering under symbiotic cultivation with mycorrhizal fungus. J Plant Res 120:229–236CrossRefPubMedGoogle Scholar
  77. Yeung EC, Law SK (1992) Embryology of Calypso bulbosa. II. Embryo development. Can J Bot 70:461–468CrossRefGoogle Scholar
  78. Yoder JA, Zettler LW, Stewart SL (2000) Water requirements of terrestrial and epiphytic orchid seeds and seedlings, and evidence for water uptake by means of mycotrophy. Plant Sci 156:145–150CrossRefPubMedGoogle Scholar
  79. Young TE, Juvik JA, DeMason DA (1997) Changes in carbohydrate composition and α-amylase expression during germination and seedling growth of starch-deficient endosperm mutants of maize. Plant Sci 129:175–189CrossRefGoogle Scholar
  80. Yuan K, Wysocka-Diller J (2006) Phytohormone signalling pathways interact with sugars during seed germination and seedling development. J Exp Biol 57:3359–3367Google Scholar
  81. Zettler LW, McInnis TM (1994) Light enhancement of symbiotic seed germination and development of an endangered terrestrial orchid (Platanthera integrilabia). Plant Sci 102:133–138CrossRefGoogle Scholar
  82. Zettler LW, Perlman S, Dennis DJ, Hopkins SE, Poulter SB (2005) Symbiotic germination of a federally endangered Hawaiian endemic, Platanthera holochila (Orchidaceae), using a mycobiont from Florida: a conservation dilemma. Selbyana 26:269–276Google Scholar
  83. Zettler LW, Poulter SB, McDonald KI, Stewart SL (2007) Conservation-driven propagation of an epiphytic orchid (Epidendrum nocturnum) with a mycorrhizal fungus. Hortscience 42:135–139Google Scholar
  84. Zhao M, Liu R, Chen L, Tian Q, Zhang W (2009) Glucos-induced inhibition of seed germination in Lotus japonicus is alleviated by nitric oxide and spermine. J Plant Physiol 166:213–218CrossRefPubMedGoogle Scholar
  85. Zhu G, Ye N, Zhang J (2009) Glucose-induced delay of seed germination in rice is mediated by the suppression of ABA catabolism rather than an enhancement of ABA biosynthesis. Plant Cell Physiol 50:644–651CrossRefPubMedGoogle Scholar
  86. Znaniecka J, Krolicka A, Sidwa-Gorycka M, Rybczynski JJ, Szlachetko DL, Lojkowska E (2005) Asymbiotic germination, seedling development and plantlet propagation of Encyclia aff. oncidioides—an endangered orchid. Acta Soc Bot Pol 74:193–198Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Timothy R. Johnson
    • 1
    • 2
    Email author
  • Michael E. Kane
    • 1
    • 2
  • Hector E. Pérez
    • 2
  1. 1.Plant Restoration, Conservation & Propagation Biotechnology Laboratory, Department of Environmental HorticultureUniversity of FloridaGainesvilleUSA
  2. 2.Plant Restoration and Conservation Horticulture Consortium, Department of Environmental HorticultureUniversity of FloridaGainesvilleUSA

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