Proteome expression patterns in the stress tolerant evergreen Ammopiptanthus nanus under conditions of extreme cold
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Low temperature is one of the important environmental changes that affect plant growth. The cold resistance capabilities of evergreen plants are the result of long-term adaptation to extreme environmental conditions. To investigate the responses of Ammopiptanthus nanus, a rare stress-tolerant evergreen plant, to extreme cold stress, we analyzed the proteome expression patterns of stressed plants; this is the first study to report these patterns for A. nanus. We collected adult A. nanus leaves under two conditions of cold stress: extreme cold (−29°C) and relatively less extreme cold (−5°C). Total crude proteins were extracted from leaf blades, separated by two-dimensional gel electrophoresis, and stained with Coomassie brilliant blue. Of the 500 protein spots detected in each of the samples, eight of the spots that exhibited clear changes under the different conditions were identified by MALDI-TOF analyses. Our results suggest that cold stress-related proteins may play diverse roles in the resistance to multiple environmental stresses.
KeywordsExtreme cold conditions Two-dimensional gel electrophoresis Ammopiptanthus nanus (Popov) Proteome analysis
This work was supported by Basic Research Program of Ministry of Science and Technology of China (2004CCA03400), Natural Science Foundation of Xinjiang province (200821160) and Particular Projects for Preservation and Sustainable Utilization of Strategic Resources of the Chinese Academy of Science (200731138).
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