Plant Growth Regulation

, Volume 61, Issue 3, pp 231–242 | Cite as

Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants

  • Somya Dwivedi
  • Radomira Vanková
  • Vaclav Motyka
  • Carmen Herrera
  • Eva Zizkova
  • Carol Auer
Original Research

Abstract

Cytokinin analogue roscovitine exhibits a strong inhibitory effect on cytokinin N-glucosylation, one of the most important pathways of cytokinin inactivation in plants. Roscovitine-resistant mutant. (ror-1) was isolated using T-DNA tagged lines of Arabidopsis thaliana (L.) Heynh in order to find a gene putatively involved in cytokinin N-glucosylation. The amount of cytokinin N-glucosides of trans-zeatin- and isopentenyladenine-type was elevated by 20% in ror-1 mutant compared to WT. The cytokinin oxidase/dehydrogenase activity exhibited a mild elevation in ror-1 compared to WT in basal media. Additionally, ror-1 plants showed slightly enhanced resistance to exogenously supplied aromatic cytokinins (benzyladenine). Incubation with exogenous cytokinin (5 μM BA for 24 h) resulted in significant up-regulation of ROR-1 gene expression in ror-1 mutant. In silico analysis showed that ROR-1 gene encoded for a protein consisting of GRAM (Glycosyltransferases Rab-like GTPase activators and Myotubularins) and C2 domains. Here, we report on the role of ROR-1 gene in metabolism of bioactive cytokinins in the plants.

Keywords

Cytokinin metabolism N-Glucosyltransferase GRAM domain Aromatic cytokinins Roscovitine 

Abbreviations

ABA

Abscisic acid

BA

N6-Benzyladenine

cisZ

cis-Zeatin

cisZR

cis-Zeatin 9-riboside

cisZRMP

cis-Zeatin 9-riboside-5′-monophosphate

cisZOG

cis-Zeatin O-glucoside

cisZ7G

cis-Zeatin 7-glucoside

cisZ9G

cis-Zeatin 9-glucoside

CKX

Cytokinin oxidase/dehydrogenase

DHZ

Dihydrozeatin

DHZR

Dihydrozeatin 9-riboside

DHZRMP

Dihydrozeatin 9-riboside-5′-monophosphate

DHZOG

Dihydrozeatin O-glucoside

DHZ7G

Dihydrozeatin 7-glucoside

DHZ9G

Dihydrozeatin 9-glucoside

GRAM

Glycosyltransferases Rab-like GTPase activators and Myotubularins

iP

N6-(Δ2-Isopentenyl)adenine

iPR

N6-(Δ2-Isopentenyl)adenine 9-riboside

iPRMP

N6-(Δ2-Isopentenyl)adenine 9-riboside-5′-monophosphate

iP7G

N6-(Δ2-Isopentenyl)adenine 7-glucoside

iP9G

N6-(Δ2-Isopentenyl)adenine 9-glucoside

Z

trans-Zeatin

ZR

trans-Zeatin 9-riboside

ZRMP

trans-Zeatin 9-riboside-5′-monophosphate

ZOG

trans-Zeatin O-glucoside

Z7G

trans-Zeatin 7-glucoside

Z9G

trans-Zeatin 9-glucoside

Notes

Acknowledgments

The authors wish to thank Dr. Mary Musgrave for her guidance in editing this manuscript, Dr. Petre Dobrev and Dr. Jiří Malbeck (both Institute of Experimental Botany AS CR, Prague, Czech Republic) for phytohormone analyses and Marie Korecka (Institute of Experimental Botany AS CR, Prague, Czech Republic) for excellent technical assistance. This research was financially supported by the NSF International Programs for Central and Eastern Europe and by Hatch funds administered through the Storrs Agricultural Experiment Station at the University of Connecticut, Storrs (S.D., C.H., C.A.) and by the Grant Agency of the Academy of Sciences of the Czech Republic (IAA600380701) and by the Ministry of Education Youth and Sports of the Czech Republic (LC06034 and KONTAKT ME505; R.V., V.M., E.Z.).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Somya Dwivedi
    • 1
  • Radomira Vanková
    • 2
  • Vaclav Motyka
    • 2
  • Carmen Herrera
    • 3
  • Eva Zizkova
    • 2
    • 4
  • Carol Auer
    • 3
  1. 1.Department of BiologyPennsylvania State University-York CampusYorkUSA
  2. 2.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicPrague 6Czech Republic
  3. 3.Department of Plant ScienceUniversity of ConnecticutStorrsUSA
  4. 4.Department of Plant Physiology, Faculty of ScienceCharles UniversityPrague 2Czech Republic

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