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Plant Growth Regulation

, Volume 61, Issue 1, pp 45–52 | Cite as

Silicon alleviates cadmium toxicity in peanut plants in relation to cadmium distribution and stimulation of antioxidative enzymes

  • Gangrong Shi
  • Qingsheng Cai
  • Caifeng Liu
  • Li Wu
Original Paper

Abstract

Silicon (Si) is generally considered a beneficial element for the growth of higher plants, especially for those grown under stressed environments. Recently, the mitigating role of Si in cadmium (Cd) stress has received some attention. However, its mechanisms involved remain poorly understood. We studied the effects of Si on tissue and subcellular distribution of Cd, as well as the activities of major antioxidant enzymes (SOD, POD and CAT) with two contrasting peanut (Arachis hypogaea L.) cultivars (Luhua 11 and Luzi 101) differing in their Cd tolerance. The results showed that Cd exposure alone depressed plant growth and caused oxidative stress for both cultivars, and this toxicity was more obvious in Cd-sensitive cultivar (Luhua 11) than in Cd-tolerant cultivar (Luzi 101). Si supply significantly alleviated the toxicity of Cd in peanut seedlings; this was correlated with a reduction of shoot Cd accumulation, an alteration of Cd subcellular distribution in leaves, and a stimulation of antioxidative enzymes. The mechanisms of Si amelioration of Cd stress were cultivar and tissue dependent. For Luhua 11, Si-mediated inhibition of Cd transport from roots to shoots, reduction of Cd content in cell organelle fractions of leaves, and enhancement of the SOD, POD and CAT activities in roots, might responsible for the role of Si in alleviating Cd toxicity. For Luzi 101, Si alleviation of Cd toxicity is mainly attributed to the decrease in Cd concentration in shoot and stimulation of antioxidants systems.

Keywords

Antioxidant enzyme Arachis hypogaea Cadmium Silicon Subcellular distribution 

Abbreviations

AAS

Atomic absorbance spectrometry

CAT

Catalase

MDA

Malondialdehyde

POD

Peroxidases

ROS

Reactive oxygen species

SOD

Superoxide dismutase

TF

Translocation factor

Notes

Acknowledgements

Financial support from the National Natural Science Foundation of China (No. 40971296) and the Natural Science Foundation for College of Anhui Province (KJ2009B073) is gratefully acknowledged.

References

  1. Aebi H (1984) Catalase in vitro. Meth Enzymol 105:121–126CrossRefPubMedGoogle Scholar
  2. Al-Aghabary K, Zhu Z, Shi Q (2005) Influence of silicon supply on chlorophyll content, chlorophyll fluorescence, and antioxidative enzyme activities in tomato plants under salt stress. J Plant Nutr 27:2101–2115CrossRefGoogle Scholar
  3. Ali NA, Bernal MP, Ater M (2002) Tolerance and bioaccumulation of copper in Phragmites australis and Zea mays. Plant Soil 239:103–111CrossRefGoogle Scholar
  4. Baker AJM (1987) Metal tolerance. New Phytol 106:93–111Google Scholar
  5. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287CrossRefPubMedGoogle Scholar
  6. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254CrossRefPubMedGoogle Scholar
  7. Chen HM, Zheng CR, Tu C, Shen ZG (2000) Chemical methods and phytoremediation of soil contaminated with heavy metals. Chemosphere 41:229–234CrossRefPubMedGoogle Scholar
  8. Clemens S (2001) Molecular mechanisms of plant metal tolerance and homeostasis. Planta 212:475–486CrossRefPubMedGoogle Scholar
  9. da Cunha KPV, do Nascimento CWA (2009) Silicon effects on metal tolerance and structural changes in maize (Zea mays L.) grown on a cadmium and zinc enriched soil. Water Air Soil Poll 197:323–330CrossRefGoogle Scholar
  10. Ekmekçi Y, Tanyolaç D, Ayhan B (2008) Effects of cadmium on antioxidant enzyme and photosynthetic activities in leaves of two maize cultivars. J Plant Physiol 165:600–611CrossRefPubMedGoogle Scholar
  11. Feng J, Shi Q, Wang X, Wei M, Yang F, Xu H (2009) Silicon supplementation ameliorated the inhibition of photosynthesis and nitrate metabolism by cadmium (Cd) toxicity in Cucumis sativus L. Sci Hortic. doi: 10.1016/j.scienta.2009.10.013
  12. Foyer C, Noctor G (2005) Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ 28:1056–1071CrossRefGoogle Scholar
  13. Gong H, Zhu X, Chen K, Wang S, Zhang C (2005) Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Sci 169:313–321CrossRefGoogle Scholar
  14. Gunes A, Inal A, Bagci EG, Coban S, Pilbeam DJ (2007) Silicon mediates changes to some physiological and enzymatic parameters symptomatic for oxidative stress in spinach (Spinacia oleracea L.) grown under B toxicity. Sci Hortic 113:113–119CrossRefGoogle Scholar
  15. Krantev A, Yordanova R, Janda T, Szalai G, Popova L (2008) Treatment with salicylic acid decreases the effect of cadmium on photosynthesis in maize plants. J Plant Physiol 165:920–931CrossRefPubMedGoogle Scholar
  16. Laspina NV, Groppa MD, Tomaro ML, Benavides MP (2005) Nitric oxide protects sun flower leaves against Cd-induced oxidative stress. Plant Sci 169:323–330CrossRefGoogle Scholar
  17. Li HS (2000) Principles and techniques of plant physiological biochemical experiment. Higher Education Press, Beijing, pp 260–263Google Scholar
  18. Liang Y, Yang C, Shi H (2001) Effects of silicon on growth and mineral composition of barley grown under toxic levels of aluminum. J Plant Nutr 24:229–243CrossRefGoogle Scholar
  19. Liang Y, Wong J, Wei L (2005) Silicon-mediated enhancement of cadmium tolerance in maize (Zea mays L.) grown in cadmium contaminated soil. Chemosphere 58:475–483CrossRefPubMedGoogle Scholar
  20. Lozano-Rodriguez E, Hernàndez LE, Bonay P, Carpena-Ruiz RO (1997) Distribution of cadmium in shoot and root tissues of maize and pea plants: physiological disturbances. J Exp Bot 48:123–128CrossRefGoogle Scholar
  21. Ma JF (2004) Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses. Soil Sci Plant Nutr 50:11–18Google Scholar
  22. Moya J, Ros R, Picazo I (1993) Influence of cadmium and nickel on growth, net photosynthesis and carbohydrate distribution in rice plants. Photosynth Res 36:75–80CrossRefGoogle Scholar
  23. Neumann D, zur Nieden U (2001) Silicon and heavy metal tolerance of higher plants. Phytochemistry 56:685–692CrossRefPubMedGoogle Scholar
  24. Nowakowski W, Nowakowska J (1997) Silicon and copper interaction in the growth of spring wheat seedlings. Biol Plant 39:463–466CrossRefGoogle Scholar
  25. Putter J (1974) Peroxidases. In: Bergmeyer HU (ed) Methods of enzymatic analysis: II. Academic Press, New York, pp 685–690Google Scholar
  26. Richmond KE, Sussman M (2003) Got silicon? The non-essential beneficial plant nutrient. Curr Opin Plant Biol 6:268–272CrossRefPubMedGoogle Scholar
  27. Rogalla H, Romheld V (2002) Role of leaf apoplast in silicon-mediated manganese tolerance of Cucumis sativus L. Plant Cell Environ 25:549–555CrossRefGoogle Scholar
  28. Romero-Puertas MC, Rodriguez-Serrano M, Corpas FJ, Gomez M, Del Rio LA, Sandalio LM (2004) Cadmium-induced subcellular accumulation of O2 and H2O2 in pea leaves. Plant Cell Environ 27:1122–1134CrossRefGoogle Scholar
  29. Sandalio L, Dalurzo H, Gomez M, Romero-Puertas M, del Rio L (2001) Cadmium-induced changes in the growth and oxidative metabolism of pea plants. J Exp Bot 52:2115–2126PubMedGoogle Scholar
  30. Shi Q, Bao Z, Zhu Z, He Y, Qian Q, Yu J (2005a) Silicon-mediated alleviation of Mn toxicity in Cucumis sativus in relation to activities of superoxide dismutase and ascorbate peroxidase. Phytochemistry 66:1551–1559CrossRefPubMedGoogle Scholar
  31. Shi X, Zhang C, Wang H, Zhang F (2005b) Effect of Si on the distribution of Cd in rice seedlings. Plant Soil 272:53–60CrossRefGoogle Scholar
  32. Song A, Li Z, Zhang J, Xue G, Fan F, Liang Y (2009) Silicon-enhanced resistance to cadmium toxicity in Brassica chinensis L. is attributed to Si-suppressed cadmium uptake and transport and Si-enhanced antioxidant defense capacity. J Hazard Mater. doi: 10.1016/j.jhazmat.2009.06.143
  33. Sudhakar C, Lakshmi A, Giridarakumar S (2001) Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Sci 161:613–619CrossRefGoogle Scholar
  34. Tiryakioglu M, Eker S, Ozkutlu F, Husted S, Cakmak I (2006) Antioxidant defense system and cadmium uptake in barley genotypes differing in cadmium tolerance. J Trace Elem Med Biol 20:181–189CrossRefPubMedGoogle Scholar
  35. Vaculík M, Lux A, Luxová M, Tanimoto E, Lichtscheidl I (2009) Silicon mitigates cadmium inhibitory effects in young maize plants. Environ Exp Bot 67:52–58CrossRefGoogle Scholar
  36. Van Assche F, Clijsters H (1990) Effects of heavy metals on enzyme activity in plants. Plant Cell Environ 13:195–206CrossRefGoogle Scholar
  37. Wang LJ, Wang YH, Chen Q, Cao WD, Li M, Zhang FS (2000) Silicon induced cadmium tolerance of rice seedlings. J Plant Nutr 23:1397–1406CrossRefGoogle Scholar
  38. Weigel HJ, Jäger HJ (1980) Subcellular distribution and chemical form of cadmium in bean plants. Plant Physiol 65:480–482CrossRefPubMedGoogle Scholar
  39. Yang X, Long X, Ye H, He Z, Calvert D, Stoffella P (2004) Cadmium tolerance and hyperaccumulation in a new Zn-hyperaccumulating plant species (Sedum alfredii Hance). Plant Soil 259:181–189CrossRefGoogle Scholar
  40. Zhang C, Wang L, Nie Q, Zhang W, Zhang F (2008) Long-term effects of exogenous silicon on cadmium translocation and toxicity in rice (Oryza sativa L.). Environ Exp Bot 62:300–307CrossRefGoogle Scholar
  41. Zhu Z, Wei G, Li J, Qian Q, Yu J (2004) Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.). Plant Sci 167:527–533CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Gangrong Shi
    • 1
    • 2
  • Qingsheng Cai
    • 1
  • Caifeng Liu
    • 2
  • Li Wu
    • 2
  1. 1.College of Life SciencesNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.The Provincial Key Laboratory of the Resource Plant Biology in Department of BiologyHuaibei Coal Industry Teachers CollegeHuaibeiPeople’s Republic of China

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