Plant Growth Regulation

, Volume 60, Issue 2, pp 133–141 | Cite as

Effect of plant growth regulators on the biosynthesis of vinblastine, vindoline and catharanthine in Catharanthus roseus

  • Qifang Pan
  • Yu Chen
  • Quan Wang
  • Fang Yuan
  • Shihai Xing
  • Yuesheng Tian
  • Jingya Zhao
  • Xiaofen Sun
  • Kexuan Tang
Original Paper

Abstract

Catharanthuse roseus is a well-known medicinal plant for its two valuable anticancer compounds: vinblastine and vincristine, which belongs to terpenoid indole alkaloids. Great efforts have been made to study the principles of its secondary metabolic pathways to regulate the alkaloids biosynthesis. In this article, different plant growth regulators were shortly applied to Catharanthus roseus plants during the blooming period to study their effects on the biosynthesis of vinblastine, vindoline and catharanthine. Salicylic acid and ethylene (ethephon) treatments resulted in a significant increase of vinblastine, vindoline and catharanthine while abscisic acid and gibberellic acid had a strongly negative influence on the accumulation of the three important alkaloids. Methyl jasmonate showed no great effect on the production of these valuable alkaloids. Chlormequat chloride highly enhanced the accumulation of vinblastine but greatly decreased the contents of vindoline and catharanthine.

Keywords

Catharanthus roseus Plant growth regulators Vinblastine Vindoline Catharanthine Blooming period 

Abbreviations

C. roseus

Catharanthus roseus

PGRs

Plant growth regulators

TIA

Terpenoid indole alkaloids

MeJA

Methyljasmonate

SA

Salicylic acid

GA3

Gibberellic acid

ABA

Abscissic acid

CCC

Chlormequat chloride

AVLBS

3′, 4′-anhydrovinblastine synthase

DMRT

Duncan’s multiple range test

ANOVA

Analysis of variance

Notes

Acknowledgments

This research was supported by China National High-Tech “863” Program (grant number 2007AA10Z189), China “973” Program (grant number 2007CB108805), Shanghai Science and Technology Committee (grant number 08391911800) and Shanghai Leading Academic Discipline Project (Project Number B209).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Qifang Pan
    • 1
  • Yu Chen
    • 1
  • Quan Wang
    • 1
  • Fang Yuan
    • 1
  • Shihai Xing
    • 1
  • Yuesheng Tian
    • 1
  • Jingya Zhao
    • 1
  • Xiaofen Sun
    • 1
  • Kexuan Tang
    • 1
  1. 1.Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and BiologyShanghai Jiao Tong UniversityMinhang District, ShanghaiChina

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