Plant Growth Regulation

, Volume 59, Issue 3, pp 207–214 | Cite as

Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana

  • Yajie Liu
  • Zhiguang Zhao
  • Jing Si
  • Cuixia Di
  • Jin Han
  • Lizhe An
Original Paper

Abstract

Brassinosteroids (BRs), a class of plant steroid hormones, play a significant role in the amelioration of various biotic and abiotic stresses. In order to further explore and elaborate their roles in plants subjected to chilling stress, suspension cultured cells of Chorispora bungeana with or without 24-epibrassinolide (EBR) application were exposed to 4 and 0°C for 5 days. The EBR treated cells exhibited higher viability after exposure to low temperatures compared with the control. Under chilling stress, reactive oxygen species (ROS) levels and lipid peroxidation were increased in the cultured cells, which were significantly inhibited by EBR application. The activities of antioxidative enzymes such as ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) were increased during chilling treatments, and these increases were more significant in the EBR applied suspension cells. The EBR treatment also greatly enhanced contents of ascorbic acid (AsA) and reduced glutathione (GSH) under chilling stress. From these results, it can be concluded that EBR could play the positive roles in the alleviation of oxidative damage caused by ROS overproduction through enhancing antioxidant defense system, resulting in improving the tolerance of C. bungeana suspension cultures to chilling stress.

Keywords

Antioxidant Antioxidative enzymes Brassinosteroids Chilling stress Chorispora bungeana 

Abbreviations

APX

Ascorbate peroxidase

AsA

Ascorbic acid or ascorbate

BRs

Brassinosteroids

CAT

Catalase

EBR

24-Epibrassinolide

GSH

Reduced glutathione

H2O2

Hydrogen peroxide

MDA

Malondialdehyde

NBT

Nitroblue tetrazolium

OH

Hydroxyl radical

O2

Superoxide radical

POD

Peroxidase

ROS

Reactive oxygen species

SOD

Superoxide dismutase

TBA

Thiobarbituric acid

TCA

Trichloroacetic acid

TTC

2, 3, 5-Triphenyltetrazolium chloride

Notes

Acknowledgments

This study has been supported by the National Outstanding Youth Foundation of China (No. 30625008), the National High Technology Research and Development Program of China (863 Program) (No. 2007AA021401) and the National Basic Research Program of China (973 Program) (No. 2007CB108902).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Yajie Liu
    • 1
  • Zhiguang Zhao
    • 1
  • Jing Si
    • 1
  • Cuixia Di
    • 1
  • Jin Han
    • 1
  • Lizhe An
    • 1
  1. 1.State Key Laboratory of Arid AgroecologyLanzhou UniversityLanzhouPeople’s Republic of China

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