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Plant Growth Regulation

, Volume 58, Issue 3, pp 251–260 | Cite as

Sugars induce anthocyanin accumulation and flavanone 3-hydroxylase expression in grape berries

  • Yanjun Zheng
  • Li Tian
  • Hongtao Liu
  • Qiuhong Pan
  • Jicheng Zhan
  • Weidong Huang
Original Paper

Abstract

Flavanone 3-hydroxylase (EC 1.14.11.9, F3H) plays a key role in anthocyanin biosynthesis, and sugars enhance anthocyanin accumulation and F3H expression in some other plants. However, information about the relationship between sugars, anthocyanin accumulation and F3H expression in grape berries has been little reported. Present experiment was done with sliced grape berry system. The optimum fruit developmental stage, sugar concentration, and incubation time in sugar induction anthocyanin accumulation and F3H expression were determined. Mannose and 2-deoxyglucose, glucose analogs known to be phosphorylated by hexokinase but are poorly metabolized, obviously induced the anthocyanin accumulation and F3H expression, whereas 3-O-methylglucose and 6-deoxyglucose, glucose analogs transported inside the cell but not substrates for hexokinase, did not induce them. Glucosamine and mannoheptulose, the specific inhibitors of hexokinase, blocked the activation induced by sugar on both anthocyanin accumulation and F3H expression.

Keywords

Anthocyanin Flavanone 3-hydroxylase Sliced grape berry system Hexokinase Sugar signaling 

Abbreviations

CHS

Chalcone synthase

DAF

Day after full bloom

F3H

Flavanone 3-hydroxylase

PAL

Phenylalanine ammonia lyase

RT-PCR

Reverse transcription-polymerase chain reaction

Notes

Acknowledgments

This research was supported by major program of Beijing Municipal Science & Technology Commission (No. D07060500160701).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Yanjun Zheng
    • 1
  • Li Tian
    • 1
  • Hongtao Liu
    • 1
    • 2
  • Qiuhong Pan
    • 1
  • Jicheng Zhan
    • 1
  • Weidong Huang
    • 1
  1. 1.College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingChina
  2. 2.Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijingChina

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