Plant Growth Regulation

, Volume 58, Issue 2, pp 153–162 | Cite as

Short-term effects of experimental warming and enhanced ultraviolet-B radiation on photosynthesis and antioxidant defense of Picea asperata seedlings

  • Chao Han
  • Qing LiuEmail author
  • Yan Yang
Original Paper


Picea asperata is a dominant tree species in the southeast of the Tibetan Plateau of China. This paper studies the short-term effects of warming, enhanced UV-B (290–315 nm) and their combination on growth, photosynthesis and antioxidant defense system (AOS) of P. asperata seedlings. The experimental design included two levels of UV-B (ambient UV-B and enhanced by 30% UV-B) and two levels of temperature (ambient temperature and warmed temperature by 1.74°C). Although enhanced UV-B increased the efficiency of antioxidant defense system (AOS) including UV-B absorbing compounds, carotenoids, and antioxidant enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD), it induced over production of reactive oxygen species (ROS) and oxidative stress eventually. Moreover, enhanced UV-B reduced growth, chlorophyll content and net photosynthetic rate (A). Warming did not significantly affect dry mass accumulation of P. asperata seedlings while it accelerated stem elongation and stimulated A. Furthermore, warming alleviated the harmful effects of enhanced UV-B on the growth and photosynthesis. It also increased the antioxidant capacities of seedlings exposed to enhanced UV-B. Our results showed that the growth of P. asperata seedlings was inhibited by a combination of enhanced UV-B and warming, however, to some extent warming alleviated UV-B effects.


Picea asperata Photosynthesis Antioxidant defense Warming UV-B 



Net photosynthetic rate


Antioxidant defense system


Ascorbate peroxidase




Intercellular CO2 concentration


Stomatal conductance to vapour


Hydrogen peroxide




Nitroblue tetrazolium


Superoxide anion radicals




Reactive oxygen species


Superoxide dismutase





During this work, the senior author was supported by the National Natural Science Foundation of China (No.30530630), the Talent Plan of the Chinese Academy of Sciences and “Knowledge Innovation Engineering” of the Chinese Academy of Sciences.

Supplementary material

10725_2009_9363_MOESM1_ESM.doc (2.2 mb)
(DOC 2239 kb)


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Chengdu Institute of BiologyChinese Academy of SciencesChengduPeople’s Republic of China
  2. 2.Graduate School of the Chinese Academy of ScienceBeijingPeople’s Republic of China

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