Plant Growth Regulation

, 57:293 | Cite as

Simultaneous UPLC MS/MS analysis of endogenous jasmonic acid, salicylic acid, and their related compounds

  • Hideyuki Matsuura
  • Arata Aoi
  • Chizuru Satou
  • Mino Nakaya
  • Chikara Masuta
  • Kensuke Nabeta
Original Paper


Jasmonic acid (JA) and salicylic acid (SA) are plant hormones involved in plant growth and development. Recent studies demonstrated that presence of a complex interplay between JA and SA signaling pathways to response to pathogenesis attack and biotic stresses. To our best knowledge, no method has existed for simultaneous analyses of JA, SA, and their related compounds. Especially, the glucosides are thought to be the storages or the inactivated compounds, but their contribution should be considered for elucidating the amount of the aglycons. It is also valuable for measuring the endogenous amount of phenylalanine, cinnamic acid, and benzoic acid that are the biosynthetic intermediates of SA due to the existence of isochorismate pathway to synthesize SA. We established this method using deuterium labeled compounds as internal standards. This is the first report of simultaneous analysis of endogenous JA, SA, and their related compounds. Measuring the endogenous JA, SA, and their related compounds that had been accumulated in tobacco plants proved the practicality of the newly developed method. It was demonstrated that accumulation of JA, SA and their related compounds were induced in both case of TMV infection and abiotic stresses.


Jasmonic acid Salicylic acid Pathogenesis attack 



Jasmonic acid


Multiple reaction monitoring


Mass spectrometry


Tandem mass spectrometry


Salicylic acid


Salicylic acid glucoside


Tuberonic acid


Tuberonic acid glucoside


Tobacco mosaic virus


Ultra performance liquid chromatography



The authors are grateful to Mr. Kenji Watanabe and Dr. Eri Fukushi for measuring the NMR and mass spectra.


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Hideyuki Matsuura
    • 1
  • Arata Aoi
    • 1
  • Chizuru Satou
    • 1
  • Mino Nakaya
    • 1
  • Chikara Masuta
    • 2
  • Kensuke Nabeta
    • 1
  1. 1.Laboratory of Bioorganic Chemistry, Division of Applied Bioscience, Research Faculty of AgricultureHokkaido UniversitySapporoJapan
  2. 2.Laboratory of Cell Biology and Manipulation, Division of Applied Bioscience, Research Faculty of AgricultureHokkaido UniversitySapporoJapan

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