Plant Growth Regulation

, 57:223 | Cite as

Effect of 1-methylcyclopropene on expression of genes for ethylene biosynthesis enzymes and ethylene receptors in post-harvest broccoli

  • Gang Ma
  • Ran Wang
  • Cheng-Rong Wang
  • Masaya Kato
  • Kazuki Yamawaki
  • Fei-fei Qin
  • Hui-Lian Xu
Original Paper

Abstract

The effects of 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, on the senescence of broccoli (Brassica oleracea, L. var. italica) after harvest were studied, and its possible molecular mechanism was discussed. The results showed that 1-MCP treatment delayed the yellowing of broccoli florets, inhibited the activities of 1-aminocyclopropane-1-carboxylate acid (ACC) oxidase (ACO), and delayed the peaks in the ACC synthase (ACS) activity and ACC concentration. In addition, exogenous ethylene treatment did not accelerate yellowing in the florets pretreated with 1-MCP. The gene expression pattern of enzymes involved in ethylene biosynthesis and ethylene receptors in broccoli florets after harvest was investigated. 1-MCP treatment significantly decreased the expression of BO-ACS1, BO-ACS2, BO-ACO1, BO-ERS, BO-ETR1 and BO-ETR2. 1-MCP delayed the senescing process of broccoli by inhibiting the activities of enzymes involved in ethylene biosynthesis and gene expression of these enzymes and of ethylene receptors at the transcript level.

Keywords

Broccoli 1-Methylcyclopropene (1-MCP) Ethylene Yellowing Real-time PCR 

Abbreviations

1-MCP

1-Methylcyclopropene

ACC

1-Aminocyclopropane-1-carboxylate acid

ACO

ACC oxidase

ACS

ACC synthase

CA

Controlled atmosphere

MA

Modified atmosphere

SAM

S-adenosyl-l-methionine

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30370976), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, the Natural Science Foundation of Shandong Province, People’s Republic of China (No. Y2002D04), and the Natural Science Foundation of Qingdao, People’s Republic of China (No. 06-2-2-15-jch).

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Gang Ma
    • 1
    • 2
  • Ran Wang
    • 1
  • Cheng-Rong Wang
    • 1
  • Masaya Kato
    • 3
  • Kazuki Yamawaki
    • 3
  • Fei-fei Qin
    • 1
  • Hui-Lian Xu
    • 4
  1. 1.College of HorticultureQingdao Agricultural UniversityQingdaoPeople’s Republic of China
  2. 2.The United Graduate School of Agricultural ScienceGifu University (Shizuoka University)GifuJapan
  3. 3.Department of Biological and Environmental Sciences, Faculty of AgricultureShizuoka UniversityShizuokaJapan
  4. 4.International Nature Farming Research CenterNaganoJapan

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