Plant Growth Regulation

, 57:21 | Cite as

Caffeic acid inhibits in vitro rooting in mung bean [Vigna radiata (L.) Wilczek] hypocotyls by inducing oxidative stress

  • Harminder Pal Singh
  • Shalinder Kaur
  • Daizy R. Batish
  • Ravinder Kumar Kohli
Original Paper


Caffeic acid (CA), which is ubiquitously present in plants, is a potent phytotoxin affecting plant growth and physiology. The aim of our study was to investigate whether CA-induced inhibition of adventitious root formation (ARF) in mung bean {Vigna radiata (L.) Wilczek [Phaseolus aureus Roxb.]} involves the induction of conventional stress responses. The effect of CA (0–1000 μM) on ARF in mung bean was determined by measuring the generation of reactive oxygen species (ROS) in terms of malondialdehyde and hydrogen peroxide (H2O2) content, root oxidizability and changes in levels of antioxidant enzymes. Our results show that CA significantly enhanced MDA content, indicating severe lipid peroxidation, and increased H2O2 accumulation and root oxidizability in the lower rooted hypocotylar region (LRHR) of mung bean, thereby inducing oxidative stress and cellular damage. In response to CA, there was a significant upregulation in the activities of scavenging enzymes, such as superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, catalase and glutathione reductase, in LRHRs of mung bean. Based on these results, we conclude that CA inhibits ARF in mung bean hypocotyls by inducing ROS-generated oxidative stress and upregulating the activities of antioxidant enzymes.


Adventitious root formation Antioxidant enzymes Mung bean Oxidative damage Oxidative stress markers Reactive oxygen species Rooting mechanism 



Ascorbate peroxidase


Adventitious root formation


Caffeic acid




Ethylenediaminetetraacetic acid


Enzyme unit


Fresh weight


Guaiacol peroxidase


Glutathione reductase


Glutathione reduced


Glutathione oxidized


Hydrogen peroxide


Lipid peroxidation


Lower rooted hypocotylar region




Nicotinamide adenine dinucleotide phosphate reduced


Nitroblue tetrazolium


Post-expression stage




Root expression stage


Root initiation stage


Root oxidizability


Reactive oxygen species


Superoxide dismutase


Thiobarbituric acid reactive substance


2,3,5-Triphenyl tetrazolium chloride salt


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Centre for Environment and Vocational StudiesPanjab UniversityChandigarhIndia
  2. 2.Department of BotanyPanjab UniversityChandigarhIndia

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