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Plant Growth Regulation

, 57:21 | Cite as

Caffeic acid inhibits in vitro rooting in mung bean [Vigna radiata (L.) Wilczek] hypocotyls by inducing oxidative stress

  • Harminder Pal Singh
  • Shalinder Kaur
  • Daizy R. Batish
  • Ravinder Kumar Kohli
Original Paper

Abstract

Caffeic acid (CA), which is ubiquitously present in plants, is a potent phytotoxin affecting plant growth and physiology. The aim of our study was to investigate whether CA-induced inhibition of adventitious root formation (ARF) in mung bean {Vigna radiata (L.) Wilczek [Phaseolus aureus Roxb.]} involves the induction of conventional stress responses. The effect of CA (0–1000 μM) on ARF in mung bean was determined by measuring the generation of reactive oxygen species (ROS) in terms of malondialdehyde and hydrogen peroxide (H2O2) content, root oxidizability and changes in levels of antioxidant enzymes. Our results show that CA significantly enhanced MDA content, indicating severe lipid peroxidation, and increased H2O2 accumulation and root oxidizability in the lower rooted hypocotylar region (LRHR) of mung bean, thereby inducing oxidative stress and cellular damage. In response to CA, there was a significant upregulation in the activities of scavenging enzymes, such as superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, catalase and glutathione reductase, in LRHRs of mung bean. Based on these results, we conclude that CA inhibits ARF in mung bean hypocotyls by inducing ROS-generated oxidative stress and upregulating the activities of antioxidant enzymes.

Keywords

Adventitious root formation Antioxidant enzymes Mung bean Oxidative damage Oxidative stress markers Reactive oxygen species Rooting mechanism 

Abbreviations

APX

Ascorbate peroxidase

ARF

Adventitious root formation

CA

Caffeic acid

CAT

Catalase

EDTA

Ethylenediaminetetraacetic acid

EU

Enzyme unit

FW

Fresh weight

GPx

Guaiacol peroxidase

GR

Glutathione reductase

GSH

Glutathione reduced

GSSG

Glutathione oxidized

H2O2

Hydrogen peroxide

LP

Lipid peroxidation

LRHR

Lower rooted hypocotylar region

MDA

Malondialdehyde

NADPH

Nicotinamide adenine dinucleotide phosphate reduced

NBT

Nitroblue tetrazolium

PE

Post-expression stage

PO43−

Phosphate

RE

Root expression stage

RI

Root initiation stage

RO

Root oxidizability

ROS

Reactive oxygen species

SOD

Superoxide dismutase

TBARS

Thiobarbituric acid reactive substance

TTC

2,3,5-Triphenyl tetrazolium chloride salt

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Centre for Environment and Vocational StudiesPanjab UniversityChandigarhIndia
  2. 2.Department of BotanyPanjab UniversityChandigarhIndia

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