Plant Growth Regulation

, 55:221 | Cite as

Effects of plant growth regulators and sucrose on post harvest physiology, membrane stability and vase life of cut spikes of gladiolus

  • Alka Singh
  • Jitendra Kumar
  • Pushpendra Kumar
Original Paper


Effects of post-harvest application of two plant growth regulators viz., gibberellic acid (GA3) and benzyl adenine (BA) with sucrose in the vase solution on cell membrane stability and vase life of gladiolus were investigated. The vase solution treatment combinations of GA3 and BA with sucrose significantly increased the membrane stability index and enhanced the vase life as compared to the sucrose alone treatments or the controls. Vase solution treatment of GA3 (50 mg l−1), followed by BA (50 mg l−1) with sucrose (50 g l−1) significantly increased solution uptake, fresh weight and dry weight of cut spikes. The same treatments also enhanced the concentration of reducing and non-reducing sugars in gladioli petals 4 days after treatment (DAT). Cut spikes in vase solution enriched with 50 mg l−1 GA3 + 50 g l−1 sucrose showed higher antioxidative enzyme activities of superoxide dismutase (SOD) and glutathione reductase (GR), lower lipoxygenase (LOX) activity and lipid peroxidation (measured as TBARS). Petal membrane stability index was also highest in cut spikes 6 DAT with 50 mg l−1 GA3 + 50 g l−1 sucrose vase solution. Treatment of gladiolus cut spikes with 50 mg l−1 GA3 + 50 g l−1 sucrose vase solution showed two fold increase in vase life and improved flower quality with a higher number of open flower per spike at any one time. These results suggest that post-harvest application of GA3 (50 mg l−1) with sucrose (50 g l−1) maintains higher spike fresh and dry weight, improves anti-oxidative defence, stabilizes membrane integrity leading to a delay in petal cell death.


Benzyladenine Gibberellic acid Glutathione reductase Superoxide dismutase Lipoxygenase TBARS Membrane stability index Post-harvest Vase life 



The authors are thankful to Dr. M. U. Charaya, Reader, CCS University, Meerut, UP, India for critically going through the manuscript and giving suggestions.


  1. Albert DI, Kokkenlink S, Vander Veen BE, Valk AW, Schram AC, Douma AC (1992) Purification and characterization of two lipoxygeanse isoenzymes from germinating barley. Biochimica et Biophysica Acta 1120:97–104Google Scholar
  2. Balibrea Lara ME, Gonzalez Garcia MC, Fatima T, Ehness R, Lee TK, Proels R, Roitsch T (2004) Extracellular invertase is an essential component of cytokyanin mediated delay of senescence. Plant Cell 16:1276–1287PubMedCrossRefGoogle Scholar
  3. Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101CrossRefGoogle Scholar
  4. Eason JR (2002) Sandersonia aurantiaca: an evaluation of post harvest pulsing solutions to maximize cut flower quality. NZ J Crop Hortic Sci 30:273–279Google Scholar
  5. Elanchezhian R, Srivastava GC (2000) Effect of growth regulators on senescence of chrysanthemum flowers. Indian J Plant Physiol 6:233–243Google Scholar
  6. Emongor VE (2004) Effects of gibberellic acid on post-harvest quality and vaselife of gerbera cut flowers (Gerbera jamesonii). J Agron 3(3):191–195CrossRefGoogle Scholar
  7. Gomez JM, Hernandez JA, Jimenez A, del Rio LA, Sevilla F (1999) Differential response of antioxidative enzymes of chloroplasts and mitochondria to long-term NaCl stress of pea plants. Free Radic Res Suppl 31:11–18CrossRefGoogle Scholar
  8. Halevy AH, Mayak S (1981) Senescence and post harvest physiology of cut flowers. Part II. Hortic Rev 3:59–143Google Scholar
  9. Han SS (2001) Benzyladenine and gibberellins improve post harvest quality of cut Asiatic and Oriental lilies. HortScience 36(4):741–745Google Scholar
  10. Heath RL, Parker L (1968) Photoperoxidation in isolated chloroplast I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198PubMedCrossRefGoogle Scholar
  11. Hernandez JA, del Rio LA, Sevilla F (1994) Salt stress induced changes in superoxide dismutase isozymes in leaves and mesophyll protoplasts from Vigna radiata (L.). New Phytol 126:37–44CrossRefGoogle Scholar
  12. Hossain Z, Mandal AKA, Datta SK, Biswas AK (2006) Decline in ascorbate peroxidase activity—a prerequisite factor for tepal senescence in gladiolus. J Plant Physiol 163(2):186–194PubMedCrossRefGoogle Scholar
  13. Klapheck S (1988) Homoglutathione: isolation, quantification and occurrence in legumes. Plant Physiol 74:727–732CrossRefGoogle Scholar
  14. Koch KE (1996) Carbohydrate modulated gene expression in plants. Ann Rev Plant Physiol Plant Mol Biol 47:509–540CrossRefGoogle Scholar
  15. Kumar N, Srivastava GC, Dixit K (2007) Role of superoxide dismutases during petal senescence in rose (Rosa hybrida L.). J Hortic Sci Biotechnol 82(5):673–678Google Scholar
  16. Kwon H, Kim K (2000) Inhibition of lipooxygenase activity and microorganism growth in cut Freesia by pulsing treatment. J Korean Soc Hortic Sci 41(2):135–138Google Scholar
  17. Mayak S, Halevy AH, Kats M (1972) Correlative changes in phytohormones in relation to senescence process in rose petals. Physiol Plant 27:1–4Google Scholar
  18. Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153:375–380Google Scholar
  19. Ohlsson AB, Berglund T (1998) Antioxidative effects of gibberellic acid. In: Proceeding of the 11th congress of the federation of European societies of plant physiology, held at Varna, Bulgaria, 7–11 Sept 1998Google Scholar
  20. Peary JS, Prince TA (1990) Floral lipoxygenase: activity during senescence and inhibition by phenidone. J Am Soc Hortic Sci 115(3):455–457Google Scholar
  21. Sabehat A, Zeislin N (1994) GA3 effects on post harvest alterations in cell membranes of rose (Rosa × hybrida) petals. J Plant Physiol 144:513–517Google Scholar
  22. Scandalios JG (1993) Oxygen stress and superoxide dismutases. Plant Physiol 101:7–12PubMedGoogle Scholar
  23. Serek M, Reid MS (1997) Use of growth regulators for improving the post harvest quality of ornamentals. Perishables Handling 92:7–8Google Scholar
  24. Smith IK, Vierheller TL, Thurne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis(2-nitrobenzoic acid). Anal Biochem 175:408–413PubMedCrossRefGoogle Scholar
  25. van der Meuler-Muisers JJM, van Overen JC, van der Plas LHW, Van Tuyl JM (2001) Post harvest flower development in asiatic hybrid lilies as related to petal carbohydrate status. Post Harvest Biol Technol 21:201–211CrossRefGoogle Scholar
  26. van Doorn WG (2004) Is petal senescence due to sugar starvation? Plant Physiol 134:35–42PubMedCrossRefGoogle Scholar
  27. Waithaka K, Dodge LL, Reid SS (2001) Carbohydrate traffic during opening of gladiolus florets. J Hortic Sci Biotechnol 76:120–125Google Scholar
  28. Whitehead CH (1994) Ethylene sensitivity and flower senescence. In: Scott RJ, Stead AD (eds), Molecular and cellular aspects of plant reproduction. Cambridge University Press, Cambridge, pp 269–285Google Scholar
  29. Wood A, Pleg LG (1974) Alteration of liposomal membrane fluidity by gibberellic acid. Aust J Plant Physiol 1(1):31–40CrossRefGoogle Scholar
  30. Yu SM (1999) Cellular and genetic responses of plants to sugar starvation. Plant Physiol 121:687–693PubMedCrossRefGoogle Scholar
  31. Zhang ZH, Guo WM (1998) Regulation of 6-BA on membrane permeability of cut chrysanthemum in vase periods. Adv Hortic 2:697–701Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Floriculture & Landscaping, ACHFN.A.U.NavsariIndia
  2. 2.Department of HorticultureC.C.S. UniversityMeerutIndia
  3. 3.Department of BiotechnologyS.V.B.P.U.A & TMeerutIndia

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