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Plant Growth Regulation

, Volume 55, Issue 3, pp 165–167 | Cite as

A rapid effect of applied brassinolide on abscisic acid concentrations in Brassica napus leaf tissue subjected to short-term heat stress

  • Leonid V. KurepinEmail author
  • Mirwais M. Qaderi
  • Thomas G. Back
  • David M. Reid
  • Richard P. Pharis
Brief Communication

Abstract

Three-week old canola (Brassica napus L.) seedlings grown at 20/16°C (day/night) were subjected to short-term (4 and 8 h) heat stress (45°C) or maintained at a normal temperature of 20°C. Half of the plants under each treatment received a 10−6 M solution of brassinolide (BL) 1 h prior to beginning the temperature treatments. The concentration (ng/g dry weight) of endogenous abscisic acid (ABA) was subsequently determined in young leaves via the stable isotope dilution method. Applied BL had no effect on endogenous ABA for plants maintained at normal temperatures. However, ABA concentration was significantly elevated by heat stress alone and doubled by heat stress + BL. These results suggest that the well-known enhancement of tolerance to high temperature stress that can be obtained by BL or 24-epi-BL applications may be caused by a brassinosteroid-induced elevation in endogenous ABA concentration.

Keywords

Brassinolide Brassinosteroids Abscisic acid Heat stress 

Notes

Acknowledgements

This work was funded by NSERC (Canada) grants to RPP, DMR and TGB.

References

  1. Abass M, Rajashekar CB (1993) Abscisic acid accumulation in leaves and cultured cells during heat acclimation in grapes. HortScience 28:50–52Google Scholar
  2. Chandler PM, Robertson M (1994) Gene expression regulated by abscisic acid and its relation to stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 45:113–141CrossRefGoogle Scholar
  3. Crafts-Brandner SJ, Salvucci ME (2000) Rubisco activase constrains the photosynthetic potential of leaves at high temperature and CO2. Proc Natl Acad Sci USA 97:13430–13435PubMedCrossRefGoogle Scholar
  4. Dhaubhadel S, Chaudhary S, Dobinson KF, Krishna P (1999) Treatment with 24-epibrassinolide, a brassinosteroid, increases the basic thermotolerance of Brassica napus and tomato seedlings. Plant Mol Biol 40:333–342PubMedCrossRefGoogle Scholar
  5. Dhaubhadel S, Browning KS, Gallie DR, Krishna P (2002) Brassinosteroid functions to protect the translational machinery and heat shock protein synthesis following thermal stress. Plant J 29:681–691PubMedCrossRefGoogle Scholar
  6. Eun J-S, Kuraishi S, Sakurai N (1989) Changes in levels of auxin and abscisic acid and the evolution of ethylene in squash hypocotyls after treatment with brassinolide. Plant Cell Physiol 30:807–810Google Scholar
  7. Gaskin P, MacMillian J (1991) GC-MS of the gibberellins and related compounds. Methodology and a library of spectra. University of Bristol (Cantock’s Enterprises), Bristol, UKGoogle Scholar
  8. Katsumi M (1991) Physiological modes of brassinolide action in cucumber hypocotyl growth. In: Cutler HG, Yokota T, Adam G (eds) Brassinosteroids: chemistry, bioactivity and applications. ACS Symp Ser 474. American Chemical Society, Washington, DC, pp 246–254Google Scholar
  9. Khripach VA, Zhabinskii VN, de Groot AE (1999) Brassinosteroids: a new class of plant hormones. Academic Press, San Diego, pp 263–277Google Scholar
  10. Krishna P (2003) Brassinosteroid-mediated stress responses. J Plant Growth Regul 22:289–297PubMedCrossRefGoogle Scholar
  11. Kurepin LV, Pharis RP, Reid DM, Chinnappa CC (2006) Involvement of gibberellins in the stem elongation of sun and shade ecotypes of Stellaria longipes that is induced by low light irradiance. Plant Cell Environ 29:1319–1328PubMedCrossRefGoogle Scholar
  12. Nilsen ET, Orcutt DM (1996) The physiology of plants under stress: abiotic factors. John Wiley & Sons, Inc, New YorkGoogle Scholar
  13. Qaderi MM, Kurepin LV, Reid DM (2006) Growth and physiological responses of canola (Brassica napus) to three components of global climate change: temperature, carbon dioxide and drought. Physiol Plant 128:710–721CrossRefGoogle Scholar
  14. Sakurai A, Yokota T, Clouse SD (1999) Brassinosteroids: steroidal plant hormones. Springer-Verlag, Tokyo, pp 151–153Google Scholar
  15. Wilen RW, Sacco M, Gusta LV, Krishna P (1995) Effects of 24-epibrassinolide on freezing and thermotolerance of bromegrass (Bromus inermis) cell cultures. Physiol Plant 95:195–202CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Leonid V. Kurepin
    • 1
    Email author
  • Mirwais M. Qaderi
    • 1
  • Thomas G. Back
    • 2
  • David M. Reid
    • 1
  • Richard P. Pharis
    • 1
  1. 1.Department of Biological SciencesUniversity of CalgaryCalgaryCanada
  2. 2.Department of ChemistryUniversity of CalgaryCalgaryCanada

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