Advertisement

Plant Growth Regulation

, Volume 55, Issue 1, pp 29–34 | Cite as

Melatonin stimulates the expansion of etiolated lupin cotyledons

  • Josefa Hernández-Ruiz
  • Marino B. ArnaoEmail author
Original Paper

Abstract

Melatonin (N-acetyl-5-methoxytryptamine) is an indoleamine which is structurally related to tryptophan, serotonin and indole-3-acetic acid (IAA), among other important substances. Many studies have clearly demonstrated its presence in different plant organs, including roots, stems, leaves, flowers, fruits and seeds. Since it discovery in plants in 1995, authors have postulated many physiological roles for melatonin, although research into this molecule in plants is still in its infancy. The data presented in this study demonstrate that melatonin stimulates the expansion of etiolated cotyledons of lupin (Lupinus albus L.) to a similar extent to that observed for IAA but less than in the case of kinetin. Endogenous melatonin in imbibed cotyledons has been quantified using a liquid chromatography method with fluorescence detection and capacity of cotyledons to absorb melatonin has been determined. The observed effect of melatonin on lupin cotyledon expansion can be added to the other effects demonstrated by our group such as its role as growth promoter and rooting promotor in adventitious and lateral roots.

Keywords

Auxin Cotyledon IAA Melatonin Growth Lupinus 

Notes

Acknowledgments

This work was supported by project MCyT-BFU2006–00671 (co-financed by FEDER) and by project 502/PI/04 from Fundación SENECA (C.A.R.Murcia). J.H.R. has a contract with the University of Murcia.

References

  1. Arnao MB, Hernández-Ruiz J (2006) The physiological function of melatonin in plants. Plant Signal Behav 1:89–95PubMedGoogle Scholar
  2. Arnao MB, Hernández-Ruiz J (2007a) Melatonin promotes adventitious and lateral root regeneration in etiolated hypocotyls of Lupinus albus L. J Pineal Res 42:147–152PubMedCrossRefGoogle Scholar
  3. Arnao MB, Hernández-Ruiz J (2007b) Inhibition of ACC oxidase activity by melatonin and IAA in etiolated lupin hypocotyls. In: Ramina A, Chang C, Giovannoni J, Klee H, Perata P, Woltering E (eds) Advances in plant ethylene research, Dordrecht, pp 101–103Google Scholar
  4. Arnao MB, Hernández-Ruiz J (2007c) Melatonin in plants: more studies are necessary. Plant Signal Behav 2:381–382PubMedGoogle Scholar
  5. Dubbels R, Reiter RJ, Klenke E, Goebel A, Schnakenberg E, Ehlers C, Schiwara HW, Schloot W (1995) Melatonin in edible plants identified by radioimmunoassay and high performance liquid chromatography-mass spectrometry. J Pineal Res 18:28–31PubMedCrossRefGoogle Scholar
  6. Foulkes NS, Borjigin J, Snyder SH, Sassone-Corsi P (1997) Rhythmic transcription: the molecular basis of circadian melatonin synthesis. Trends Neurosci 20:487–492PubMedCrossRefGoogle Scholar
  7. Hardeland R, Fuhrberg B (1996) Ubiquitous melatonin: presence and effects in unicells, plants and animals. Trends Comp Biochem Physiol 2:25–45Google Scholar
  8. Hardeland R, Poeggeler B (2003) Non-vertebrate melatonin. J Pineal Res 34:233–241PubMedCrossRefGoogle Scholar
  9. Hardeland R, Pandi-Perumal SR, Poeggeler B (2007) Melatonin in plants. Focus on a vertebrate night hormone with cytoprotective properties. Funct Plant Sci Biotechnol 1:32–45Google Scholar
  10. Hernández-Ruiz J, Cano A, Arnao MB (2004) Melatonin: a growth-stimulating compound present in lupin tissues. Planta 220:140–144PubMedCrossRefGoogle Scholar
  11. Hernández-Ruiz J, Cano A, Arnao MB (2005) Melatonin acts as a growth-stimulating compound in some monocot species. J Pineal Res 39:137–142PubMedCrossRefGoogle Scholar
  12. Kolar J, Machackova I (2005) Melatonin in higher plants. Occurrence and possible functions. J Pineal Res 39:333–341PubMedCrossRefGoogle Scholar
  13. Kolar J, Machackova I, Illnerova H, Prinsen E, van Dongen W, van Onckelen HA (1995) Melatonin in higher plant determined by radioimmunoassay and high performance liquid chromatography-mass spectrometry-mass spectrometry. Biol Rhythm Res 26:406–409Google Scholar
  14. Kolar J, Machackova I, Eder J, Prinsen E, van Dongen W, van Onckelen H, Illnerova H (1997) Melatonin: occurrence and daily rhythm in Chenopodium rubrum. Phytochemistry 44:1407–1413CrossRefGoogle Scholar
  15. Kolar J, Johnson CH, Machackova I (2003) Exogenously applied melatonin affects flowering of the short-day plant Chenopodium rubrum. Physiol Plant 118:605–612CrossRefGoogle Scholar
  16. Letham DS (1971) Regulators of cell division in plant tissues. A cytokinin bioassay using excised radish cotyledons. Physiol Plant 25:391–396CrossRefGoogle Scholar
  17. Manchester LC, Tan DX, Reiter RJ, Park W, Monis K, Qi W (2000) High levels of melatonin in the seeds of edible plants. Possible function in germ tissue protection. Life Sci 67:3023–3029PubMedCrossRefGoogle Scholar
  18. Murch S, Saxena PK (2002) Melatonin: a potential regulator of plant growth and development? In vitro Cell Dev Biol Plant 38:531–536CrossRefGoogle Scholar
  19. Murch S, KrishnaRaj S, Saxena PK (2000) Tryptophan is a precursor for melatonin and serotonin biosynthesis in in vitro regenerated St. John’s wort (Hypericum perforatum L. cv. Anthos) plants. Plant Cell Rep 19:698–704CrossRefGoogle Scholar
  20. Reiter RJ (1993) The melatonin rhythm: both a clock and a calendar. Experientia 49:654–664PubMedCrossRefGoogle Scholar
  21. Reiter RJ, Tan DX, Manchester LC, Qi W (2001) Biochemical reactivity of melatonin with reactive oxygen and nitrogen species. Cell Biochem Biophys 34:237–256PubMedCrossRefGoogle Scholar
  22. Thomas JC, Katterman F (1992) 5-Bromodeoxyuridine inhibition of cytokinin induced radish cotyledon expansion. Plant Sci 83:143–148CrossRefGoogle Scholar
  23. Van Tassel DL, Roberts NJ, Lewy A, O’Neill SD (2001) Melatonin in plant organs. J Pineal Res 31:8–15PubMedCrossRefGoogle Scholar
  24. Wolf K, Kolar J, van Dongen W, van Onckelen H, Machackova I (2001) Daily profile of melatonin levels in Chenopodium rubrum depends on photoperiod. J Plant Physiol 158:1491–1493CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Plant Physiology, Faculty of BiologyUniversity of MurciaMurciaSpain

Personalised recommendations