Plant Growth Regulation

, 54:217 | Cite as

Isolation of 24-epibrassinolide from leaves of Aegle marmelos and evaluation of its antigenotoxicity employing Allium cepa chromosomal aberration assay

  • Nishi Sondhi
  • Renu Bhardwaj
  • Satwinderjeet Kaur
  • Neeraj Kumar
  • Bikram Singh
Original Paper


Brassinosteroids are of universal occurrence in plants. They have been reported to affect plant growth and development through a spectrum of physiological responses. Recently they are reported to confer resistance in plants against a number of biotic and abiotic stresses. In the present study, a brassinosteroid was isolated from Aegle marmelos Correa. (Rutaceae) which was characterized to be 24-epibrassinolide (EBL) using various spectroscopic techniques (TLC and ESI-MS analysis). It was evaluated for the antigenotoxicity against maleic hydrazide (MH) induced genotoxicity in Allium cepa chromosomal aberration assay. It was shown that the percentage of chromosomal aberrations induced by maleic hydrazide (0.01%) declined significantly with 24-epibrassinolide treatment. EBL (10−7 M) proved to be the most effective concentration with 91.8% inhibition. This is the first report on the isolation of 24-epibrassinolide from Aegle marmelos and its antigenotoxic effects against MH employing Allium cepa chromosomal aberration assay.


Antigenotoxicity Brassinosteroid 24-Epibrassinolide Maleic hydrazide Aegle marmelos 



This work is supported by grants from University Grants Commission, Government of India. Dr. P. S. Ahuja, Director, Institute of Himalayan Bioresource Technology (IHBT) Palampur (HP) is duly acknowledged for providing necessary lab facilities.


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Nishi Sondhi
    • 1
  • Renu Bhardwaj
    • 1
  • Satwinderjeet Kaur
    • 1
  • Neeraj Kumar
    • 2
  • Bikram Singh
    • 2
  1. 1.Department of Botanical and Environmental SciencesGuru Nanak Dev UniversityAmritsarIndia
  2. 2.Division of Natural Plant ProductsInstitute of Himalayan Bioresource TechnologyPalampurIndia

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